Isolation and clinical sample typing of human leptospirosis cases in Argentina
- Autores
- Chiani, Yosena; Jacob, Paulina; Varni, Vanina; Landolt, Noelia; Schmeling, María Fernanda; Pujato, Nazarena; Caimi, Karina; Vanasco, Bibiana N.
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients. - Fuente
- Infection, Genetics and Evolution 2016;37:245-251
- Materia
-
ARN Ribosómico 16S
Tipificación de Secuencias Multilocus
Serotipificación
Leptospira - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- none
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/1384
Ver los metadatos del registro completo
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Isolation and clinical sample typing of human leptospirosis cases in ArgentinaChiani, YosenaJacob, PaulinaVarni, VaninaLandolt, NoeliaSchmeling, María FernandaPujato, NazarenaCaimi, KarinaVanasco, Bibiana N.ARN Ribosómico 16STipificación de Secuencias MultilocusSerotipificaciónLeptospiraFil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients.2016-01info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1567-1348http://sgc.anlis.gob.ar/handle/123456789/138410.1016/j.meegid.2015.11.033Infection, Genetics and Evolution 2016;37:245-251reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseasesnoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:18Zoai:sgc.anlis.gob.ar:123456789/1384Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:18.877Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
title |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
spellingShingle |
Isolation and clinical sample typing of human leptospirosis cases in Argentina Chiani, Yosena ARN Ribosómico 16S Tipificación de Secuencias Multilocus Serotipificación Leptospira |
title_short |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
title_full |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
title_fullStr |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
title_full_unstemmed |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
title_sort |
Isolation and clinical sample typing of human leptospirosis cases in Argentina |
dc.creator.none.fl_str_mv |
Chiani, Yosena Jacob, Paulina Varni, Vanina Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Caimi, Karina Vanasco, Bibiana N. |
author |
Chiani, Yosena |
author_facet |
Chiani, Yosena Jacob, Paulina Varni, Vanina Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Caimi, Karina Vanasco, Bibiana N. |
author_role |
author |
author2 |
Jacob, Paulina Varni, Vanina Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Caimi, Karina Vanasco, Bibiana N. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
ARN Ribosómico 16S Tipificación de Secuencias Multilocus Serotipificación Leptospira |
topic |
ARN Ribosómico 16S Tipificación de Secuencias Multilocus Serotipificación Leptospira |
dc.description.none.fl_txt_mv |
Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina. Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina. Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients. |
description |
Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1567-1348 http://sgc.anlis.gob.ar/handle/123456789/1384 10.1016/j.meegid.2015.11.033 |
identifier_str_mv |
1567-1348 10.1016/j.meegid.2015.11.033 |
url |
http://sgc.anlis.gob.ar/handle/123456789/1384 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases |
dc.rights.none.fl_str_mv |
none info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
none |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Infection, Genetics and Evolution 2016;37:245-251 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
reponame_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
collection |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
institution |
ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
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1844621855829262336 |
score |
12.559606 |