Isolation and clinical sample typing of human leptospirosis cases in Argentina

Autores
Chiani, Yosena; Jacob, Paulina; Varni, Vanina; Landolt, Noelia; Schmeling, María Fernanda; Pujato, Nazarena; Caimi, Karina; Vanasco, Bibiana N.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients.
Fuente
Infection, Genetics and Evolution 2016;37:245-251
Materia
ARN Ribosómico 16S
Tipificación de Secuencias Multilocus
Serotipificación
Leptospira
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1384

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oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/1384
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Isolation and clinical sample typing of human leptospirosis cases in ArgentinaChiani, YosenaJacob, PaulinaVarni, VaninaLandolt, NoeliaSchmeling, María FernandaPujato, NazarenaCaimi, KarinaVanasco, Bibiana N.ARN Ribosómico 16STipificación de Secuencias MultilocusSerotipificaciónLeptospiraFil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients.2016-01info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1567-1348http://sgc.anlis.gob.ar/handle/123456789/138410.1016/j.meegid.2015.11.033Infection, Genetics and Evolution 2016;37:245-251reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseasesnoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:18Zoai:sgc.anlis.gob.ar:Publications/123456789/1384Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:18.88Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Isolation and clinical sample typing of human leptospirosis cases in Argentina
title Isolation and clinical sample typing of human leptospirosis cases in Argentina
spellingShingle Isolation and clinical sample typing of human leptospirosis cases in Argentina
Chiani, Yosena
ARN Ribosómico 16S
Tipificación de Secuencias Multilocus
Serotipificación
Leptospira
title_short Isolation and clinical sample typing of human leptospirosis cases in Argentina
title_full Isolation and clinical sample typing of human leptospirosis cases in Argentina
title_fullStr Isolation and clinical sample typing of human leptospirosis cases in Argentina
title_full_unstemmed Isolation and clinical sample typing of human leptospirosis cases in Argentina
title_sort Isolation and clinical sample typing of human leptospirosis cases in Argentina
dc.creator.none.fl_str_mv Chiani, Yosena
Jacob, Paulina
Varni, Vanina
Landolt, Noelia
Schmeling, María Fernanda
Pujato, Nazarena
Caimi, Karina
Vanasco, Bibiana N.
author Chiani, Yosena
author_facet Chiani, Yosena
Jacob, Paulina
Varni, Vanina
Landolt, Noelia
Schmeling, María Fernanda
Pujato, Nazarena
Caimi, Karina
Vanasco, Bibiana N.
author_role author
author2 Jacob, Paulina
Varni, Vanina
Landolt, Noelia
Schmeling, María Fernanda
Pujato, Nazarena
Caimi, Karina
Vanasco, Bibiana N.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARN Ribosómico 16S
Tipificación de Secuencias Multilocus
Serotipificación
Leptospira
topic ARN Ribosómico 16S
Tipificación de Secuencias Multilocus
Serotipificación
Leptospira
dc.description.none.fl_txt_mv Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Jacob, Paulina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Varni, Vanina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Landolt, Noelia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Schmeling, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Pujato, Nazarena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Fil: Caimi, Karina. Instituto Nacional de Tecnología Agrícola. Instituto de Biotecnología, Castelar, Buenos Aires; Argentina.
Fil: Vanasco, Bibiana N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients.
description Fil: Chiani, Yosena. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1567-1348
http://sgc.anlis.gob.ar/handle/123456789/1384
10.1016/j.meegid.2015.11.033
identifier_str_mv 1567-1348
10.1016/j.meegid.2015.11.033
url http://sgc.anlis.gob.ar/handle/123456789/1384
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Infection, Genetics and Evolution 2016;37:245-251
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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