Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice

Autores
Fernandez-Brando, Romina J; Cabrera, Gabriel; Baschkier, Ariela; Mejías, M P; Panek, C A; Miliwebsky, Elizabeth; Abrey-Recalde, M J; Bentancor, L; Ramos, María Victoria; Rivas, Marta; Palermo, M.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Mejías, María Pilar. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Bentancor, Leticia Verónica. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, Marina Sandra. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Hemolytic uremic syndrome (HUS) is the major complication of gastrointestinal infections with enterohemorrhagic Escherichia coli (EHEC) and is mediated by the production of Shiga toxins (Stx). Although it has been previously reported that not only HUS patients but healthy children have anti-Stx antibodies, very little is known about how these infections impact on mucosal immune system to generate a specific immune response. This work aimed to evaluate the immune responses elicited after a single oral dose of EHEC in a mouse model of HUS at weaning. We found sequential activation of T and B lymphocytes together with an increased percentage of IgA-bearing B cells in Peyer's patches and mesenteric lymph nodes. We also found fecal anti-EHEC IgA and serum anti-Stx2 IgG in EHEC-inoculated mice. Besides, these mice were partially protected against an intravenous challenge with Stx2. These data demonstrate that one episode of EHEC infection is enough to induce activation in the gut-associated lymphoid tissue, especially the B cell compartment, and lead to the production of specific IgA in mucosal tissue and the generation of systemic protection against Stx2 in a percentage of intragastrically inoculated mice. These data also support the epidemiologic observation that a second episode of HUS is very rare.
Fuente
Medical Microbiology and Immunology 2014;203(3):145-154
pdf
Materia
Escherichia coli Enterohemorrágica
Inmunidad Mucosa
Escherichia coli Shiga-Toxigénica
Toxina Shiga
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/2247
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/2247
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in miceFernandez-Brando, Romina JCabrera, GabrielBaschkier, ArielaMejías, M PPanek, C AMiliwebsky, ElizabethAbrey-Recalde, M JBentancor, LRamos, María VictoriaRivas, MartaPalermo, M.Escherichia coli EnterohemorrágicaInmunidad MucosaEscherichia coli Shiga-ToxigénicaToxina ShigaFil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Mejías, María Pilar. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Bentancor, Leticia Verónica. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Palermo, Marina Sandra. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Hemolytic uremic syndrome (HUS) is the major complication of gastrointestinal infections with enterohemorrhagic Escherichia coli (EHEC) and is mediated by the production of Shiga toxins (Stx). Although it has been previously reported that not only HUS patients but healthy children have anti-Stx antibodies, very little is known about how these infections impact on mucosal immune system to generate a specific immune response. This work aimed to evaluate the immune responses elicited after a single oral dose of EHEC in a mouse model of HUS at weaning. We found sequential activation of T and B lymphocytes together with an increased percentage of IgA-bearing B cells in Peyer's patches and mesenteric lymph nodes. We also found fecal anti-EHEC IgA and serum anti-Stx2 IgG in EHEC-inoculated mice. Besides, these mice were partially protected against an intravenous challenge with Stx2. These data demonstrate that one episode of EHEC infection is enough to induce activation in the gut-associated lymphoid tissue, especially the B cell compartment, and lead to the production of specific IgA in mucosal tissue and the generation of systemic protection against Stx2 in a percentage of intragastrically inoculated mice. These data also support the epidemiologic observation that a second episode of HUS is very rare.2014-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1432-1831http://sgc.anlis.gob.ar/handle/123456789/224710.1007/s00430-013-0325-yMedical Microbiology and Immunology 2014;203(3):145-154pdfreponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISMedical microbiology and immunologynoneinfo:eu-repo/semantics/openAccesseng2025-09-04T11:18:22Zoai:sgc.anlis.gob.ar:123456789/2247Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:18:23.022Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
title Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
spellingShingle Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
Fernandez-Brando, Romina J
Escherichia coli Enterohemorrágica
Inmunidad Mucosa
Escherichia coli Shiga-Toxigénica
Toxina Shiga
title_short Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
title_full Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
title_fullStr Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
title_full_unstemmed Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
title_sort Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
dc.creator.none.fl_str_mv Fernandez-Brando, Romina J
Cabrera, Gabriel
Baschkier, Ariela
Mejías, M P
Panek, C A
Miliwebsky, Elizabeth
Abrey-Recalde, M J
Bentancor, L
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author Fernandez-Brando, Romina J
author_facet Fernandez-Brando, Romina J
Cabrera, Gabriel
Baschkier, Ariela
Mejías, M P
Panek, C A
Miliwebsky, Elizabeth
Abrey-Recalde, M J
Bentancor, L
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author_role author
author2 Cabrera, Gabriel
Baschkier, Ariela
Mejías, M P
Panek, C A
Miliwebsky, Elizabeth
Abrey-Recalde, M J
Bentancor, L
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli Enterohemorrágica
Inmunidad Mucosa
Escherichia coli Shiga-Toxigénica
Toxina Shiga
topic Escherichia coli Enterohemorrágica
Inmunidad Mucosa
Escherichia coli Shiga-Toxigénica
Toxina Shiga
dc.description.none.fl_txt_mv Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Mejías, María Pilar. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Bentancor, Leticia Verónica. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, Marina Sandra. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Hemolytic uremic syndrome (HUS) is the major complication of gastrointestinal infections with enterohemorrhagic Escherichia coli (EHEC) and is mediated by the production of Shiga toxins (Stx). Although it has been previously reported that not only HUS patients but healthy children have anti-Stx antibodies, very little is known about how these infections impact on mucosal immune system to generate a specific immune response. This work aimed to evaluate the immune responses elicited after a single oral dose of EHEC in a mouse model of HUS at weaning. We found sequential activation of T and B lymphocytes together with an increased percentage of IgA-bearing B cells in Peyer's patches and mesenteric lymph nodes. We also found fecal anti-EHEC IgA and serum anti-Stx2 IgG in EHEC-inoculated mice. Besides, these mice were partially protected against an intravenous challenge with Stx2. These data demonstrate that one episode of EHEC infection is enough to induce activation in the gut-associated lymphoid tissue, especially the B cell compartment, and lead to the production of specific IgA in mucosal tissue and the generation of systemic protection against Stx2 in a percentage of intragastrically inoculated mice. These data also support the epidemiologic observation that a second episode of HUS is very rare.
description Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1432-1831
http://sgc.anlis.gob.ar/handle/123456789/2247
10.1007/s00430-013-0325-y
identifier_str_mv 1432-1831
10.1007/s00430-013-0325-y
url http://sgc.anlis.gob.ar/handle/123456789/2247
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medical microbiology and immunology
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Medical Microbiology and Immunology 2014;203(3):145-154
pdf
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.623145

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