Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis

• Autores: Celeste, B. J.; Angel, Sergio O.; Castro, G. M.; Gidlund, M.; Goto, H.
• Año de publicación: 2004
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Brazilian Journal of Medical and Biological Research 2004;37(1):1591-1593
• Materia: Leishmania infantum; Ensayo de Inmunoadsorción Enzimática; Pruebas Serológicas; Enfermedad de Chagas
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Celeste, B. J. Instituto de Medicina Tropical de São Paulo. Laboratório de Soroepidemiologia e Imunobiologia. Departamentos de Dermatologia; Brasil.
Fil: Angel, Sergio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Castro, G. M. Instituto de Medicina Tropical de São Paulo. Laboratório de Soroepidemiologia e Imunobiologia. Departamentos de Dermatologia; Brasil.
Fil: Gidlund, M. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia, Laboratório de Imunofisiologia; Brasil.
Fil: Goto, H. Instituto de Medicina Tropical de São Paulo. Laboratório de Soroepidemiologia e Imunobiologia. Departamentos de Dermatologia; Brasil.
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as anti-Leishmania total antigen antibodies. When cross-reactivity was tested, chronic Chagas' disease patients (N = 10) did not show any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.