Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host

Autores
Fernandez-Brando, Romina J; Miliwebsky, Elizabeth; Mejías, M P; Baschkier, Ariela; Panek, C A; Abrey-Recalde, M J; Cabrera, Gabriel; Ramos, María Victoria; Rivas, Marta; Palermo, M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Mejías, M P. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, M. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Haemolytic uraemic syndrome (HUS) is a rare but life-threatening complication of Shiga toxin (Stx)-producing Escherichia coli (STEC) infections, characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. Although the main infection route is the consumption of contaminated food or water, person-to-person transmission has been suggested in several situations. Moreover, epidemiological data indicate that the horizontal transmission of several pathogens, including STEC, among individuals of the same species requires significantly lower doses than those used in animal models infected with laboratory-cultured bacteria. Thus, the aim of this study was to evaluate whether the passage of a clinically isolated STEC strain through the gastrointestinal tract of mice affects its pathogenicity in mice. To test this, weaned mice were orally inoculated by gavage with either an E. coli O157:H7 isolate from an HUS patient, or the same strain recovered from stools after one or two successive passages through the gastrointestinal tract of the mice. We show that stool-recovered strains are able to induce a more generalized and persistent colonization than the parent strain. Furthermore, a 10(4)-fold-reduced inoculum of the stool-recovered strains still causes gut colonization and mouse mortality, which are not observed with the parent strain. These results indicate an increased pathogenicity in stool-recovered strains that may be associated with an increased ability to colonize the mouse intestine.
Fuente
Journal of medical microbiology 2012; 61(Pt 6):852-859.
Materia
Toxina Shiga
Escherichia coli
Trombocitopenia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/2092
Acceder
id SGCANLIS_117138dc78535f2170c87bf0b85aaf4f
oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/2092
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same hostFernandez-Brando, Romina JMiliwebsky, ElizabethMejías, M PBaschkier, ArielaPanek, C AAbrey-Recalde, M JCabrera, GabrielRamos, María VictoriaRivas, MartaPalermo, M.Toxina ShigaEscherichia coliTrombocitopeniaFil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Mejías, M P. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Palermo, M. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.Haemolytic uraemic syndrome (HUS) is a rare but life-threatening complication of Shiga toxin (Stx)-producing Escherichia coli (STEC) infections, characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. Although the main infection route is the consumption of contaminated food or water, person-to-person transmission has been suggested in several situations. Moreover, epidemiological data indicate that the horizontal transmission of several pathogens, including STEC, among individuals of the same species requires significantly lower doses than those used in animal models infected with laboratory-cultured bacteria. Thus, the aim of this study was to evaluate whether the passage of a clinically isolated STEC strain through the gastrointestinal tract of mice affects its pathogenicity in mice. To test this, weaned mice were orally inoculated by gavage with either an E. coli O157:H7 isolate from an HUS patient, or the same strain recovered from stools after one or two successive passages through the gastrointestinal tract of the mice. We show that stool-recovered strains are able to induce a more generalized and persistent colonization than the parent strain. Furthermore, a 10(4)-fold-reduced inoculum of the stool-recovered strains still causes gut colonization and mouse mortality, which are not observed with the parent strain. These results indicate an increased pathogenicity in stool-recovered strains that may be associated with an increased ability to colonize the mouse intestine.Microbiology Society2012-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1473-5644https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.041251-0#tab2http://sgc.anlis.gob.ar/handle/123456789/209210.1099/jmm.0.041251-0Journal of medical microbiology 2012; 61(Pt 6):852-859.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of medical microbiologyenginfo:eu-repo/semantics/openAccess2025-09-04T11:18:11Zoai:sgc.anlis.gob.ar:Publications/123456789/2092Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:18:11.999Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
title Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
spellingShingle Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
Fernandez-Brando, Romina J
Toxina Shiga
Escherichia coli
Trombocitopenia
title_short Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
title_full Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
title_fullStr Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
title_full_unstemmed Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
title_sort Shiga toxin-producing Escherichia coli O157 : H7 shows an increased pathogenicity in mice after the passage through the gastrointestinal tract of the same host
dc.creator.none.fl_str_mv Fernandez-Brando, Romina J
Miliwebsky, Elizabeth
Mejías, M P
Baschkier, Ariela
Panek, C A
Abrey-Recalde, M J
Cabrera, Gabriel
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author Fernandez-Brando, Romina J
author_facet Fernandez-Brando, Romina J
Miliwebsky, Elizabeth
Mejías, M P
Baschkier, Ariela
Panek, C A
Abrey-Recalde, M J
Cabrera, Gabriel
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author_role author
author2 Miliwebsky, Elizabeth
Mejías, M P
Baschkier, Ariela
Panek, C A
Abrey-Recalde, M J
Cabrera, Gabriel
Ramos, María Victoria
Rivas, Marta
Palermo, M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxina Shiga
Escherichia coli
Trombocitopenia
topic Toxina Shiga
Escherichia coli
Trombocitopenia
dc.description.none.fl_txt_mv Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Mejías, M P. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Panek, C A. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Abrey-Recalde, M J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Cabrera, Gabriel. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Ramos, María Victoria. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, M. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
Haemolytic uraemic syndrome (HUS) is a rare but life-threatening complication of Shiga toxin (Stx)-producing Escherichia coli (STEC) infections, characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. Although the main infection route is the consumption of contaminated food or water, person-to-person transmission has been suggested in several situations. Moreover, epidemiological data indicate that the horizontal transmission of several pathogens, including STEC, among individuals of the same species requires significantly lower doses than those used in animal models infected with laboratory-cultured bacteria. Thus, the aim of this study was to evaluate whether the passage of a clinically isolated STEC strain through the gastrointestinal tract of mice affects its pathogenicity in mice. To test this, weaned mice were orally inoculated by gavage with either an E. coli O157:H7 isolate from an HUS patient, or the same strain recovered from stools after one or two successive passages through the gastrointestinal tract of the mice. We show that stool-recovered strains are able to induce a more generalized and persistent colonization than the parent strain. Furthermore, a 10(4)-fold-reduced inoculum of the stool-recovered strains still causes gut colonization and mouse mortality, which are not observed with the parent strain. These results indicate an increased pathogenicity in stool-recovered strains that may be associated with an increased ability to colonize the mouse intestine.
description Fil: Fernandez-Brando, Romina J. División Inmunología, Instituto de Medicina Experimental (CONICET), Academia Nacional de Medicina, Buenos Aires; Argentina.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1473-5644
https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.041251-0#tab2
http://sgc.anlis.gob.ar/handle/123456789/2092
10.1099/jmm.0.041251-0
identifier_str_mv 1473-5644
10.1099/jmm.0.041251-0
url https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.041251-0#tab2
http://sgc.anlis.gob.ar/handle/123456789/2092
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of medical microbiology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv Journal of medical microbiology 2012; 61(Pt 6):852-859.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.623145

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