Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity

• Autores: Periolo, Natalia; Avaro, Martín; Czech, A; Russo, Mara; Benedetti, Estefanía; Pontoriero, Andrea; Campos, Ana; Martínez-Peralta, Liliana; Baumeister, Elsa
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of clinical virology 2015;64:52-58
• Materia: Quimiocinas; Citocinas; Orthomyxoviridae; Mujeres Embarazadas
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Periolo, Natalia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Avaro, Martín. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Czech, A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Russo, Mara. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Benedetti, Estefanía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Fil: Martínez-Peralta, Liliana. Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA CONICET). Instituto de Microbiología y Parasitología Médica; Argentina.
Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. Servicio de Virosis Respiratoria. Laboratorio Nacional de Referencia de Influenza y Virus Respiratorios. National Influenza Centre PAHO/WHO; Argentina.
Background During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.