Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity

Autores
Periolo, Natalia; Avaro, M.; Czech, A.; Russo, M.; Benedetti, E.; Pontoriero, A.; Campos, A.; Martinez Peralta, Liliana A.; Baumeister, Elsa
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives: The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design: This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results: Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions: Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.
Fil: Periolo, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Avaro, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Czech, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Russo, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Benedetti, E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Pontoriero, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Campos, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Martinez Peralta, Liliana A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Baumeister, Elsa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Materia
A(H1n1)Pdm09
Chemokines
Cytokines
Influenza Virus
Pregnant Women
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38309
Acceder
id CONICETDig_8fa5168a564642d8bab10ad683895565
oai_identifier_str oai:ri.conicet.gov.ar:11336/38309
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severityPeriolo, NataliaAvaro, M.Czech, A.Russo, M.Benedetti, E.Pontoriero, A.Campos, A.Martinez Peralta, Liliana A.Baumeister, ElsaA(H1n1)Pdm09ChemokinesCytokinesInfluenza VirusPregnant Womenhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives: The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design: This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results: Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions: Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.Fil: Periolo, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Avaro, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Czech, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Russo, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Benedetti, E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Pontoriero, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Campos, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaFil: Martinez Peralta, Liliana A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Baumeister, Elsa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; ArgentinaElsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38309Periolo, Natalia; Avaro, M.; Czech, A.; Russo, M.; Benedetti, E.; et al.; Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity; Elsevier Science; Journal of Clinical Virology; 64; 3-2015; 52-581386-6532CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcv.2015.01.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1386653215000219info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:05Zoai:ri.conicet.gov.ar:11336/38309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:05.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
title Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
spellingShingle Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
Periolo, Natalia
A(H1n1)Pdm09
Chemokines
Cytokines
Influenza Virus
Pregnant Women
title_short Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
title_full Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
title_fullStr Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
title_full_unstemmed Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
title_sort Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity
dc.creator.none.fl_str_mv Periolo, Natalia
Avaro, M.
Czech, A.
Russo, M.
Benedetti, E.
Pontoriero, A.
Campos, A.
Martinez Peralta, Liliana A.
Baumeister, Elsa
author Periolo, Natalia
author_facet Periolo, Natalia
Avaro, M.
Czech, A.
Russo, M.
Benedetti, E.
Pontoriero, A.
Campos, A.
Martinez Peralta, Liliana A.
Baumeister, Elsa
author_role author
author2 Avaro, M.
Czech, A.
Russo, M.
Benedetti, E.
Pontoriero, A.
Campos, A.
Martinez Peralta, Liliana A.
Baumeister, Elsa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv A(H1n1)Pdm09
Chemokines
Cytokines
Influenza Virus
Pregnant Women
topic A(H1n1)Pdm09
Chemokines
Cytokines
Influenza Virus
Pregnant Women
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives: The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design: This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results: Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions: Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.
Fil: Periolo, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Avaro, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Czech, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Russo, M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Benedetti, E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Pontoriero, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Campos, A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
Fil: Martinez Peralta, Liliana A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Baumeister, Elsa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio E Instituto de Salud ; Argentina
description Background: During pregnancy, immunological and hormonal alterations place women at increased risk for influenza-related severe illnesses including hospitalization and death. Although A(H1N1) pdm09 infection resulted in increased disease severity in pregnant women, the precise mechanisms responsible for this risk have yet to be established. Objectives: The present study was aimed to investigate the role of host chemokines and cytokine profiles in A(H1N1) pdm09 infection regarding disease severity in pregnant women. Study design: This retrospective survey examined 41 pregnant women with confirmed A(H1N1) pdm09 infection. Of them, 12 died (D), 29 survived (S), and 17 remained uninfected and served as controls (C). Antiviral response was evaluated for IFN-β expression and gene expression profiles of cytokines (TNF-α, IL-6, IL-12, TGF-β) and chemokines (IL-8, RANTES, MCP-1, IP-10), and the viral Matrix (M1) gene was quantified and normalized using the housekeeping gene product β-actin mRNA. Results: Higher IL-8 and TNF-α mRNA expression were found in D and S compared with C, while IL-6 showed higher expression in D. Interestingly, these results were associated with a decrease in the anti-inflammatory response of TGF-β mRNA and IFN-β. These alterations would lead to an imbalance in the immune response of those patients. Conclusions: Pregnancy-related reductions in IFN-β and TGF-β expression levels and elevated levels of pro-inflammatory cytokines could explain the increased severity of infection and death of pregnant women. These findings may help improve the understanding of the high susceptibility and disease severity to influenza virus infection during pregnancy.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38309
Periolo, Natalia; Avaro, M.; Czech, A.; Russo, M.; Benedetti, E.; et al.; Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity; Elsevier Science; Journal of Clinical Virology; 64; 3-2015; 52-58
1386-6532
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38309
identifier_str_mv Periolo, Natalia; Avaro, M.; Czech, A.; Russo, M.; Benedetti, E.; et al.; Pregnant women infected with pandemic influenza A(H1N1)pdm09 virus showed differential immune response correlated with disease severity; Elsevier Science; Journal of Clinical Virology; 64; 3-2015; 52-58
1386-6532
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcv.2015.01.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1386653215000219
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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