Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)

Autores
Lounnas, Manon; Vázquez, Antonio A.; Alda, María del Pilar; Sartori, Kevin; Pointier, Jean Pierre; David, Patrice; Hurtrez Boussès, Sylvie
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
Centro de Estudios Parasitológicos y de Vectores
Facultad de Ciencias Naturales y Museo
Materia
Ciencias Naturales
Galba cubensis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87185
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)Lounnas, ManonVázquez, Antonio A.Alda, María del PilarSartori, KevinPointier, Jean PierreDavid, PatriceHurtrez Boussès, SylvieCiencias NaturalesGalba cubensisThe freshwater snail <i>Galba cubensis</i> (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of <i>Fasciola hepatica</i> - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in <i>G. cubensis</i> to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of <i>G. cubensis</i> from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: <i>G. truncatula</i>, <i>G. viator</i> and <i>G. neotropica</i>. We found that <i>G. cubensis</i> has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in <i>G. truncatula</i> and <i>G. viator</i>, respectively, and that all 15 loci amplified in <i>G. neotropica</i>. This last finding suggests a close relatedness between <i>G. cubensis</i> and <i>G. neotropica</i>, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Naturales y Museo2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf63-68http://sedici.unlp.edu.ar/handle/10915/87185enginfo:eu-repo/semantics/altIdentifier/issn/0260-1230info:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyw041info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:09:13Zoai:sedici.unlp.edu.ar:10915/87185Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:09:13.279SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
spellingShingle Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
Lounnas, Manon
Ciencias Naturales
Galba cubensis
title_short Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_full Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_fullStr Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_full_unstemmed Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
title_sort Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail <i>Galba cubensis</i> (Lymnaeidae)
dc.creator.none.fl_str_mv Lounnas, Manon
Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author Lounnas, Manon
author_facet Lounnas, Manon
Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author_role author
author2 Vázquez, Antonio A.
Alda, María del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Galba cubensis
topic Ciencias Naturales
Galba cubensis
dc.description.none.fl_txt_mv The freshwater snail <i>Galba cubensis</i> (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of <i>Fasciola hepatica</i> - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in <i>G. cubensis</i> to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of <i>G. cubensis</i> from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: <i>G. truncatula</i>, <i>G. viator</i> and <i>G. neotropica</i>. We found that <i>G. cubensis</i> has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in <i>G. truncatula</i> and <i>G. viator</i>, respectively, and that all 15 loci amplified in <i>G. neotropica</i>. This last finding suggests a close relatedness between <i>G. cubensis</i> and <i>G. neotropica</i>, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
Centro de Estudios Parasitológicos y de Vectores
Facultad de Ciencias Naturales y Museo
description The freshwater snail <i>Galba cubensis</i> (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of <i>Fasciola hepatica</i> - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in <i>G. cubensis</i> to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of <i>G. cubensis</i> from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: <i>G. truncatula</i>, <i>G. viator</i> and <i>G. neotropica</i>. We found that <i>G. cubensis</i> has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in <i>G. truncatula</i> and <i>G. viator</i>, respectively, and that all 15 loci amplified in <i>G. neotropica</i>. This last finding suggests a close relatedness between <i>G. cubensis</i> and <i>G. neotropica</i>, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87185
url http://sedici.unlp.edu.ar/handle/10915/87185
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0260-1230
info:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyw041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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