Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)

Autores
Lounnas, Manon; Vázquez, Antonio A.; Alda, Maria del Pilar; Sartori, Kevin; Pointier, Jean Pierre; David, Patrice; Hurtrez Boussès, Sylvie
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
Fil: Lounnas, Manon. Université de Montpellier; Francia
Fil: Vázquez, Antonio A.. Université de Montpellier; Francia. Instituto de Medicina Tropical Pedro Kourí; Francia
Fil: Alda, Maria del Pilar. Université de Montpellier; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Sartori, Kevin. Université de Montpellier; Francia
Fil: Pointier, Jean Pierre. Université de Perpignan; Francia
Fil: David, Patrice. Centre d'Ecologie Fonctionnelle et Evolutive; Francia
Fil: Hurtrez Boussès, Sylvie. Université de Montpellier; Francia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62836
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62836
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)Lounnas, ManonVázquez, Antonio A.Alda, Maria del PilarSartori, KevinPointier, Jean PierreDavid, PatriceHurtrez Boussès, Sylviehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.Fil: Lounnas, Manon. Université de Montpellier; FranciaFil: Vázquez, Antonio A.. Université de Montpellier; Francia. Instituto de Medicina Tropical Pedro Kourí; FranciaFil: Alda, Maria del Pilar. Université de Montpellier; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Sartori, Kevin. Université de Montpellier; FranciaFil: Pointier, Jean Pierre. Université de Perpignan; FranciaFil: David, Patrice. Centre d'Ecologie Fonctionnelle et Evolutive; FranciaFil: Hurtrez Boussès, Sylvie. Université de Montpellier; FranciaOxford University Press2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62836Lounnas, Manon; Vázquez, Antonio A.; Alda, Maria del Pilar; Sartori, Kevin; Pointier, Jean Pierre; et al.; Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae); Oxford University Press; Journal of Molluscan Studies; 83; 1; 12-2016; 63-680260-1230CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyw041info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mollus/article/83/1/63/2605921info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:39:58Zoai:ri.conicet.gov.ar:11336/62836instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:39:58.873CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
title Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
spellingShingle Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
Lounnas, Manon
title_short Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
title_full Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
title_fullStr Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
title_full_unstemmed Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
title_sort Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae)
dc.creator.none.fl_str_mv Lounnas, Manon
Vázquez, Antonio A.
Alda, Maria del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author Lounnas, Manon
author_facet Lounnas, Manon
Vázquez, Antonio A.
Alda, Maria del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author_role author
author2 Vázquez, Antonio A.
Alda, Maria del Pilar
Sartori, Kevin
Pointier, Jean Pierre
David, Patrice
Hurtrez Boussès, Sylvie
author2_role author
author
author
author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
Fil: Lounnas, Manon. Université de Montpellier; Francia
Fil: Vázquez, Antonio A.. Université de Montpellier; Francia. Instituto de Medicina Tropical Pedro Kourí; Francia
Fil: Alda, Maria del Pilar. Université de Montpellier; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Sartori, Kevin. Université de Montpellier; Francia
Fil: Pointier, Jean Pierre. Université de Perpignan; Francia
Fil: David, Patrice. Centre d'Ecologie Fonctionnelle et Evolutive; Francia
Fil: Hurtrez Boussès, Sylvie. Université de Montpellier; Francia
description The freshwater snail Galba cubensis (Pfeiffer, 1839) has a large distribution in the Americas. Despite being an intermediate host of Fasciola hepatica - the trematode causing fasciolosis in livestock and humans - its population genetics have never been studied. We isolated and characterized 15 microsatellite loci in G. cubensis to evaluate its genetic diversity, population-genetic structure and mating system. We tested the microsatellite loci in 359 individuals from 13 populations of G. cubensis from Cuba, Guadeloupe, Martinique, Puerto Rico, Venezuela, Colombia and Ecuador. We also tested cross-amplification in three closely related species: G. truncatula, G. viator and G. neotropica. We found that G. cubensis has a similar population structure to other selfing lymnaeids that live in temporary habitats: low genetic diversity, large departure from Hardy-Weinberg equilibrium, marked population structure and high selfing rate. We found that seven and six loci amplified in G. truncatula and G. viator, respectively, and that all 15 loci amplified in G. neotropica. This last finding suggests a close relatedness between G. cubensis and G. neotropica, probably being conspecific and synonymous. This new set of microsatellite markers will be a useful tool to study the genetic diversity of this snail species across a large geographical range and, consequently, to understand the emergence and re-emergence of fasciolosis in the Americas.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62836
Lounnas, Manon; Vázquez, Antonio A.; Alda, Maria del Pilar; Sartori, Kevin; Pointier, Jean Pierre; et al.; Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae); Oxford University Press; Journal of Molluscan Studies; 83; 1; 12-2016; 63-68
0260-1230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62836
identifier_str_mv Lounnas, Manon; Vázquez, Antonio A.; Alda, Maria del Pilar; Sartori, Kevin; Pointier, Jean Pierre; et al.; Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae); Oxford University Press; Journal of Molluscan Studies; 83; 1; 12-2016; 63-68
0260-1230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyw041
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mollus/article/83/1/63/2605921
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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