Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxi...

Autores
Cadavid Vargas, Juan Fernando; Arnal, Pablo Maximiliano; Mojica Sepúlveda, Ruth Dary; Rizzo, Andrea Paula; Soria, Delia Beatriz; Di Virgilio, Ana Laura
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ideal drugs to cure cancer leave normal cells unharmed while selectively turning tumor cells unviable. Several copper complexes have been able to selectively slow down tumor proliferation. We hypothesized that Cu(smz)₂(bipy)H₂O (1)—a copper-complex that has two ligands capable of interacting with DNA—would outperform Cu(smz)₂(OH₂)₂H₂O (2), and also that supporting 1 on mesoporous silica spheres would decrease even further tumor cell viability in vitro. After exposing osteosarcoma cells (MG-63) and normal phenotype cells of bone origin (MC3T3-E1) to either complex, we studied their toxic effect and mechanisms of action. We determined cell viability (MTT assay) and quantified formation of reactive oxygen species (oxidation of DHR-123 to rhodamine). Moreover, we assessed genotoxicity from (i) formation of micronucleus (MN assay) and (ii) damage of DNA (Comet assay). After the exposure of 1 supported on silica spheres, we tested cell viability. Our results confirm our hypotheses: inhibition of tumor cells follows: supported 1 > dissolved 1 > 2. Future work that enhances the load of the complex exclusively in mesopores may improve the ability of 1 to further inhibit tumor cell viability.
Facultad de Ciencias Exactas
Centro de Química Inorgánica
Centro de Tecnología de Recursos Minerales y Cerámica
Materia
Ciencias Exactas
Química
Antitumor effect
Copper(II) complexes
Cytotoxicity
Genotoxicity
Mesoporous silica microspheres
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/128174
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/128174
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effectsCadavid Vargas, Juan FernandoArnal, Pablo MaximilianoMojica Sepúlveda, Ruth DaryRizzo, Andrea PaulaSoria, Delia BeatrizDi Virgilio, Ana LauraCiencias ExactasQuímicaAntitumor effectCopper(II) complexesCytotoxicityGenotoxicityMesoporous silica microspheresIdeal drugs to cure cancer leave normal cells unharmed while selectively turning tumor cells unviable. Several copper complexes have been able to selectively slow down tumor proliferation. We hypothesized that Cu(smz)₂(bipy)H₂O (1)—a copper-complex that has two ligands capable of interacting with DNA—would outperform Cu(smz)₂(OH₂)₂H₂O (2), and also that supporting 1 on mesoporous silica spheres would decrease even further tumor cell viability in vitro. After exposing osteosarcoma cells (MG-63) and normal phenotype cells of bone origin (MC3T3-E1) to either complex, we studied their toxic effect and mechanisms of action. We determined cell viability (MTT assay) and quantified formation of reactive oxygen species (oxidation of DHR-123 to rhodamine). Moreover, we assessed genotoxicity from (i) formation of micronucleus (MN assay) and (ii) damage of DNA (Comet assay). After the exposure of 1 supported on silica spheres, we tested cell viability. Our results confirm our hypotheses: inhibition of tumor cells follows: supported 1 > dissolved 1 > 2. Future work that enhances the load of the complex exclusively in mesopores may improve the ability of 1 to further inhibit tumor cell viability.Facultad de Ciencias ExactasCentro de Química InorgánicaCentro de Tecnología de Recursos Minerales y Cerámica2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf21-32http://sedici.unlp.edu.ar/handle/10915/128174enginfo:eu-repo/semantics/altIdentifier/issn/1572-8773info:eu-repo/semantics/altIdentifier/issn/0966-0844info:eu-repo/semantics/altIdentifier/pmid/30334122info:eu-repo/semantics/altIdentifier/doi/10.1007/s10534-018-0154-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:11:52Zoai:sedici.unlp.edu.ar:10915/128174Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:11:52.652SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
title Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
spellingShingle Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
Cadavid Vargas, Juan Fernando
Ciencias Exactas
Química
Antitumor effect
Copper(II) complexes
Cytotoxicity
Genotoxicity
Mesoporous silica microspheres
title_short Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
title_full Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
title_fullStr Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
title_full_unstemmed Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
title_sort Copper complex with sulfamethazine and 2,2'-bipyridine supported on mesoporous silica microspheres improves its antitumor action toward human osteosarcoma cells: cyto- and genotoxic effects
dc.creator.none.fl_str_mv Cadavid Vargas, Juan Fernando
Arnal, Pablo Maximiliano
Mojica Sepúlveda, Ruth Dary
Rizzo, Andrea Paula
Soria, Delia Beatriz
Di Virgilio, Ana Laura
author Cadavid Vargas, Juan Fernando
author_facet Cadavid Vargas, Juan Fernando
Arnal, Pablo Maximiliano
Mojica Sepúlveda, Ruth Dary
Rizzo, Andrea Paula
Soria, Delia Beatriz
Di Virgilio, Ana Laura
author_role author
author2 Arnal, Pablo Maximiliano
Mojica Sepúlveda, Ruth Dary
Rizzo, Andrea Paula
Soria, Delia Beatriz
Di Virgilio, Ana Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Antitumor effect
Copper(II) complexes
Cytotoxicity
Genotoxicity
Mesoporous silica microspheres
topic Ciencias Exactas
Química
Antitumor effect
Copper(II) complexes
Cytotoxicity
Genotoxicity
Mesoporous silica microspheres
dc.description.none.fl_txt_mv Ideal drugs to cure cancer leave normal cells unharmed while selectively turning tumor cells unviable. Several copper complexes have been able to selectively slow down tumor proliferation. We hypothesized that Cu(smz)₂(bipy)H₂O (1)—a copper-complex that has two ligands capable of interacting with DNA—would outperform Cu(smz)₂(OH₂)₂H₂O (2), and also that supporting 1 on mesoporous silica spheres would decrease even further tumor cell viability in vitro. After exposing osteosarcoma cells (MG-63) and normal phenotype cells of bone origin (MC3T3-E1) to either complex, we studied their toxic effect and mechanisms of action. We determined cell viability (MTT assay) and quantified formation of reactive oxygen species (oxidation of DHR-123 to rhodamine). Moreover, we assessed genotoxicity from (i) formation of micronucleus (MN assay) and (ii) damage of DNA (Comet assay). After the exposure of 1 supported on silica spheres, we tested cell viability. Our results confirm our hypotheses: inhibition of tumor cells follows: supported 1 > dissolved 1 > 2. Future work that enhances the load of the complex exclusively in mesopores may improve the ability of 1 to further inhibit tumor cell viability.
Facultad de Ciencias Exactas
Centro de Química Inorgánica
Centro de Tecnología de Recursos Minerales y Cerámica
description Ideal drugs to cure cancer leave normal cells unharmed while selectively turning tumor cells unviable. Several copper complexes have been able to selectively slow down tumor proliferation. We hypothesized that Cu(smz)₂(bipy)H₂O (1)—a copper-complex that has two ligands capable of interacting with DNA—would outperform Cu(smz)₂(OH₂)₂H₂O (2), and also that supporting 1 on mesoporous silica spheres would decrease even further tumor cell viability in vitro. After exposing osteosarcoma cells (MG-63) and normal phenotype cells of bone origin (MC3T3-E1) to either complex, we studied their toxic effect and mechanisms of action. We determined cell viability (MTT assay) and quantified formation of reactive oxygen species (oxidation of DHR-123 to rhodamine). Moreover, we assessed genotoxicity from (i) formation of micronucleus (MN assay) and (ii) damage of DNA (Comet assay). After the exposure of 1 supported on silica spheres, we tested cell viability. Our results confirm our hypotheses: inhibition of tumor cells follows: supported 1 > dissolved 1 > 2. Future work that enhances the load of the complex exclusively in mesopores may improve the ability of 1 to further inhibit tumor cell viability.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/128174
url http://sedici.unlp.edu.ar/handle/10915/128174
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1572-8773
info:eu-repo/semantics/altIdentifier/issn/0966-0844
info:eu-repo/semantics/altIdentifier/pmid/30334122
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10534-018-0154-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
21-32
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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