Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex

Autores
Martini, Nancy; Parente, Juliana Elena; Toledo, María Eugenia; Escudero, Graciela Estela; Laino, Carlos Horacio; Piro, Oscar Enrique; Echeverría, Gustavo Alberto; Williams, Patricia Ana María; Ferrer, Evelina Gloria
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Magnesium is an essential element related with biochemistry of the brain and different types of depression have been associated with its deficiency. Methods: The structure of a novel magnesium bis(DL-pyroglutamate) (Mg(DL-pGlu)₂) was elucidated by X-ray crystallography. Wistar rats were used in the in vivo experiments. The antidepressant-like effect was assessed by the forced swim test (FST) and the antinociceptive activity was evaluated using hot plate test. In both, non-specific effects were evaluated by the open field test. Anti-thyroid activity was examined using Lang’s method. Albumin binding behavior was evaluated by 3D fluorescence spectroscopy. Results: For the Mg(DL-pGlu)₂ complex (30 mg/kg), the FST test on Wistar rats revealed a decrease of 22% in the immobility time and an increment of 106% in the swimming time. The compound alters neither the locomotor activity nor the body weight after chronic administration. At the same dose, it showed antinociceptive activity, increasing the response latency. It blocks iodination reactions generating a charge transfer complex with iodine hence indicating anti-thyroid activity (Kc = 45366.5 ± 29 M⁻¹). Albumin 3D fluorescence spectroscopy experiments showed intensity increase of peak A and decrease of peak B. Conclusions: The results showed that the new compound produced a lowering of the immobility time and an increment of the swimming ability of the rats. The compound is able to increase the response latency in 70.0%, to capture iodine (anti-thyroid activity) and to interact with albumin through covalent type of interaction of the free NH groups.
Facultad de Ciencias Exactas
Centro de Química Inorgánica
Instituto de Física La Plata
Materia
Ciencias Exactas
Química
DL-pyroglutamic acid
Magnesium
Antidepressant activity
Anti-thyroid activity
Antinociceptive activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131021
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/131021
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complexMartini, NancyParente, Juliana ElenaToledo, María EugeniaEscudero, Graciela EstelaLaino, Carlos HoracioPiro, Oscar EnriqueEcheverría, Gustavo AlbertoWilliams, Patricia Ana MaríaFerrer, Evelina GloriaCiencias ExactasQuímicaDL-pyroglutamic acidMagnesiumAntidepressant activityAnti-thyroid activityAntinociceptive activityBackground: Magnesium is an essential element related with biochemistry of the brain and different types of depression have been associated with its deficiency. Methods: The structure of a novel magnesium bis(DL-pyroglutamate) (Mg(DL-pGlu)₂) was elucidated by X-ray crystallography. Wistar rats were used in the </i>in vivo</i> experiments. The antidepressant-like effect was assessed by the forced swim test (FST) and the antinociceptive activity was evaluated using hot plate test. In both, non-specific effects were evaluated by the open field test. Anti-thyroid activity was examined using Lang’s method. Albumin binding behavior was evaluated by 3D fluorescence spectroscopy. Results: For the Mg(DL-pGlu)₂ complex (30 mg/kg), the FST test on Wistar rats revealed a decrease of 22% in the immobility time and an increment of 106% in the swimming time. The compound alters neither the locomotor activity nor the body weight after chronic administration. At the same dose, it showed antinociceptive activity, increasing the response latency. It blocks iodination reactions generating a charge transfer complex with iodine hence indicating anti-thyroid activity (K<sub>c</sub> = 45366.5 ± 29 M⁻¹). Albumin 3D fluorescence spectroscopy experiments showed intensity increase of peak A and decrease of peak B. Conclusions: The results showed that the new compound produced a lowering of the immobility time and an increment of the swimming ability of the rats. The compound is able to increase the response latency in 70.0%, to capture iodine (anti-thyroid activity) and to interact with albumin through covalent type of interaction of the free NH groups.Facultad de Ciencias ExactasCentro de Química InorgánicaInstituto de Física La Plata2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1273-1280http://sedici.unlp.edu.ar/handle/10915/131021enginfo:eu-repo/semantics/altIdentifier/issn/1734-1140info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pharep.2019.07.009info:eu-repo/semantics/altIdentifier/pmid/31675673; 32002857info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:43Zoai:sedici.unlp.edu.ar:10915/131021Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:43.297SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
title Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
spellingShingle Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
Martini, Nancy
Ciencias Exactas
Química
DL-pyroglutamic acid
Magnesium
Antidepressant activity
Anti-thyroid activity
Antinociceptive activity
title_short Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
title_full Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
title_fullStr Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
title_full_unstemmed Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
title_sort Antidepressant and antinociceptive activities in animal models and <i>in vitro</i> assessment of the anti-thyroid activity of bis(DL-pyroglutamate)magnesium complex
dc.creator.none.fl_str_mv Martini, Nancy
Parente, Juliana Elena
Toledo, María Eugenia
Escudero, Graciela Estela
Laino, Carlos Horacio
Piro, Oscar Enrique
Echeverría, Gustavo Alberto
Williams, Patricia Ana María
Ferrer, Evelina Gloria
author Martini, Nancy
author_facet Martini, Nancy
Parente, Juliana Elena
Toledo, María Eugenia
Escudero, Graciela Estela
Laino, Carlos Horacio
Piro, Oscar Enrique
Echeverría, Gustavo Alberto
Williams, Patricia Ana María
Ferrer, Evelina Gloria
author_role author
author2 Parente, Juliana Elena
Toledo, María Eugenia
Escudero, Graciela Estela
Laino, Carlos Horacio
Piro, Oscar Enrique
Echeverría, Gustavo Alberto
Williams, Patricia Ana María
Ferrer, Evelina Gloria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
DL-pyroglutamic acid
Magnesium
Antidepressant activity
Anti-thyroid activity
Antinociceptive activity
topic Ciencias Exactas
Química
DL-pyroglutamic acid
Magnesium
Antidepressant activity
Anti-thyroid activity
Antinociceptive activity
dc.description.none.fl_txt_mv Background: Magnesium is an essential element related with biochemistry of the brain and different types of depression have been associated with its deficiency. Methods: The structure of a novel magnesium bis(DL-pyroglutamate) (Mg(DL-pGlu)₂) was elucidated by X-ray crystallography. Wistar rats were used in the </i>in vivo</i> experiments. The antidepressant-like effect was assessed by the forced swim test (FST) and the antinociceptive activity was evaluated using hot plate test. In both, non-specific effects were evaluated by the open field test. Anti-thyroid activity was examined using Lang’s method. Albumin binding behavior was evaluated by 3D fluorescence spectroscopy. Results: For the Mg(DL-pGlu)₂ complex (30 mg/kg), the FST test on Wistar rats revealed a decrease of 22% in the immobility time and an increment of 106% in the swimming time. The compound alters neither the locomotor activity nor the body weight after chronic administration. At the same dose, it showed antinociceptive activity, increasing the response latency. It blocks iodination reactions generating a charge transfer complex with iodine hence indicating anti-thyroid activity (K<sub>c</sub> = 45366.5 ± 29 M⁻¹). Albumin 3D fluorescence spectroscopy experiments showed intensity increase of peak A and decrease of peak B. Conclusions: The results showed that the new compound produced a lowering of the immobility time and an increment of the swimming ability of the rats. The compound is able to increase the response latency in 70.0%, to capture iodine (anti-thyroid activity) and to interact with albumin through covalent type of interaction of the free NH groups.
Facultad de Ciencias Exactas
Centro de Química Inorgánica
Instituto de Física La Plata
description Background: Magnesium is an essential element related with biochemistry of the brain and different types of depression have been associated with its deficiency. Methods: The structure of a novel magnesium bis(DL-pyroglutamate) (Mg(DL-pGlu)₂) was elucidated by X-ray crystallography. Wistar rats were used in the </i>in vivo</i> experiments. The antidepressant-like effect was assessed by the forced swim test (FST) and the antinociceptive activity was evaluated using hot plate test. In both, non-specific effects were evaluated by the open field test. Anti-thyroid activity was examined using Lang’s method. Albumin binding behavior was evaluated by 3D fluorescence spectroscopy. Results: For the Mg(DL-pGlu)₂ complex (30 mg/kg), the FST test on Wistar rats revealed a decrease of 22% in the immobility time and an increment of 106% in the swimming time. The compound alters neither the locomotor activity nor the body weight after chronic administration. At the same dose, it showed antinociceptive activity, increasing the response latency. It blocks iodination reactions generating a charge transfer complex with iodine hence indicating anti-thyroid activity (K<sub>c</sub> = 45366.5 ± 29 M⁻¹). Albumin 3D fluorescence spectroscopy experiments showed intensity increase of peak A and decrease of peak B. Conclusions: The results showed that the new compound produced a lowering of the immobility time and an increment of the swimming ability of the rats. The compound is able to increase the response latency in 70.0%, to capture iodine (anti-thyroid activity) and to interact with albumin through covalent type of interaction of the free NH groups.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131021
url http://sedici.unlp.edu.ar/handle/10915/131021
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1734-1140
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pharep.2019.07.009
info:eu-repo/semantics/altIdentifier/pmid/31675673; 32002857
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1273-1280
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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