Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells

Autores
Cortizo, Ana María; Besterman, J. M.; Leitner, P. P.; Chandrabose, K. A.
Año de publicación
1992
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated.
Facultad de Ciencias Médicas
Materia
Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/67401
Acceder
id SEDICI_ee5aa4251e898a9cb373ea860cb3c2af
oai_identifier_str oai:sedici.unlp.edu.ar:10915/67401
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cellsCortizo, Ana MaríaBesterman, J. M.Leitner, P. P.Chandrabose, K. A.Ciencias Médicasphospholipase A2, eicosanoids, Madin-Darby canine kidney cellsRiñónThe receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated.Facultad de Ciencias Médicas1992info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf357-371http://sedici.unlp.edu.ar/handle/10915/67401enginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/009069809290008Hinfo:eu-repo/semantics/altIdentifier/issn/0090-6980info:eu-repo/semantics/altIdentifier/hdl/11746/4499info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:51:10Zoai:sedici.unlp.edu.ar:10915/67401Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:51:10.982SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
spellingShingle Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
Cortizo, Ana María
Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
title_short Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_full Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_fullStr Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_full_unstemmed Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
title_sort Stimulated release of arachidonate and prostaglandins is vectorial in MDCK epithelial cells
dc.creator.none.fl_str_mv Cortizo, Ana María
Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
author Cortizo, Ana María
author_facet Cortizo, Ana María
Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
author_role author
author2 Besterman, J. M.
Leitner, P. P.
Chandrabose, K. A.
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
topic Ciencias Médicas
phospholipase A2, eicosanoids, Madin-Darby canine kidney cells
Riñón
dc.description.none.fl_txt_mv The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated.
Facultad de Ciencias Médicas
description The receptor mediated activation of phospholipase A2by appropriate ligands results in the synthesis and release of eicosanoids, a class of potent bioregulatory molecules. Madin-Darby canine kidney cells (MDCK) are polarized epithelial cells, with structurally and functionally distinct plasma membrane domains separated by tight junctions. Using MDCK cells grown in dual sided chambers, we show in this report, that a) the receptor mediated release of prostaglandins and arachidonate into the extracellular medium is predominantly unidirectional, b) the direction of release is agonist specific, and c) the magnitude of the response due to a given agonist is cell-domain specific. These characteristics, if operativein vivo, would contribute towards the optimal function of trans-cellular metabolism of eicosanoids already demonstrated.
publishDate 1992
dc.date.none.fl_str_mv 1992
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/67401
url http://sedici.unlp.edu.ar/handle/10915/67401
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/009069809290008H
info:eu-repo/semantics/altIdentifier/issn/0090-6980
info:eu-repo/semantics/altIdentifier/hdl/11746/4499
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
357-371
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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