Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
- Autores
- Scrochi, Mariela Rita; Zanuzzi, Carolina Natalia; Fuentealba, Nadia Analía; Nishida, Fabián; Bravi, María Emilia; Pacheco, María Emilia; Sguazza, Guillermo Hernán; Gimeno, Eduardo Juan; Portiansky, Enrique Leo; Muglia, Cecilia Isabel; Galosi, Cecilia Mónica; Barbeito, Claudio Gustavo
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle.
Facultad de Ciencias Veterinarias
Facultad de Ciencias Exactas
Instituto de Estudios Inmunológicos y Fisiopatológicos - Materia
-
Veterinaria
Ciencias Exactas
Apoptosis
equine dermis cells
equine herpes virus 1
Madin–Darby bovine kidney cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/108503
Ver los metadatos del registro completo
| id |
SEDICI_edb0e2c732048b9fb45862ec1b5e9ec5 |
|---|---|
| oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/108503 |
| network_acronym_str |
SEDICI |
| repository_id_str |
1329 |
| network_name_str |
SEDICI (UNLP) |
| spelling |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1Scrochi, Mariela RitaZanuzzi, Carolina NataliaFuentealba, Nadia AnalíaNishida, FabiánBravi, María EmiliaPacheco, María EmiliaSguazza, Guillermo HernánGimeno, Eduardo JuanPortiansky, Enrique LeoMuglia, Cecilia IsabelGalosi, Cecilia MónicaBarbeito, Claudio GustavoVeterinariaCiencias ExactasApoptosisequine dermis cellsequine herpes virus 1Madin–Darby bovine kidney cellsMany viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle.Facultad de Ciencias VeterinariasFacultad de Ciencias ExactasInstituto de Estudios Inmunológicos y Fisiopatológicos2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf560-568http://sedici.unlp.edu.ar/handle/10915/108503enginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10520295.2017.1359749info:eu-repo/semantics/altIdentifier/issn/1473-7760info:eu-repo/semantics/altIdentifier/doi/10.1080/10520295.2017.1359749info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-10T12:27:16Zoai:sedici.unlp.edu.ar:10915/108503Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-10 12:27:16.793SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| title |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| spellingShingle |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 Scrochi, Mariela Rita Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells |
| title_short |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| title_full |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| title_fullStr |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| title_full_unstemmed |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| title_sort |
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1 |
| dc.creator.none.fl_str_mv |
Scrochi, Mariela Rita Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo |
| author |
Scrochi, Mariela Rita |
| author_facet |
Scrochi, Mariela Rita Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo |
| author_role |
author |
| author2 |
Zanuzzi, Carolina Natalia Fuentealba, Nadia Analía Nishida, Fabián Bravi, María Emilia Pacheco, María Emilia Sguazza, Guillermo Hernán Gimeno, Eduardo Juan Portiansky, Enrique Leo Muglia, Cecilia Isabel Galosi, Cecilia Mónica Barbeito, Claudio Gustavo |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells |
| topic |
Veterinaria Ciencias Exactas Apoptosis equine dermis cells equine herpes virus 1 Madin–Darby bovine kidney cells |
| dc.description.none.fl_txt_mv |
Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle. Facultad de Ciencias Veterinarias Facultad de Ciencias Exactas Instituto de Estudios Inmunológicos y Fisiopatológicos |
| description |
Many viruses alter different stages of apoptosis of infected cells as a strategy for successful infection. Few studies have addressed mechanisms of equine herpesvirus 1 (EHV-1) strain-induced cell death. We investigated the effect of an abortigenic strain (AR8 strain) on heterologous Madin–Darby bovine kidney cells and homologous equine dermis (ED) cells cell lines. We compared morphologic and biochemical features of early and late apoptosis at different postinfection times. We investigated translocation of phosphatidylserine to the cell surface, nuclear fragmentation and changes in the cytoskeleton using flow cytometry and annexin V/propidium iodide staining, DNA laddering, terminal deoxynucleotidyl transferase UTP nick-end labeling assay and immunofluorescence staining of cytokeratin 18 cleavage. AR8 EVH-1 strain interfered with apoptosis in both cell lines, particularly during the middle stage of the replication cycle; this was more evident in ED cells. Although this antiapoptotic effect has been reported for other alpha herpesviruses, our findings may help elucidate how EHV-1 improves its infectivity during its cycle. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/108503 |
| url |
http://sedici.unlp.edu.ar/handle/10915/108503 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10520295.2017.1359749 info:eu-repo/semantics/altIdentifier/issn/1473-7760 info:eu-repo/semantics/altIdentifier/doi/10.1080/10520295.2017.1359749 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| dc.format.none.fl_str_mv |
application/pdf 560-568 |
| dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
| reponame_str |
SEDICI (UNLP) |
| collection |
SEDICI (UNLP) |
| instname_str |
Universidad Nacional de La Plata |
| instacron_str |
UNLP |
| institution |
UNLP |
| repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
| repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
| _version_ |
1842904336018964480 |
| score |
12.993085 |