Fate of Bacillus cereus within phagocytic cells

Autores
Rolny, Ivanna Sabrina; Racedo, Silvia María; Pérez, Pablo Fernando
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/98111

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network_name_str SEDICI (UNLP)
spelling Fate of Bacillus cereus within phagocytic cellsRolny, Ivanna SabrinaRacedo, Silvia MaríaPérez, Pablo FernandoBiologíaBacillus cereusEndocytic pathwayIntracellular traffickingLAMP1PhagocytosisVirulenceIn this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf170-177http://sedici.unlp.edu.ar/handle/10915/98111enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/57838info:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/view/144200info:eu-repo/semantics/altIdentifier/issn/1618-1905info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.300info:eu-repo/semantics/altIdentifier/hdl/11336/57838info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:15Zoai:sedici.unlp.edu.ar:10915/98111Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:16.039SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Fate of Bacillus cereus within phagocytic cells
title Fate of Bacillus cereus within phagocytic cells
spellingShingle Fate of Bacillus cereus within phagocytic cells
Rolny, Ivanna Sabrina
Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
title_short Fate of Bacillus cereus within phagocytic cells
title_full Fate of Bacillus cereus within phagocytic cells
title_fullStr Fate of Bacillus cereus within phagocytic cells
title_full_unstemmed Fate of Bacillus cereus within phagocytic cells
title_sort Fate of Bacillus cereus within phagocytic cells
dc.creator.none.fl_str_mv Rolny, Ivanna Sabrina
Racedo, Silvia María
Pérez, Pablo Fernando
author Rolny, Ivanna Sabrina
author_facet Rolny, Ivanna Sabrina
Racedo, Silvia María
Pérez, Pablo Fernando
author_role author
author2 Racedo, Silvia María
Pérez, Pablo Fernando
author2_role author
author
dc.subject.none.fl_str_mv Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
topic Biología
Bacillus cereus
Endocytic pathway
Intracellular trafficking
LAMP1
Phagocytosis
Virulence
dc.description.none.fl_txt_mv In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/98111
url http://sedici.unlp.edu.ar/handle/10915/98111
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/57838
info:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/view/144200
info:eu-repo/semantics/altIdentifier/issn/1618-1905
info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.300
info:eu-repo/semantics/altIdentifier/hdl/11336/57838
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
170-177
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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