Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets

Autores
Maiztegui, Bárbara; Villagarcía, Hernán Gonzalo; Roman, Carolina Lisi; Flores, Luis Emilio; Prieto, José María; Castro, María Cecilia; Massa, María Laura; Schinella, Guillermo Raúl; Francini, Flavio
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
“Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes.
Centro de Endocrinología Experimental y Aplicada
Materia
Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/160006
Acceder
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network_name_str SEDICI (UNLP)
spelling Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic isletsMaiztegui, BárbaraVillagarcía, Hernán GonzaloRoman, Carolina LisiFlores, Luis EmilioPrieto, José MaríaCastro, María CeciliaMassa, María LauraSchinella, Guillermo RaúlFrancini, FlavioCiencias MédicasYerba mateIlex paraguariensisinsulin secretioninsulin sensitivitydiabetes“Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes.Centro de Endocrinología Experimental y Aplicada2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160006enginfo:eu-repo/semantics/altIdentifier/issn/2223-7747info:eu-repo/semantics/altIdentifier/doi/10.3390/plants12142620info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:13:50Zoai:sedici.unlp.edu.ar:10915/160006Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:13:50.945SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
spellingShingle Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
Maiztegui, Bárbara
Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
title_short Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_full Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_fullStr Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_full_unstemmed Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
title_sort Dietary supplementation with yerba mate (Ilex paraguariensis) infusion increases IRS-1 and PI3K mRNA levels and enhances insulin sensitivity and secretion in rat pancreatic islets
dc.creator.none.fl_str_mv Maiztegui, Bárbara
Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
author Maiztegui, Bárbara
author_facet Maiztegui, Bárbara
Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
author_role author
author2 Villagarcía, Hernán Gonzalo
Roman, Carolina Lisi
Flores, Luis Emilio
Prieto, José María
Castro, María Cecilia
Massa, María Laura
Schinella, Guillermo Raúl
Francini, Flavio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
topic Ciencias Médicas
Yerba mate
Ilex paraguariensis
insulin secretion
insulin sensitivity
diabetes
dc.description.none.fl_txt_mv “Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes.
Centro de Endocrinología Experimental y Aplicada
description “Yerba mate” (YM), an aqueous extract of Ilex paraguariensis, has antioxidant, diuretic, cardio-protective and hypoglycaemic properties. Since its effect on the pancreatic islets remains unclear, we evaluated insulin sensitivity and glucose-stimulated insulin secretion (GSIS) in rats consuming YM or tap water (C) for 21 days. Glucose tolerance, glycemia, triglyceridemia, insulinemia, TBARS and FRAP serum levels were evaluated. GSIS and mRNA levels of insulin signaling pathway and inflammatory markers were measured in isolated pancreatic islets from both groups. In C rats, islets were incubated with YM extract or its phenolic components to measure GSIS. YM improved glucose tolerance, enhanced GSIS, increased FRAP plasma levels and islet mRNA levels of IRS-1 and PI3K (p110), and decreased TBARS plasma levels and islet gene expression of TNF-α and PAI-1. Islets from C rats incubated with 100 µg/mL dry YM extract, 1 µM chlorogenic acid, 0.1 and 1 µM rutin, 1 µM caffeic acid or 1 µM quercetin showed an increase in GSIS. Our results suggest that YM enhances glucose tolerance because of its positive effects on GSIS, oxidative stress rate and insulin sensitivity in rat islets, suggesting that long-term dietary supplementation with YM may improve glucose homeostasis in pre-diabetes or type 2 diabetes.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/160006
url http://sedici.unlp.edu.ar/handle/10915/160006
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2223-7747
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants12142620
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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