Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"

Autores
Castellani, Lucas Gabriel; Luchetti, Abril; Nilsson, Juliet Fernanda; Pérez Giménez, Julieta; Struck, Ben; Schlüter, Andreas; Pühler, Alfred; Niehaus, Karsten; Romero, David; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids.
Fil: Castellani, Lucas Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Luchetti, Abril. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina,
Fil: Luchetti, Abril. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nilsson, Juliet Fernanda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pérez Giménez, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Facultad de Ciencias Exactas
Consejo Nacional de Investigaciones Científicas y Técnicas
Materia
Ciencias Exactas
Rhizobium
plasmids
conjugation
gene regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140513
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140513
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"Castellani, Lucas GabrielLuchetti, AbrilNilsson, Juliet FernandaPérez Giménez, JulietaStruck, BenSchlüter, AndreasPühler, AlfredNiehaus, KarstenRomero, DavidPistorio, MarianoTorres Tejerizo, Gonzalo ArturoCiencias Exactashttps://purl.org/becyt/ford/1.6Rhizobiumplasmidsconjugationgene regulationRhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids.Fil: Castellani, Lucas Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Luchetti, Abril. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina,Fil: Luchetti, Abril. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Nilsson, Juliet Fernanda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pérez Giménez, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pistorio, Mariano. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFacultad de Ciencias ExactasConsejo Nacional de Investigaciones Científicas y Técnicas2022-08-08info:eu-repo/semantics/publishedVersionConjunto de datoshttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosinfo:eu-repo/semantics/dataSetapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/140513https://doi.org/10.35537/10915/140513enginfo:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.01949-22info:eu-repo/semantics/reference/hdl/10915/151452info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:35:39Zoai:sedici.unlp.edu.ar:10915/140513Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:35:39.343SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
spellingShingle Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
Castellani, Lucas Gabriel
Ciencias Exactas
Rhizobium
plasmids
conjugation
gene regulation
title_short Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_full Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_fullStr Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_full_unstemmed Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
title_sort Supplementary material for "RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids"
dc.creator.none.fl_str_mv Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author Castellani, Lucas Gabriel
author_facet Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_role author
author2 Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Rhizobium
plasmids
conjugation
gene regulation
topic Ciencias Exactas
Rhizobium
plasmids
conjugation
gene regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids.
Fil: Castellani, Lucas Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Luchetti, Abril. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina,
Fil: Luchetti, Abril. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nilsson, Juliet Fernanda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pérez Giménez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pérez Giménez, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Facultad de Ciencias Exactas
Consejo Nacional de Investigaciones Científicas y Técnicas
description Rhizobia are gram-negative bacteria able to establish a nitrogen-fixing symbiotic interaction with leguminous plants. Rhizobia usually harbor several plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes were identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms have the same function. These findings set up the basis for a new regulatory circuit of conjugative transfer of plasmids.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-08
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Conjunto de datos
http://purl.org/coar/resource_type/c_ddb1
info:ar-repo/semantics/conjuntoDeDatos
info:eu-repo/semantics/dataSet
status_str publishedVersion
format dataSet
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140513
https://doi.org/10.35537/10915/140513
url http://sedici.unlp.edu.ar/handle/10915/140513
https://doi.org/10.35537/10915/140513
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.01949-22
info:eu-repo/semantics/reference/hdl/10915/151452
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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