RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
- Autores
- Castellani, Lucas Gabriel; Luchetti, Abril; Nilsson, Juliet Fernanda; Pérez Giménez, Julieta; Struck, Ben; Schlüter, Andreas; Pühler, Alfred; Niehaus, Karsten; Romero, David; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.
Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados".
Instituto de Biotecnología y Biología Molecular - Materia
-
Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/151452
Ver los metadatos del registro completo
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RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial PlasmidsCastellani, Lucas GabrielLuchetti, AbrilNilsson, Juliet FernandaPérez Giménez, JulietaStruck, BenSchlüter, AndreasPühler, AlfredNiehaus, KarstenRomero, DavidPistorio, MarianoTorres Tejerizo, Gonzalo ArturoCiencias ExactasRhizobiaplasmidconjugationRhizobiumgene regulationRhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados".Instituto de Biotecnología y Biología Molecular2022-09-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/151452enginfo:eu-repo/semantics/altIdentifier/issn/2150-7511info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.01949-22info:eu-repo/semantics/reference/hdl/10915/140513info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:39:01Zoai:sedici.unlp.edu.ar:10915/151452Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:39:01.822SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
title |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
spellingShingle |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids Castellani, Lucas Gabriel Ciencias Exactas Rhizobia plasmid conjugation Rhizobium gene regulation |
title_short |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
title_full |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
title_fullStr |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
title_full_unstemmed |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
title_sort |
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids |
dc.creator.none.fl_str_mv |
Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Struck, Ben Schlüter, Andreas Pühler, Alfred Niehaus, Karsten Romero, David Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo |
author |
Castellani, Lucas Gabriel |
author_facet |
Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Struck, Ben Schlüter, Andreas Pühler, Alfred Niehaus, Karsten Romero, David Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo |
author_role |
author |
author2 |
Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Struck, Ben Schlüter, Andreas Pühler, Alfred Niehaus, Karsten Romero, David Pistorio, Mariano Torres Tejerizo, Gonzalo Arturo |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Rhizobia plasmid conjugation Rhizobium gene regulation |
topic |
Ciencias Exactas Rhizobia plasmid conjugation Rhizobium gene regulation |
dc.description.none.fl_txt_mv |
Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids. Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados". Instituto de Biotecnología y Biología Molecular |
description |
Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/151452 |
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http://sedici.unlp.edu.ar/handle/10915/151452 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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