RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids

Autores
Castellani, Lucas Gabriel; Luchetti, Abril; Nilsson, Juliet Fernanda; Pérez Giménez, Julieta; Struck, Ben; Schlüter, Andreas; Pühler, Alfred; Niehaus, Karsten; Romero, David; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.
Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados".
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/151452

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network_name_str SEDICI (UNLP)
spelling RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial PlasmidsCastellani, Lucas GabrielLuchetti, AbrilNilsson, Juliet FernandaPérez Giménez, JulietaStruck, BenSchlüter, AndreasPühler, AlfredNiehaus, KarstenRomero, DavidPistorio, MarianoTorres Tejerizo, Gonzalo ArturoCiencias ExactasRhizobiaplasmidconjugationRhizobiumgene regulationRhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados".Instituto de Biotecnología y Biología Molecular2022-09-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/151452enginfo:eu-repo/semantics/altIdentifier/issn/2150-7511info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.01949-22info:eu-repo/semantics/reference/hdl/10915/140513info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:39:01Zoai:sedici.unlp.edu.ar:10915/151452Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:39:01.822SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
spellingShingle RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
Castellani, Lucas Gabriel
Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
title_short RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_full RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_fullStr RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_full_unstemmed RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
title_sort RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
dc.creator.none.fl_str_mv Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author Castellani, Lucas Gabriel
author_facet Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author_role author
author2 Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Struck, Ben
Schlüter, Andreas
Pühler, Alfred
Niehaus, Karsten
Romero, David
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
topic Ciencias Exactas
Rhizobia
plasmid
conjugation
Rhizobium
gene regulation
dc.description.none.fl_txt_mv Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.
Puede accederse a los datos con los que se realizó este trabajo haciendo clic en "Documentos relacionados".
Instituto de Biotecnología y Biología Molecular
description Rhizobia are Gram-negative bacteria that are able to establish a nitrogenfixing symbiotic interaction with leguminous plants. Rhizobia genomes usually harbor several plasmids which can be transferred to other organisms by conjugation. Two main mechanisms of the regulation of rhizobial plasmid transfer have been described: quorum sensing (QS) and the rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have recently been described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pRfaLPU83a from Rhizobium favelukesii LPU83, a plasmid that shows a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory system, we show evidence of the functions of these genes in different genomic backgrounds and confirm that homologous proteins from non-closely related organisms have the same functions. These findings set up the basis for a new regulatory circuit of the conjugative transfer of plasmids.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/151452
url http://sedici.unlp.edu.ar/handle/10915/151452
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2150-7511
info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.01949-22
info:eu-repo/semantics/reference/hdl/10915/140513
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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