Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
- Autores
- Rodríguez, María; Oteiza, Juan Martín; Giannuzzi, Leda; Zaritzky, Noemí Elisabet
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos - Materia
-
Química
Mutagenicity
UV radiation
Orange juice
Ames test
Ascorbic acid - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104004
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Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juiceRodríguez, MaríaOteiza, Juan MartínGiannuzzi, LedaZaritzky, Noemí ElisabetQuímicaMutagenicityUV radiationOrange juiceAmes testAscorbic acidUV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf315-330http://sedici.unlp.edu.ar/handle/10915/104004enginfo:eu-repo/semantics/altIdentifier/issn/1029-0486info:eu-repo/semantics/altIdentifier/doi/10.1080/02772248.2016.1178752info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:38Zoai:sedici.unlp.edu.ar:10915/104004Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:39.092SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
title |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
spellingShingle |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice Rodríguez, María Química Mutagenicity UV radiation Orange juice Ames test Ascorbic acid |
title_short |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
title_full |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
title_fullStr |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
title_full_unstemmed |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
title_sort |
Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice |
dc.creator.none.fl_str_mv |
Rodríguez, María Oteiza, Juan Martín Giannuzzi, Leda Zaritzky, Noemí Elisabet |
author |
Rodríguez, María |
author_facet |
Rodríguez, María Oteiza, Juan Martín Giannuzzi, Leda Zaritzky, Noemí Elisabet |
author_role |
author |
author2 |
Oteiza, Juan Martín Giannuzzi, Leda Zaritzky, Noemí Elisabet |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Química Mutagenicity UV radiation Orange juice Ames test Ascorbic acid |
topic |
Química Mutagenicity UV radiation Orange juice Ames test Ascorbic acid |
dc.description.none.fl_txt_mv |
UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation. Centro de Investigación y Desarrollo en Criotecnología de Alimentos |
description |
UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/104004 |
url |
http://sedici.unlp.edu.ar/handle/10915/104004 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1029-0486 info:eu-repo/semantics/altIdentifier/doi/10.1080/02772248.2016.1178752 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf 315-330 |
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reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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