Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice

Autores
Rodríguez, María; Oteiza, Juan Martín; Giannuzzi, Leda; Zaritzky, Noemí Elisabet
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Química
Mutagenicity
UV radiation
Orange juice
Ames test
Ascorbic acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104004

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network_name_str SEDICI (UNLP)
spelling Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juiceRodríguez, MaríaOteiza, Juan MartínGiannuzzi, LedaZaritzky, Noemí ElisabetQuímicaMutagenicityUV radiationOrange juiceAmes testAscorbic acidUV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf315-330http://sedici.unlp.edu.ar/handle/10915/104004enginfo:eu-repo/semantics/altIdentifier/issn/1029-0486info:eu-repo/semantics/altIdentifier/doi/10.1080/02772248.2016.1178752info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:22:38Zoai:sedici.unlp.edu.ar:10915/104004Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:22:39.092SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
title Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
spellingShingle Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
Rodríguez, María
Química
Mutagenicity
UV radiation
Orange juice
Ames test
Ascorbic acid
title_short Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
title_full Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
title_fullStr Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
title_full_unstemmed Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
title_sort Evaluation of mutagenicity associated with <i>Escherichia coli</i> inactivation in UV-treated orange juice
dc.creator.none.fl_str_mv Rodríguez, María
Oteiza, Juan Martín
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
author Rodríguez, María
author_facet Rodríguez, María
Oteiza, Juan Martín
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
author_role author
author2 Oteiza, Juan Martín
Giannuzzi, Leda
Zaritzky, Noemí Elisabet
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Mutagenicity
UV radiation
Orange juice
Ames test
Ascorbic acid
topic Química
Mutagenicity
UV radiation
Orange juice
Ames test
Ascorbic acid
dc.description.none.fl_txt_mv UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description UV radiation is an alternative technology for the elimination of pathogenic micro-organisms in liquid food. The objectives of this work were to investigate mutagenesis using the Ames test in : (1) fresh squeezed orange juice submitted to UV radiation doses required to achieve 5 and 10 log10 reductions of different strains of Escherichia coli serotype O157:H7 at two temperatures, and (2) commercial pasteurized orange juice submitted to thermal treatment. Two histidine-auxotrophic strains, Salmonella Typhimurium TA98 and TA97a, were used in the Ames test with and without metabolic activation. In commercial pasteurized orange juices, mutagenesis was not observed with S. Typhimurium TA98 strain with and without metabolic activation, but high mutagenic effect was observed using TA97a with metabolic activation. UV radiation did not affect the stability of ascorbic acid in aqueous solution. However, degradation of ascorbic acid was observed when UV treatment was performed on squeezed orange juice, following first order kinetics. UV treatment required to achieve 5 log10 reductions (1.5 J/cm2) did not show a mutagenic effect with and without metabolic activation using the tested strains, but a higher UV radiation dose necessary to produce 10 log10 reductions (3.0 J/cm2) showed mutagenicity with metabolic activation.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104004
url http://sedici.unlp.edu.ar/handle/10915/104004
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1029-0486
info:eu-repo/semantics/altIdentifier/doi/10.1080/02772248.2016.1178752
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
315-330
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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