Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution

Autores
Pastor, Rocío; Puyssegur, Juliana; De la Guardia, M. Paula; Sarmiento Varón, Lindybeth; Beccaglia, Gladys; Spada, Nicolás; Paes de Lima, Andrea; Collado, M. Soledad; Blanco, Andrés; Aspe Scetti, Isabel; Arabolaza, M. Elena; Paoli, Bibiana; Chirdo, Fernando Gabriel; Arana, Eloisa Irene
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background The tonsils operate as a protection ring of mucosa at the gates of the upper aero-digestive tract. They show similarities with lymph nodes and participate as inductive organs of systemic and mucosal immunity. Based on the reduction of their size since puberty, they are thought to experience involution in adulthood. In this context, we have used tonsillar mononuclear cells (TMC) isolated from patients at different stages of life, to study the effect of ageing and the concomitant persistent inflammation on these immune cells. Results We found an age-dependent reduction in the proportion of germinal center B cell population (BGC) and its T cell counterpart (T follicular helper germinal center cells, TfhGC). Also, we demonstrated an increment in the percentage of local memory B cells and mantle zone T follicular helper cells (mTfh). Furthermore, younger tonsils rendered higher proportion of proliferative immune cells within the freshly isolated TMC fraction than those from older ones. We demonstrated the accumulation of a B cell subset (CD20⁺CD39ʰⁱᵍʰCD73⁺ cells) metabolically adapted to catabolize adenosine triphosphate (ATP) as patients get older. To finish, tonsillar B cells from patients at different ages did not show differences in their proliferative response to stimulation ex vivo, in bulk TMC cultures. Conclusions This paper sheds light on the changing aspects of the immune cellular landscape, over the course of time and constant exposure, at the entrance of the respiratory and digestive systems. Our findings support the notion that there is a re-modelling of the immune functionality of the excised tonsils over time. They are indicative of a transition from an effector type of immune response, typically oriented to reduce pathogen burden early in life, to the development of an immunosuppressive microenvironment at later stages, when tissue damage control gets critical provided the time passed under immune attack. Noteworthy, when isolated from such histologic microenvironment, older tonsillar B cells seem to level their proliferation capacity with the younger ones. Understanding these features will not only contribute to comprehend the differences in susceptibility to pathogens among children and adults but would also impact on vaccine developments intended to target these relevant mucosal sites.
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Biología
Tonsils
Mucosa
Immunity
Ageing
Inflammation
Regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/167633

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network_name_str SEDICI (UNLP)
spelling Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involutionPastor, RocíoPuyssegur, JulianaDe la Guardia, M. PaulaSarmiento Varón, LindybethBeccaglia, GladysSpada, NicolásPaes de Lima, AndreaCollado, M. SoledadBlanco, AndrésAspe Scetti, IsabelArabolaza, M. ElenaPaoli, BibianaChirdo, Fernando GabrielArana, Eloisa IreneBiologíaTonsilsMucosaImmunityAgeingInflammationRegulationBackground The tonsils operate as a protection ring of mucosa at the gates of the upper aero-digestive tract. They show similarities with lymph nodes and participate as inductive organs of systemic and mucosal immunity. Based on the reduction of their size since puberty, they are thought to experience involution in adulthood. In this context, we have used tonsillar mononuclear cells (TMC) isolated from patients at different stages of life, to study the effect of ageing and the concomitant persistent inflammation on these immune cells. Results We found an age-dependent reduction in the proportion of germinal center B cell population (BGC) and its T cell counterpart (T follicular helper germinal center cells, TfhGC). Also, we demonstrated an increment in the percentage of local memory B cells and mantle zone T follicular helper cells (mTfh). Furthermore, younger tonsils rendered higher proportion of proliferative immune cells within the freshly isolated TMC fraction than those from older ones. We demonstrated the accumulation of a B cell subset (CD20⁺CD39ʰⁱᵍʰCD73⁺ cells) metabolically adapted to catabolize adenosine triphosphate (ATP) as patients get older. To finish, tonsillar B cells from patients at different ages did not show differences in their proliferative response to stimulation ex vivo, in bulk TMC cultures. Conclusions This paper sheds light on the changing aspects of the immune cellular landscape, over the course of time and constant exposure, at the entrance of the respiratory and digestive systems. Our findings support the notion that there is a re-modelling of the immune functionality of the excised tonsils over time. They are indicative of a transition from an effector type of immune response, typically oriented to reduce pathogen burden early in life, to the development of an immunosuppressive microenvironment at later stages, when tissue damage control gets critical provided the time passed under immune attack. Noteworthy, when isolated from such histologic microenvironment, older tonsillar B cells seem to level their proliferation capacity with the younger ones. Understanding these features will not only contribute to comprehend the differences in susceptibility to pathogens among children and adults but would also impact on vaccine developments intended to target these relevant mucosal sites.Instituto de Estudios Inmunológicos y Fisiopatológicos2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/167633enginfo:eu-repo/semantics/altIdentifier/issn/1742-4933info:eu-repo/semantics/altIdentifier/doi/10.1186/s12979-024-00425-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:44:40Zoai:sedici.unlp.edu.ar:10915/167633Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:44:40.796SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
title Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
spellingShingle Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
Pastor, Rocío
Biología
Tonsils
Mucosa
Immunity
Ageing
Inflammation
Regulation
title_short Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
title_full Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
title_fullStr Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
title_full_unstemmed Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
title_sort Role of germinal center and CD39ʰⁱᵍʰCD73⁺ B cells in the age-related tonsillar involution
dc.creator.none.fl_str_mv Pastor, Rocío
Puyssegur, Juliana
De la Guardia, M. Paula
Sarmiento Varón, Lindybeth
Beccaglia, Gladys
Spada, Nicolás
Paes de Lima, Andrea
Collado, M. Soledad
Blanco, Andrés
Aspe Scetti, Isabel
Arabolaza, M. Elena
Paoli, Bibiana
Chirdo, Fernando Gabriel
Arana, Eloisa Irene
author Pastor, Rocío
author_facet Pastor, Rocío
Puyssegur, Juliana
De la Guardia, M. Paula
Sarmiento Varón, Lindybeth
Beccaglia, Gladys
Spada, Nicolás
Paes de Lima, Andrea
Collado, M. Soledad
Blanco, Andrés
Aspe Scetti, Isabel
Arabolaza, M. Elena
Paoli, Bibiana
Chirdo, Fernando Gabriel
Arana, Eloisa Irene
author_role author
author2 Puyssegur, Juliana
De la Guardia, M. Paula
Sarmiento Varón, Lindybeth
Beccaglia, Gladys
Spada, Nicolás
Paes de Lima, Andrea
Collado, M. Soledad
Blanco, Andrés
Aspe Scetti, Isabel
Arabolaza, M. Elena
Paoli, Bibiana
Chirdo, Fernando Gabriel
Arana, Eloisa Irene
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Tonsils
Mucosa
Immunity
Ageing
Inflammation
Regulation
topic Biología
Tonsils
Mucosa
Immunity
Ageing
Inflammation
Regulation
dc.description.none.fl_txt_mv Background The tonsils operate as a protection ring of mucosa at the gates of the upper aero-digestive tract. They show similarities with lymph nodes and participate as inductive organs of systemic and mucosal immunity. Based on the reduction of their size since puberty, they are thought to experience involution in adulthood. In this context, we have used tonsillar mononuclear cells (TMC) isolated from patients at different stages of life, to study the effect of ageing and the concomitant persistent inflammation on these immune cells. Results We found an age-dependent reduction in the proportion of germinal center B cell population (BGC) and its T cell counterpart (T follicular helper germinal center cells, TfhGC). Also, we demonstrated an increment in the percentage of local memory B cells and mantle zone T follicular helper cells (mTfh). Furthermore, younger tonsils rendered higher proportion of proliferative immune cells within the freshly isolated TMC fraction than those from older ones. We demonstrated the accumulation of a B cell subset (CD20⁺CD39ʰⁱᵍʰCD73⁺ cells) metabolically adapted to catabolize adenosine triphosphate (ATP) as patients get older. To finish, tonsillar B cells from patients at different ages did not show differences in their proliferative response to stimulation ex vivo, in bulk TMC cultures. Conclusions This paper sheds light on the changing aspects of the immune cellular landscape, over the course of time and constant exposure, at the entrance of the respiratory and digestive systems. Our findings support the notion that there is a re-modelling of the immune functionality of the excised tonsils over time. They are indicative of a transition from an effector type of immune response, typically oriented to reduce pathogen burden early in life, to the development of an immunosuppressive microenvironment at later stages, when tissue damage control gets critical provided the time passed under immune attack. Noteworthy, when isolated from such histologic microenvironment, older tonsillar B cells seem to level their proliferation capacity with the younger ones. Understanding these features will not only contribute to comprehend the differences in susceptibility to pathogens among children and adults but would also impact on vaccine developments intended to target these relevant mucosal sites.
Instituto de Estudios Inmunológicos y Fisiopatológicos
description Background The tonsils operate as a protection ring of mucosa at the gates of the upper aero-digestive tract. They show similarities with lymph nodes and participate as inductive organs of systemic and mucosal immunity. Based on the reduction of their size since puberty, they are thought to experience involution in adulthood. In this context, we have used tonsillar mononuclear cells (TMC) isolated from patients at different stages of life, to study the effect of ageing and the concomitant persistent inflammation on these immune cells. Results We found an age-dependent reduction in the proportion of germinal center B cell population (BGC) and its T cell counterpart (T follicular helper germinal center cells, TfhGC). Also, we demonstrated an increment in the percentage of local memory B cells and mantle zone T follicular helper cells (mTfh). Furthermore, younger tonsils rendered higher proportion of proliferative immune cells within the freshly isolated TMC fraction than those from older ones. We demonstrated the accumulation of a B cell subset (CD20⁺CD39ʰⁱᵍʰCD73⁺ cells) metabolically adapted to catabolize adenosine triphosphate (ATP) as patients get older. To finish, tonsillar B cells from patients at different ages did not show differences in their proliferative response to stimulation ex vivo, in bulk TMC cultures. Conclusions This paper sheds light on the changing aspects of the immune cellular landscape, over the course of time and constant exposure, at the entrance of the respiratory and digestive systems. Our findings support the notion that there is a re-modelling of the immune functionality of the excised tonsils over time. They are indicative of a transition from an effector type of immune response, typically oriented to reduce pathogen burden early in life, to the development of an immunosuppressive microenvironment at later stages, when tissue damage control gets critical provided the time passed under immune attack. Noteworthy, when isolated from such histologic microenvironment, older tonsillar B cells seem to level their proliferation capacity with the younger ones. Understanding these features will not only contribute to comprehend the differences in susceptibility to pathogens among children and adults but would also impact on vaccine developments intended to target these relevant mucosal sites.
publishDate 2024
dc.date.none.fl_str_mv 2024
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info:eu-repo/semantics/publishedVersion
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language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s12979-024-00425-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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