First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)

Autores
Sánchez, Julieta; Alcalde, Leandro; Bolzán, Alejandro Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chelonoidis chilensis is an endangered tortoise that inhabits arid regions in Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the Argentinan distribution range together with samples from specimens of known morphotype but unknown provenance. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement was 2n=52 for all twenty-five tortoises analysed. Two different karyomorphs, termed A and B, were identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may originate from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analysed using Fluorescence In Situ Hybridisation (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telocentric chromosomes, and that interstitial telomeric sequences have not played a major role during the recent chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of one instead several species for the C. chilensis complex. Our data further suggest a possible correlation between chromosomal variation and geographical distribution: karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterises tortoises living in the Monte of Steps and Plains Eco-region. Morphology appears to vary independently of cytomorph variation.
Instituto Multidisciplinario de Biología Celular
Instituto de Limnología "Dr. Raúl A. Ringuelet"
Materia
Zoología
Chelonoidis chilensis
Cryptodira
Fluorescence In Situ hybridisation (FISH)
Karyotypic evolution
Testudines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/118335
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/118335
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)Sánchez, JulietaAlcalde, LeandroBolzán, Alejandro DanielZoologíaChelonoidis chilensisCryptodiraFluorescence In Situ hybridisation (FISH)Karyotypic evolutionTestudinesChelonoidis chilensis is an endangered tortoise that inhabits arid regions in Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the Argentinan distribution range together with samples from specimens of known morphotype but unknown provenance. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement was 2n=52 for all twenty-five tortoises analysed. Two different karyomorphs, termed A and B, were identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may originate from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analysed using Fluorescence In Situ Hybridisation (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telocentric chromosomes, and that interstitial telomeric sequences have not played a major role during the recent chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of one instead several species for the C. chilensis complex. Our data further suggest a possible correlation between chromosomal variation and geographical distribution: karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterises tortoises living in the Monte of Steps and Plains Eco-region. Morphology appears to vary independently of cytomorph variation.Instituto Multidisciplinario de Biología CelularInstituto de Limnología "Dr. Raúl A. Ringuelet"2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf83-89http://sedici.unlp.edu.ar/handle/10915/118335enginfo:eu-repo/semantics/altIdentifier/url/https://www.thebhs.org/publications/the-herpetological-journal/volume-25-number-2-april-2015/775-03-first-evidence-of-chromosomal-variation-within-i-chelonoidis-chilensis-i-testudines-testudinidae?highlight=WyJmaXJzdCIsImV2aWRlbmNlIiwib2YiLCJjaHJvbW9zb21hbCIsInZhcmlhdGlvbiIsIndpdGhpbiIsImNoZWxvbm9pZGlzIiwiY2hpbGVuc2lzIiwidGVzdHVkaW5lcyIsInRlc3R1ZGluaWRhZSIsImZpcnN0IGV2aWRlbmNlIiwiZmlyc3QgZXZpZGVuY2Ugb2YiLCJldmlkZW5jZSBvZiIsImV2aWRlbmNlIG9mIGNocm9tb3NvbWFsIiwib2YgY2hyb21vc29tYWwiLCJvZiBjaHJvbW9zb21hbCB2YXJpYXRpb24iLCJjaHJvbW9zb21hbCB2YXJpYXRpb24iLCJjaHJvbW9zb21hbCB2YXJpYXRpb24gd2l0aGluIiwidmFyaWF0aW9uIHdpdGhpbiIsImNoZWxvbm9pZGlzIGNoaWxlbnNpcyIsInRlc3R1ZGluZXMgdGVzdHVkaW5pZGFlIl0=info:eu-repo/semantics/altIdentifier/issn/0268-0130info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:59:56Zoai:sedici.unlp.edu.ar:10915/118335Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:59:57.354SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
spellingShingle First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
Sánchez, Julieta
Zoología
Chelonoidis chilensis
Cryptodira
Fluorescence In Situ hybridisation (FISH)
Karyotypic evolution
Testudines
title_short First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_full First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_fullStr First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_full_unstemmed First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_sort First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
dc.creator.none.fl_str_mv Sánchez, Julieta
Alcalde, Leandro
Bolzán, Alejandro Daniel
author Sánchez, Julieta
author_facet Sánchez, Julieta
Alcalde, Leandro
Bolzán, Alejandro Daniel
author_role author
author2 Alcalde, Leandro
Bolzán, Alejandro Daniel
author2_role author
author
dc.subject.none.fl_str_mv Zoología
Chelonoidis chilensis
Cryptodira
Fluorescence In Situ hybridisation (FISH)
Karyotypic evolution
Testudines
topic Zoología
Chelonoidis chilensis
Cryptodira
Fluorescence In Situ hybridisation (FISH)
Karyotypic evolution
Testudines
dc.description.none.fl_txt_mv Chelonoidis chilensis is an endangered tortoise that inhabits arid regions in Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the Argentinan distribution range together with samples from specimens of known morphotype but unknown provenance. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement was 2n=52 for all twenty-five tortoises analysed. Two different karyomorphs, termed A and B, were identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may originate from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analysed using Fluorescence In Situ Hybridisation (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telocentric chromosomes, and that interstitial telomeric sequences have not played a major role during the recent chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of one instead several species for the C. chilensis complex. Our data further suggest a possible correlation between chromosomal variation and geographical distribution: karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterises tortoises living in the Monte of Steps and Plains Eco-region. Morphology appears to vary independently of cytomorph variation.
Instituto Multidisciplinario de Biología Celular
Instituto de Limnología "Dr. Raúl A. Ringuelet"
description Chelonoidis chilensis is an endangered tortoise that inhabits arid regions in Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the Argentinan distribution range together with samples from specimens of known morphotype but unknown provenance. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement was 2n=52 for all twenty-five tortoises analysed. Two different karyomorphs, termed A and B, were identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may originate from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analysed using Fluorescence In Situ Hybridisation (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telocentric chromosomes, and that interstitial telomeric sequences have not played a major role during the recent chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of one instead several species for the C. chilensis complex. Our data further suggest a possible correlation between chromosomal variation and geographical distribution: karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterises tortoises living in the Monte of Steps and Plains Eco-region. Morphology appears to vary independently of cytomorph variation.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/118335
url http://sedici.unlp.edu.ar/handle/10915/118335
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thebhs.org/publications/the-herpetological-journal/volume-25-number-2-april-2015/775-03-first-evidence-of-chromosomal-variation-within-i-chelonoidis-chilensis-i-testudines-testudinidae?highlight=WyJmaXJzdCIsImV2aWRlbmNlIiwib2YiLCJjaHJvbW9zb21hbCIsInZhcmlhdGlvbiIsIndpdGhpbiIsImNoZWxvbm9pZGlzIiwiY2hpbGVuc2lzIiwidGVzdHVkaW5lcyIsInRlc3R1ZGluaWRhZSIsImZpcnN0IGV2aWRlbmNlIiwiZmlyc3QgZXZpZGVuY2Ugb2YiLCJldmlkZW5jZSBvZiIsImV2aWRlbmNlIG9mIGNocm9tb3NvbWFsIiwib2YgY2hyb21vc29tYWwiLCJvZiBjaHJvbW9zb21hbCB2YXJpYXRpb24iLCJjaHJvbW9zb21hbCB2YXJpYXRpb24iLCJjaHJvbW9zb21hbCB2YXJpYXRpb24gd2l0aGluIiwidmFyaWF0aW9uIHdpdGhpbiIsImNoZWxvbm9pZGlzIGNoaWxlbnNpcyIsInRlc3R1ZGluZXMgdGVzdHVkaW5pZGFlIl0=
info:eu-repo/semantics/altIdentifier/issn/0268-0130
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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