First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)

Autores
Sánchez, Julieta; Alcalde, Leandro; Bolzan, Alejandro Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chelonoidis chilensis is an endangered tortoise species that inhabits arid regions from Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the whole range of the distribution of the species in Argentina togheteher with samples from specimens of known morphotype but unknown procedence. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement of all tortoises analyzed (n=25) was 2n = 52. Two different karyomorphs, termed A and B, were also identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may be originated from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analyzed using fluorescence in situ hybridization (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telo¬centric chro¬mosomes, and that interstitial telomeric sequences have not played a major role during chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of only one species for the Chelonoidis chilensis complex, instead of two or three ones, as previously suggested by other authors. Finally, present data may suggest a possible correlation between chromosomal variation and geographical distribution of the wild tortoises studied, since karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterizes tortoises living in the Monte of Steps and Plains Eco-region, independently of the morphotype the tortoises have.
Fil: Sánchez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Alcalde, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Bolzan, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Materia
Testudines
Cryptodira
Chelonoidis Chilensis
Karyotypic Evolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23425
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23425
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)Sánchez, JulietaAlcalde, LeandroBolzan, Alejandro DanielTestudinesCryptodiraChelonoidis ChilensisKaryotypic Evolutionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chelonoidis chilensis is an endangered tortoise species that inhabits arid regions from Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the whole range of the distribution of the species in Argentina togheteher with samples from specimens of known morphotype but unknown procedence. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement of all tortoises analyzed (n=25) was 2n = 52. Two different karyomorphs, termed A and B, were also identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may be originated from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analyzed using fluorescence in situ hybridization (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telo¬centric chro¬mosomes, and that interstitial telomeric sequences have not played a major role during chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of only one species for the Chelonoidis chilensis complex, instead of two or three ones, as previously suggested by other authors. Finally, present data may suggest a possible correlation between chromosomal variation and geographical distribution of the wild tortoises studied, since karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterizes tortoises living in the Monte of Steps and Plains Eco-region, independently of the morphotype the tortoises have.Fil: Sánchez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Alcalde, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Bolzan, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaBritish Herpetological Society2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23425Sánchez, Julieta; Alcalde, Leandro; Bolzan, Alejandro Daniel; First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae); British Herpetological Society; Herpetological Journal; 25; 2; 4-2015; 83-890268-0130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.thebhs.org/publications/the-herpetological-journal/volume-25-number-2-april-2015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:11Zoai:ri.conicet.gov.ar:11336/23425instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:11.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
spellingShingle First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
Sánchez, Julieta
Testudines
Cryptodira
Chelonoidis Chilensis
Karyotypic Evolution
title_short First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_full First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_fullStr First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_full_unstemmed First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
title_sort First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)
dc.creator.none.fl_str_mv Sánchez, Julieta
Alcalde, Leandro
Bolzan, Alejandro Daniel
author Sánchez, Julieta
author_facet Sánchez, Julieta
Alcalde, Leandro
Bolzan, Alejandro Daniel
author_role author
author2 Alcalde, Leandro
Bolzan, Alejandro Daniel
author2_role author
author
dc.subject.none.fl_str_mv Testudines
Cryptodira
Chelonoidis Chilensis
Karyotypic Evolution
topic Testudines
Cryptodira
Chelonoidis Chilensis
Karyotypic Evolution
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chelonoidis chilensis is an endangered tortoise species that inhabits arid regions from Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the whole range of the distribution of the species in Argentina togheteher with samples from specimens of known morphotype but unknown procedence. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement of all tortoises analyzed (n=25) was 2n = 52. Two different karyomorphs, termed A and B, were also identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may be originated from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analyzed using fluorescence in situ hybridization (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telo¬centric chro¬mosomes, and that interstitial telomeric sequences have not played a major role during chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of only one species for the Chelonoidis chilensis complex, instead of two or three ones, as previously suggested by other authors. Finally, present data may suggest a possible correlation between chromosomal variation and geographical distribution of the wild tortoises studied, since karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterizes tortoises living in the Monte of Steps and Plains Eco-region, independently of the morphotype the tortoises have.
Fil: Sánchez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Alcalde, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Bolzan, Alejandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
description Chelonoidis chilensis is an endangered tortoise species that inhabits arid regions from Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the whole range of the distribution of the species in Argentina togheteher with samples from specimens of known morphotype but unknown procedence. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement of all tortoises analyzed (n=25) was 2n = 52. Two different karyomorphs, termed A and B, were also identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may be originated from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analyzed using fluorescence in situ hybridization (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of C. chilensis does not have telo¬centric chro¬mosomes, and that interstitial telomeric sequences have not played a major role during chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of only one species for the Chelonoidis chilensis complex, instead of two or three ones, as previously suggested by other authors. Finally, present data may suggest a possible correlation between chromosomal variation and geographical distribution of the wild tortoises studied, since karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterizes tortoises living in the Monte of Steps and Plains Eco-region, independently of the morphotype the tortoises have.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23425
Sánchez, Julieta; Alcalde, Leandro; Bolzan, Alejandro Daniel; First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae); British Herpetological Society; Herpetological Journal; 25; 2; 4-2015; 83-89
0268-0130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23425
identifier_str_mv Sánchez, Julieta; Alcalde, Leandro; Bolzan, Alejandro Daniel; First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae); British Herpetological Society; Herpetological Journal; 25; 2; 4-2015; 83-89
0268-0130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thebhs.org/publications/the-herpetological-journal/volume-25-number-2-april-2015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv British Herpetological Society
publisher.none.fl_str_mv British Herpetological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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