The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells

Autores
Taylor, J.; Pereyra, Andrea Soledad; Zhang, T.; Messi, M. L.; Wang, Z. M.; Hereñú, Claudia Beatriz; Kuan, P. F.; Delbono, O.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Médicas
Química
Cav, voltage-gated calcium channel
ChIP, chromatin immunoprecipitation
DM, differentiation medium
EC, excitation- contraction
EMSA, electrophoretic mobility shift assay
GK, guanylate kinase
GM, growth medium
H&E, hematoxylin & eosin
LMB, leptomycin-B
MPC, muscle progenitor cell
NC, noncanonical
NLS, nuclear localization sequence
RAd, recombinant adenoviral
SH3, Src homology 3
TSS, transcription start site
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85405
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85405
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cellsTaylor, J.Pereyra, Andrea SoledadZhang, T.Messi, M. L.Wang, Z. M.Hereñú, Claudia BeatrizKuan, P. F.Delbono, O.Ciencias MédicasQuímicaCav, voltage-gated calcium channelChIP, chromatin immunoprecipitationDM, differentiation mediumEC, excitation- contractionEMSA, electrophoretic mobility shift assayGK, guanylate kinaseGM, growth mediumH&E, hematoxylin & eosinLMB, leptomycin-BMPC, muscle progenitor cellNC, noncanonicalNLS, nuclear localization sequenceRAd, recombinant adenoviralSH3, Src homology 3TSS, transcription start siteVoltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.Instituto de Investigaciones Bioquímicas de La Plata2014-06-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf829-846http://sedici.unlp.edu.ar/handle/10915/85405enginfo:eu-repo/semantics/altIdentifier/issn/0021-9525info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201403021info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:25Zoai:sedici.unlp.edu.ar:10915/85405Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:26.239SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
title The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
spellingShingle The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
Taylor, J.
Ciencias Médicas
Química
Cav, voltage-gated calcium channel
ChIP, chromatin immunoprecipitation
DM, differentiation medium
EC, excitation- contraction
EMSA, electrophoretic mobility shift assay
GK, guanylate kinase
GM, growth medium
H&E, hematoxylin & eosin
LMB, leptomycin-B
MPC, muscle progenitor cell
NC, noncanonical
NLS, nuclear localization sequence
RAd, recombinant adenoviral
SH3, Src homology 3
TSS, transcription start site
title_short The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
title_full The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
title_fullStr The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
title_full_unstemmed The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
title_sort The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
dc.creator.none.fl_str_mv Taylor, J.
Pereyra, Andrea Soledad
Zhang, T.
Messi, M. L.
Wang, Z. M.
Hereñú, Claudia Beatriz
Kuan, P. F.
Delbono, O.
author Taylor, J.
author_facet Taylor, J.
Pereyra, Andrea Soledad
Zhang, T.
Messi, M. L.
Wang, Z. M.
Hereñú, Claudia Beatriz
Kuan, P. F.
Delbono, O.
author_role author
author2 Pereyra, Andrea Soledad
Zhang, T.
Messi, M. L.
Wang, Z. M.
Hereñú, Claudia Beatriz
Kuan, P. F.
Delbono, O.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Química
Cav, voltage-gated calcium channel
ChIP, chromatin immunoprecipitation
DM, differentiation medium
EC, excitation- contraction
EMSA, electrophoretic mobility shift assay
GK, guanylate kinase
GM, growth medium
H&E, hematoxylin & eosin
LMB, leptomycin-B
MPC, muscle progenitor cell
NC, noncanonical
NLS, nuclear localization sequence
RAd, recombinant adenoviral
SH3, Src homology 3
TSS, transcription start site
topic Ciencias Médicas
Química
Cav, voltage-gated calcium channel
ChIP, chromatin immunoprecipitation
DM, differentiation medium
EC, excitation- contraction
EMSA, electrophoretic mobility shift assay
GK, guanylate kinase
GM, growth medium
H&E, hematoxylin & eosin
LMB, leptomycin-B
MPC, muscle progenitor cell
NC, noncanonical
NLS, nuclear localization sequence
RAd, recombinant adenoviral
SH3, Src homology 3
TSS, transcription start site
dc.description.none.fl_txt_mv Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.
Instituto de Investigaciones Bioquímicas de La Plata
description Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85405
url http://sedici.unlp.edu.ar/handle/10915/85405
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0021-9525
info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201403021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/3.0/
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
dc.format.none.fl_str_mv application/pdf
829-846
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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