<i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins

Autores
Malamud, Mariano; Carasi, Paula; Bronsoms, Sílvia; Trejo, Sebastián Alejandro; Serradell, María de los Ángeles
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The S-layer is a proteinaceous envelope constituted by subunits that self-assemble to form a two-dimensional lattice that covers the surface of different species of Bacteria and Archaea, and it could be involved in cell recognition of microbes among other several distinct functions. In this work, both proteomic and genomic approaches were used to gain knowledge about the sequences of the S-layer protein (SLPs) encoding genes expressed by six aggregative and sixteen non-aggregative strains of potentially probiotic Lactobacillus kefiri Peptide mass fingerprint (PMF) analysis confirmed the identity of SLPs extracted from L. kefiri, and based on the homology with phylogenetically related species, primers located outside and inside the SLP-genes were employed to amplify genomic DNA. The O-glycosylation site SASSAS was found in all L. kefiri SLPs. Ten strains were selected for sequencing of the complete genes. The total length of the mature proteins varies from 492 to 576 amino acids, and all SLPs have a calculated pI between 9.37 and 9.60. The N-terminal region is relatively conserved and shows a high percentage of positively charged amino acids. Major differences among strains are found in the C-terminal region. Different groups could be distinguished regarding the mature SLPs and the similarities observed in the PMF spectra. Interestingly, SLPs of the aggregative strains are 100% homologous, although these strains were isolated from different kefir grains. This knowledge provides relevant data for better understanding of the mechanisms involved in SLPs functionality and could contribute to the development of products of biotechnological interest from potentially probiotic bacteria.
Facultad de Ciencias Exactas
Instituto Multidisciplinario de Biología Celular
Materia
Biología
Lactobacillus kefiri
Surface properties
Glycoproteins
S-layer proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/133566

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network_name_str SEDICI (UNLP)
spelling <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteinsMalamud, MarianoCarasi, PaulaBronsoms, SílviaTrejo, Sebastián AlejandroSerradell, María de los ÁngelesBiologíaLactobacillus kefiriSurface propertiesGlycoproteinsS-layer proteinsThe S-layer is a proteinaceous envelope constituted by subunits that self-assemble to form a two-dimensional lattice that covers the surface of different species of <i>Bacteria</i> and <i>Archaea</i>, and it could be involved in cell recognition of microbes among other several distinct functions. In this work, both proteomic and genomic approaches were used to gain knowledge about the sequences of the S-layer protein (SLPs) encoding genes expressed by six aggregative and sixteen non-aggregative strains of potentially probiotic <i>Lactobacillus kefiri</i> Peptide mass fingerprint (PMF) analysis confirmed the identity of SLPs extracted from <i>L. kefiri</i>, and based on the homology with phylogenetically related species, primers located outside and inside the SLP-genes were employed to amplify genomic DNA. The O-glycosylation site SASSAS was found in all <i>L. kefiri</i> SLPs. Ten strains were selected for sequencing of the complete genes. The total length of the mature proteins varies from 492 to 576 amino acids, and all SLPs have a calculated pI between 9.37 and 9.60. The N-terminal region is relatively conserved and shows a high percentage of positively charged amino acids. Major differences among strains are found in the C-terminal region. Different groups could be distinguished regarding the mature SLPs and the similarities observed in the PMF spectra. Interestingly, SLPs of the aggregative strains are 100% homologous, although these strains were isolated from different kefir grains. This knowledge provides relevant data for better understanding of the mechanisms involved in SLPs functionality and could contribute to the development of products of biotechnological interest from potentially probiotic bacteria.Facultad de Ciencias ExactasInstituto Multidisciplinario de Biología Celular2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf515-530http://sedici.unlp.edu.ar/handle/10915/133566enginfo:eu-repo/semantics/altIdentifier/issn/1572-9699info:eu-repo/semantics/altIdentifier/issn/0003-6072info:eu-repo/semantics/altIdentifier/doi/10.1007/s10482-016-0820-4info:eu-repo/semantics/altIdentifier/pmid/28004217info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:53Zoai:sedici.unlp.edu.ar:10915/133566Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:53.292SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
title <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
spellingShingle <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
Malamud, Mariano
Biología
Lactobacillus kefiri
Surface properties
Glycoproteins
S-layer proteins
title_short <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
title_full <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
title_fullStr <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
title_full_unstemmed <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
title_sort <i>Lactobacillus kefiri</i> shows inter-strain variations in the amino acid sequence of the S-layer proteins
dc.creator.none.fl_str_mv Malamud, Mariano
Carasi, Paula
Bronsoms, Sílvia
Trejo, Sebastián Alejandro
Serradell, María de los Ángeles
author Malamud, Mariano
author_facet Malamud, Mariano
Carasi, Paula
Bronsoms, Sílvia
Trejo, Sebastián Alejandro
Serradell, María de los Ángeles
author_role author
author2 Carasi, Paula
Bronsoms, Sílvia
Trejo, Sebastián Alejandro
Serradell, María de los Ángeles
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
Lactobacillus kefiri
Surface properties
Glycoproteins
S-layer proteins
topic Biología
Lactobacillus kefiri
Surface properties
Glycoproteins
S-layer proteins
dc.description.none.fl_txt_mv The S-layer is a proteinaceous envelope constituted by subunits that self-assemble to form a two-dimensional lattice that covers the surface of different species of <i>Bacteria</i> and <i>Archaea</i>, and it could be involved in cell recognition of microbes among other several distinct functions. In this work, both proteomic and genomic approaches were used to gain knowledge about the sequences of the S-layer protein (SLPs) encoding genes expressed by six aggregative and sixteen non-aggregative strains of potentially probiotic <i>Lactobacillus kefiri</i> Peptide mass fingerprint (PMF) analysis confirmed the identity of SLPs extracted from <i>L. kefiri</i>, and based on the homology with phylogenetically related species, primers located outside and inside the SLP-genes were employed to amplify genomic DNA. The O-glycosylation site SASSAS was found in all <i>L. kefiri</i> SLPs. Ten strains were selected for sequencing of the complete genes. The total length of the mature proteins varies from 492 to 576 amino acids, and all SLPs have a calculated pI between 9.37 and 9.60. The N-terminal region is relatively conserved and shows a high percentage of positively charged amino acids. Major differences among strains are found in the C-terminal region. Different groups could be distinguished regarding the mature SLPs and the similarities observed in the PMF spectra. Interestingly, SLPs of the aggregative strains are 100% homologous, although these strains were isolated from different kefir grains. This knowledge provides relevant data for better understanding of the mechanisms involved in SLPs functionality and could contribute to the development of products of biotechnological interest from potentially probiotic bacteria.
Facultad de Ciencias Exactas
Instituto Multidisciplinario de Biología Celular
description The S-layer is a proteinaceous envelope constituted by subunits that self-assemble to form a two-dimensional lattice that covers the surface of different species of <i>Bacteria</i> and <i>Archaea</i>, and it could be involved in cell recognition of microbes among other several distinct functions. In this work, both proteomic and genomic approaches were used to gain knowledge about the sequences of the S-layer protein (SLPs) encoding genes expressed by six aggregative and sixteen non-aggregative strains of potentially probiotic <i>Lactobacillus kefiri</i> Peptide mass fingerprint (PMF) analysis confirmed the identity of SLPs extracted from <i>L. kefiri</i>, and based on the homology with phylogenetically related species, primers located outside and inside the SLP-genes were employed to amplify genomic DNA. The O-glycosylation site SASSAS was found in all <i>L. kefiri</i> SLPs. Ten strains were selected for sequencing of the complete genes. The total length of the mature proteins varies from 492 to 576 amino acids, and all SLPs have a calculated pI between 9.37 and 9.60. The N-terminal region is relatively conserved and shows a high percentage of positively charged amino acids. Major differences among strains are found in the C-terminal region. Different groups could be distinguished regarding the mature SLPs and the similarities observed in the PMF spectra. Interestingly, SLPs of the aggregative strains are 100% homologous, although these strains were isolated from different kefir grains. This knowledge provides relevant data for better understanding of the mechanisms involved in SLPs functionality and could contribute to the development of products of biotechnological interest from potentially probiotic bacteria.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/133566
url http://sedici.unlp.edu.ar/handle/10915/133566
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1572-9699
info:eu-repo/semantics/altIdentifier/issn/0003-6072
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10482-016-0820-4
info:eu-repo/semantics/altIdentifier/pmid/28004217
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
515-530
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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