Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri

Autores
Carasi, Paula; Díaz, Mariángeles; Racedo, Silvia María; de Antoni, Graciela; Urdaci, Maria C; Serradell, María de los Ángeles
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
Fil: Carasi, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Díaz, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Racedo, Silvia María. Universite de Bordeaux; Francia
Fil: de Antoni, Graciela. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Urdaci, Maria C. Universite de Bordeaux; Francia
Fil: Serradell, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Materia
lactobacillus kefiri
antimicrobial
probiotics
dairy product
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32507
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32507
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiriCarasi, PaulaDíaz, MariángelesRacedo, Silvia Maríade Antoni, GracielaUrdaci, Maria CSerradell, María de los Ángeleslactobacillus kefiriantimicrobialprobioticsdairy producthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.Fil: Carasi, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: Díaz, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: Racedo, Silvia María. Universite de Bordeaux; FranciaFil: de Antoni, Graciela. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: Urdaci, Maria C. Universite de Bordeaux; FranciaFil: Serradell, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaHindawi Publishing Corporation2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32507Urdaci, Maria C; de Antoni, Graciela; Serradell, María de los Ángeles; Racedo, Silvia María; Carasi, Paula; Díaz, Mariángeles; et al.; Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri; Hindawi Publishing Corporation; BioMed Research International; 2014; 5-2014; 1-72314-6133CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2014/208974info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2014/208974/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:43Zoai:ri.conicet.gov.ar:11336/32507instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:44.26CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
title Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
spellingShingle Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
Carasi, Paula
lactobacillus kefiri
antimicrobial
probiotics
dairy product
title_short Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
title_full Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
title_fullStr Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
title_full_unstemmed Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
title_sort Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri
dc.creator.none.fl_str_mv Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
de Antoni, Graciela
Urdaci, Maria C
Serradell, María de los Ángeles
author Carasi, Paula
author_facet Carasi, Paula
Díaz, Mariángeles
Racedo, Silvia María
de Antoni, Graciela
Urdaci, Maria C
Serradell, María de los Ángeles
author_role author
author2 Díaz, Mariángeles
Racedo, Silvia María
de Antoni, Graciela
Urdaci, Maria C
Serradell, María de los Ángeles
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv lactobacillus kefiri
antimicrobial
probiotics
dairy product
topic lactobacillus kefiri
antimicrobial
probiotics
dairy product
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
Fil: Carasi, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Díaz, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Racedo, Silvia María. Universite de Bordeaux; Francia
Fil: de Antoni, Graciela. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Urdaci, Maria C. Universite de Bordeaux; Francia
Fil: Serradell, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
description Lactobacilli are generally regarded as safe; however, certain strains have been associated with cases of infection. Our workgroup has already assessed many functional properties of Lactobacillus kefiri, but parameters regarding safety must be studied before calling them probiotics. In this work, safety aspects and antimicrobial activity of L. kefiri strains were studied. None of the L. kefiri strains tested caused - or -hemolysis. All the strains were susceptible to tetracycline, clindamycin, streptomycin, ampicillin, erythromycin, kanamycin, and gentamicin; meanwhile, two strains were resistant to chloramphenicol. On the other hand, all L. kefiri strains were able to inhibit both Gram(+) and Gram(−) pathogens. Regarding the in vitro results, L. kefiri CIDCA 8348 was selected to perform in vivo studies. Mice treated daily with an oral dose of 108 CFU during 21 days showed no signs of pain, lethargy, dehydration, or diarrhea, and the histological studies were consistent with those findings. Moreover, no differences in proinflammatory cytokines secretion were observed between treated and control mice. No translocation of microorganisms to blood, spleen, or liver was observed. Regarding these findings, L. kefiri CIDCA 8348 is a microorganism isolated from a dairy product with a great potential as probiotic for human or animal use.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32507
Urdaci, Maria C; de Antoni, Graciela; Serradell, María de los Ángeles; Racedo, Silvia María; Carasi, Paula; Díaz, Mariángeles; et al.; Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri; Hindawi Publishing Corporation; BioMed Research International; 2014; 5-2014; 1-7
2314-6133
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32507
identifier_str_mv Urdaci, Maria C; de Antoni, Graciela; Serradell, María de los Ángeles; Racedo, Silvia María; Carasi, Paula; Díaz, Mariángeles; et al.; Safety Characterization and Antimicrobial Properties of Kefir-Isolated Lactobacillus kefiri; Hindawi Publishing Corporation; BioMed Research International; 2014; 5-2014; 1-7
2314-6133
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/208974
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2014/208974/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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