Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades

Autores
Chiumiento, Ignacio Rafael; Ituarte, Santiago; Sun, Jin; Qiu, Jian Wen; Heras, Horacio; Dreon, Marcos Sebastián
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tridecameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Naturales y Museo
Materia
Ciencias Exactas
Ciencias Naturales
Pomacea canaliculata
Gastropod hemocyanins
Gene structure study
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107884
Acceder
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network_name_str SEDICI (UNLP)
spelling Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod cladesChiumiento, Ignacio RafaelItuarte, SantiagoSun, JinQiu, Jian WenHeras, HoracioDreon, Marcos SebastiánCiencias ExactasCiencias NaturalesPomacea canaliculataGastropod hemocyaninsGene structure studyStructural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, <i>Pomacea canaliculata</i> (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tridecameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Naturales y Museo2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/107884enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6992001&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/pmid/31999773info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0228325info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:23:52Zoai:sedici.unlp.edu.ar:10915/107884Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:23:52.806SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
spellingShingle Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
Chiumiento, Ignacio Rafael
Ciencias Exactas
Ciencias Naturales
Pomacea canaliculata
Gastropod hemocyanins
Gene structure study
title_short Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_full Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_fullStr Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_full_unstemmed Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
title_sort Hemocyanin of the caenogastropod <i>Pomacea canaliculata</i> exhibits evolutionary differences among gastropod clades
dc.creator.none.fl_str_mv Chiumiento, Ignacio Rafael
Ituarte, Santiago
Sun, Jin
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
author Chiumiento, Ignacio Rafael
author_facet Chiumiento, Ignacio Rafael
Ituarte, Santiago
Sun, Jin
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
author_role author
author2 Ituarte, Santiago
Sun, Jin
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Ciencias Naturales
Pomacea canaliculata
Gastropod hemocyanins
Gene structure study
topic Ciencias Exactas
Ciencias Naturales
Pomacea canaliculata
Gastropod hemocyanins
Gene structure study
dc.description.none.fl_txt_mv Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, <i>Pomacea canaliculata</i> (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tridecameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.
Instituto de Investigaciones Bioquímicas de La Plata
Facultad de Ciencias Naturales y Museo
description Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, <i>Pomacea canaliculata</i> (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tridecameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107884
url http://sedici.unlp.edu.ar/handle/10915/107884
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/pmid/31999773
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0228325
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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