Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
- Autores
- Ferrero, Mariana C.; Fossati, Carlos A.; Rumbo, Martín; Baldi, Pablo C.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.
Facultad de Ciencias Exactas - Materia
-
Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/84395
Ver los metadatos del registro completo
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Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretionFerrero, Mariana C.Fossati, Carlos A.Rumbo, MartínBaldi, Pablo C.BioquímicaBrucellaGut epithelial cellsInflammatory responseIntracellular replicationEnfermedades Inflamatorias del IntestinoIn spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.Facultad de Ciencias Exactas2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf45-57http://sedici.unlp.edu.ar/handle/10915/84395enginfo:eu-repo/semantics/altIdentifier/issn/0928-8244info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695x.2012.00985.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:01Zoai:sedici.unlp.edu.ar:10915/84395Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:01.507SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
title |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
spellingShingle |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion Ferrero, Mariana C. Bioquímica Brucella Gut epithelial cells Inflammatory response Intracellular replication Enfermedades Inflamatorias del Intestino |
title_short |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
title_full |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
title_fullStr |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
title_full_unstemmed |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
title_sort |
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion |
dc.creator.none.fl_str_mv |
Ferrero, Mariana C. Fossati, Carlos A. Rumbo, Martín Baldi, Pablo C. |
author |
Ferrero, Mariana C. |
author_facet |
Ferrero, Mariana C. Fossati, Carlos A. Rumbo, Martín Baldi, Pablo C. |
author_role |
author |
author2 |
Fossati, Carlos A. Rumbo, Martín Baldi, Pablo C. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Bioquímica Brucella Gut epithelial cells Inflammatory response Intracellular replication Enfermedades Inflamatorias del Intestino |
topic |
Bioquímica Brucella Gut epithelial cells Inflammatory response Intracellular replication Enfermedades Inflamatorias del Intestino |
dc.description.none.fl_txt_mv |
In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells. Facultad de Ciencias Exactas |
description |
In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/84395 |
url |
http://sedici.unlp.edu.ar/handle/10915/84395 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0928-8244 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695x.2012.00985.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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