Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion

Autores
Ferrero, Mariana C.; Fossati, Carlos A.; Rumbo, Martín; Baldi, Pablo C.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.
Facultad de Ciencias Exactas
Materia
Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/84395

id SEDICI_c7266ce757d569d88c6618da470a318a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/84395
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretionFerrero, Mariana C.Fossati, Carlos A.Rumbo, MartínBaldi, Pablo C.BioquímicaBrucellaGut epithelial cellsInflammatory responseIntracellular replicationEnfermedades Inflamatorias del IntestinoIn spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.Facultad de Ciencias Exactas2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf45-57http://sedici.unlp.edu.ar/handle/10915/84395enginfo:eu-repo/semantics/altIdentifier/issn/0928-8244info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695x.2012.00985.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:01Zoai:sedici.unlp.edu.ar:10915/84395Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:01.507SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
spellingShingle Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
Ferrero, Mariana C.
Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
title_short Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_full Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_fullStr Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_full_unstemmed Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_sort Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
dc.creator.none.fl_str_mv Ferrero, Mariana C.
Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
author Ferrero, Mariana C.
author_facet Ferrero, Mariana C.
Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
author_role author
author2 Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
author2_role author
author
author
dc.subject.none.fl_str_mv Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
topic Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
dc.description.none.fl_txt_mv In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.
Facultad de Ciencias Exactas
description In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/84395
url http://sedici.unlp.edu.ar/handle/10915/84395
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0928-8244
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695x.2012.00985.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
45-57
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616032709246976
score 13.070432