Genetic typing of equine arteritis virus isolates from Argentina

Autores
Echeverría, María Gabriela; Díaz, Silvina; Metz, Germán Ernesto; Serena, María Soledad; Panei, Carlos Javier; Nosetto, Edgardo Omar
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Equine arteritis virus
Argentina
Phylogenetic analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136410
Acceder
id SEDICI_c64230630b86a5a67ed925ce31ac7b27
oai_identifier_str oai:sedici.unlp.edu.ar:10915/136410
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic typing of equine arteritis virus isolates from ArgentinaEcheverría, María GabrielaDíaz, SilvinaMetz, Germán ErnestoSerena, María SoledadPanei, Carlos JavierNosetto, Edgardo OmarCiencias VeterinariasEquine arteritis virusArgentinaPhylogenetic analysisWe report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.Facultad de Ciencias Veterinarias2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf313-320http://sedici.unlp.edu.ar/handle/10915/136410enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-007-0081-4info:eu-repo/semantics/altIdentifier/pmid/17294142info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:15:10Zoai:sedici.unlp.edu.ar:10915/136410Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:15:10.523SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic typing of equine arteritis virus isolates from Argentina
title Genetic typing of equine arteritis virus isolates from Argentina
spellingShingle Genetic typing of equine arteritis virus isolates from Argentina
Echeverría, María Gabriela
Ciencias Veterinarias
Equine arteritis virus
Argentina
Phylogenetic analysis
title_short Genetic typing of equine arteritis virus isolates from Argentina
title_full Genetic typing of equine arteritis virus isolates from Argentina
title_fullStr Genetic typing of equine arteritis virus isolates from Argentina
title_full_unstemmed Genetic typing of equine arteritis virus isolates from Argentina
title_sort Genetic typing of equine arteritis virus isolates from Argentina
dc.creator.none.fl_str_mv Echeverría, María Gabriela
Díaz, Silvina
Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
author Echeverría, María Gabriela
author_facet Echeverría, María Gabriela
Díaz, Silvina
Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
author_role author
author2 Díaz, Silvina
Metz, Germán Ernesto
Serena, María Soledad
Panei, Carlos Javier
Nosetto, Edgardo Omar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Equine arteritis virus
Argentina
Phylogenetic analysis
topic Ciencias Veterinarias
Equine arteritis virus
Argentina
Phylogenetic analysis
dc.description.none.fl_txt_mv We report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.
Facultad de Ciencias Veterinarias
description We report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136410
url http://sedici.unlp.edu.ar/handle/10915/136410
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/issn/1572-994x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-007-0081-4
info:eu-repo/semantics/altIdentifier/pmid/17294142
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
313-320
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1843532794770227200
score 13.001348

Publicaciones similares