Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation

Autores
Nikoloff, Noelia; Carranza Martín, Ana Cristina; Fabra, Mariana Carolina; Furnus, Cecilia Cristina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 5024h) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.
Instituto de Genética Veterinaria
Materia
Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/139973
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/139973
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturationNikoloff, NoeliaCarranza Martín, Ana CristinaFabra, Mariana CarolinaFurnus, Cecilia CristinaVeterinariaFormamide pesticideCumulus-oocyte complexApoptosisMTTSingle-cell gel electrophoresisOocyte nuclear maturationThe aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 50<sub>24h</sub>) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.Instituto de Genética Veterinaria2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf29188-29199http://sedici.unlp.edu.ar/handle/10915/139973enginfo:eu-repo/semantics/altIdentifier/issn/1614-7499info:eu-repo/semantics/altIdentifier/issn/0944-1344info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-021-12670-xinfo:eu-repo/semantics/altIdentifier/pmid/33550522info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:23:59Zoai:sedici.unlp.edu.ar:10915/139973Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:23:59.279SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
spellingShingle Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
Nikoloff, Noelia
Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
title_short Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_full Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_fullStr Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_full_unstemmed Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
title_sort Amitraz induced cytotoxic effect on bovine cumulus cells and impaired oocyte maturation
dc.creator.none.fl_str_mv Nikoloff, Noelia
Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
author Nikoloff, Noelia
author_facet Nikoloff, Noelia
Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
author_role author
author2 Carranza Martín, Ana Cristina
Fabra, Mariana Carolina
Furnus, Cecilia Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
topic Veterinaria
Formamide pesticide
Cumulus-oocyte complex
Apoptosis
MTT
Single-cell gel electrophoresis
Oocyte nuclear maturation
dc.description.none.fl_txt_mv The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 50<sub>24h</sub>) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.
Instituto de Genética Veterinaria
description The aim of this study was to evaluate the genotoxic and cytotoxic effects of amitraz (AMZ) on the primary culture of bovine cumulus cells (CC) and oocyte nuclear maturation. Cytotoxicity was evaluated by assessing mitochondrial activity with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Genotoxicity was estimated using the alkaline single cell gel electrophoresis (SCGE) assay. Apoptosis was detected with the Annexin V-affinity assay. The in vitro maturation test was performed in bovine oocytes. To understand AMZ action, glutathione content, superoxide dismutase enzyme activity, and lipid peroxidation were evaluated in CC. Results showed that AMZ lethal concentration (LC 50<sub>24h</sub>) for bovine CC was 32.55 μg/mL (MTT assay). A 25 μg/mL induced late apoptosis and necrotic cells (p < 0.05); however, DNA damage was decreased at the same concentration (SCGE assay; p < 0.05). A decrease in metaphase II was observed at 25 μg/mL, and degenerate oocytes were observed at 15 and 25 μg/mL (p < 0.05). None of the oxidative stress parameters evaluated showed significant differences. This study contributes to a better understanding of AMZ in this model, suggesting its potential cytotoxicity and impact on bovine reproduction.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/139973
url http://sedici.unlp.edu.ar/handle/10915/139973
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1614-7499
info:eu-repo/semantics/altIdentifier/issn/0944-1344
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-021-12670-x
info:eu-repo/semantics/altIdentifier/pmid/33550522
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
29188-29199
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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