Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte

Autores
Gutnisky, Cynthia; Morado, Sergio Adrián; Dalvit, Gabriel Carlos; Thompson, Jeremy G.; Cetica, Pablo Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to determine the effect of altering glycolytic pathway activity during bovine IVM on the meiotic maturation rate, oxidative activity, mitochondrial activity and the mitochondrial distribution within oocytes. Glycolytic activity was manipulated using two inhibitors (ATP, NaF) and a stimulator (AMP) of key enzymes of the pathway. Inhibition of glucose uptake, lactate production and meiotic maturation rates was observed when media were supplemented with ATP or NaF. The addition of AMP to the maturation medium had no effect on glucose uptake, lactate production or meiotic maturation. In the absence of gonadotrophin supplementation, AMP stimulated both glucose uptake and lactate production. However, AMP also decreased cytoplasmic maturation, as determined by early cleavage. During IVM, oocyte oxidative and mitochondrial activity was observed to increase at 15 and 22 h maturation. Inhibiting glycolysis with ATP or NaF led to a reduced oxidative and mitochondrial pattern compared with the respective control groups. Stimulation of the pathway with AMP increased oxidative and mitochondrial activity. A progressive mitochondrial migration to the central area was observed during maturation; oocytes treated with ATP, NaF or AMP showed limited migration. The present study reveals the effects of altering glycolytic pathway activity in cumulus?oocyte complexes, revealing the link between glycolysis of the cumulus-oocyte complex and the oxidative and mitochondrial activity of the oocyte.
Fil: Gutnisky, Cynthia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Thompson, Jeremy G.. University of Adelaide. School of Paediatrics and Reproductive Health. The Robinson Institute. Research Centre for Reproductive Health; Australia
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Materia
Cumulus-Oocyte Complex
Glycolysis
Maturation
Mitotracker Green
Redox Sensor Red
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3556
Acceder
id CONICETDig_dde07c2d83db3290a4a8080bf10bd4b4
oai_identifier_str oai:ri.conicet.gov.ar:11336/3556
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyteGutnisky, CynthiaMorado, Sergio AdriánDalvit, Gabriel CarlosThompson, Jeremy G.Cetica, Pablo DanielCumulus-Oocyte ComplexGlycolysisMaturationMitotracker GreenRedox Sensor Redhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to determine the effect of altering glycolytic pathway activity during bovine IVM on the meiotic maturation rate, oxidative activity, mitochondrial activity and the mitochondrial distribution within oocytes. Glycolytic activity was manipulated using two inhibitors (ATP, NaF) and a stimulator (AMP) of key enzymes of the pathway. Inhibition of glucose uptake, lactate production and meiotic maturation rates was observed when media were supplemented with ATP or NaF. The addition of AMP to the maturation medium had no effect on glucose uptake, lactate production or meiotic maturation. In the absence of gonadotrophin supplementation, AMP stimulated both glucose uptake and lactate production. However, AMP also decreased cytoplasmic maturation, as determined by early cleavage. During IVM, oocyte oxidative and mitochondrial activity was observed to increase at 15 and 22 h maturation. Inhibiting glycolysis with ATP or NaF led to a reduced oxidative and mitochondrial pattern compared with the respective control groups. Stimulation of the pathway with AMP increased oxidative and mitochondrial activity. A progressive mitochondrial migration to the central area was observed during maturation; oocytes treated with ATP, NaF or AMP showed limited migration. The present study reveals the effects of altering glycolytic pathway activity in cumulus?oocyte complexes, revealing the link between glycolysis of the cumulus-oocyte complex and the oxidative and mitochondrial activity of the oocyte.Fil: Gutnisky, Cynthia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Thompson, Jeremy G.. University of Adelaide. School of Paediatrics and Reproductive Health. The Robinson Institute. Research Centre for Reproductive Health; AustraliaFil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaCsiro Publishing2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3556Gutnisky, Cynthia; Morado, Sergio Adrián; Dalvit, Gabriel Carlos; Thompson, Jeremy G.; Cetica, Pablo Daniel; Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte; Csiro Publishing; Reproduction Fertility and Development; 25; 7; 8-2013; 1026-10351031-3613enginfo:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/paper/RD12193.htminfo:eu-repo/semantics/altIdentifier/doi/10.1071/RD12193info:eu-repo/semantics/altIdentifier/issn/1031-3613info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:41:29Zoai:ri.conicet.gov.ar:11336/3556instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:41:29.72CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
title Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
spellingShingle Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
Gutnisky, Cynthia
Cumulus-Oocyte Complex
Glycolysis
Maturation
Mitotracker Green
Redox Sensor Red
title_short Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
title_full Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
title_fullStr Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
title_full_unstemmed Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
title_sort Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte
dc.creator.none.fl_str_mv Gutnisky, Cynthia
Morado, Sergio Adrián
Dalvit, Gabriel Carlos
Thompson, Jeremy G.
Cetica, Pablo Daniel
author Gutnisky, Cynthia
author_facet Gutnisky, Cynthia
Morado, Sergio Adrián
Dalvit, Gabriel Carlos
Thompson, Jeremy G.
Cetica, Pablo Daniel
author_role author
author2 Morado, Sergio Adrián
Dalvit, Gabriel Carlos
Thompson, Jeremy G.
Cetica, Pablo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cumulus-Oocyte Complex
Glycolysis
Maturation
Mitotracker Green
Redox Sensor Red
topic Cumulus-Oocyte Complex
Glycolysis
Maturation
Mitotracker Green
Redox Sensor Red
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present study was to determine the effect of altering glycolytic pathway activity during bovine IVM on the meiotic maturation rate, oxidative activity, mitochondrial activity and the mitochondrial distribution within oocytes. Glycolytic activity was manipulated using two inhibitors (ATP, NaF) and a stimulator (AMP) of key enzymes of the pathway. Inhibition of glucose uptake, lactate production and meiotic maturation rates was observed when media were supplemented with ATP or NaF. The addition of AMP to the maturation medium had no effect on glucose uptake, lactate production or meiotic maturation. In the absence of gonadotrophin supplementation, AMP stimulated both glucose uptake and lactate production. However, AMP also decreased cytoplasmic maturation, as determined by early cleavage. During IVM, oocyte oxidative and mitochondrial activity was observed to increase at 15 and 22 h maturation. Inhibiting glycolysis with ATP or NaF led to a reduced oxidative and mitochondrial pattern compared with the respective control groups. Stimulation of the pathway with AMP increased oxidative and mitochondrial activity. A progressive mitochondrial migration to the central area was observed during maturation; oocytes treated with ATP, NaF or AMP showed limited migration. The present study reveals the effects of altering glycolytic pathway activity in cumulus?oocyte complexes, revealing the link between glycolysis of the cumulus-oocyte complex and the oxidative and mitochondrial activity of the oocyte.
Fil: Gutnisky, Cynthia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Thompson, Jeremy G.. University of Adelaide. School of Paediatrics and Reproductive Health. The Robinson Institute. Research Centre for Reproductive Health; Australia
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
description The aim of the present study was to determine the effect of altering glycolytic pathway activity during bovine IVM on the meiotic maturation rate, oxidative activity, mitochondrial activity and the mitochondrial distribution within oocytes. Glycolytic activity was manipulated using two inhibitors (ATP, NaF) and a stimulator (AMP) of key enzymes of the pathway. Inhibition of glucose uptake, lactate production and meiotic maturation rates was observed when media were supplemented with ATP or NaF. The addition of AMP to the maturation medium had no effect on glucose uptake, lactate production or meiotic maturation. In the absence of gonadotrophin supplementation, AMP stimulated both glucose uptake and lactate production. However, AMP also decreased cytoplasmic maturation, as determined by early cleavage. During IVM, oocyte oxidative and mitochondrial activity was observed to increase at 15 and 22 h maturation. Inhibiting glycolysis with ATP or NaF led to a reduced oxidative and mitochondrial pattern compared with the respective control groups. Stimulation of the pathway with AMP increased oxidative and mitochondrial activity. A progressive mitochondrial migration to the central area was observed during maturation; oocytes treated with ATP, NaF or AMP showed limited migration. The present study reveals the effects of altering glycolytic pathway activity in cumulus?oocyte complexes, revealing the link between glycolysis of the cumulus-oocyte complex and the oxidative and mitochondrial activity of the oocyte.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3556
Gutnisky, Cynthia; Morado, Sergio Adrián; Dalvit, Gabriel Carlos; Thompson, Jeremy G.; Cetica, Pablo Daniel; Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte; Csiro Publishing; Reproduction Fertility and Development; 25; 7; 8-2013; 1026-1035
1031-3613
url http://hdl.handle.net/11336/3556
identifier_str_mv Gutnisky, Cynthia; Morado, Sergio Adrián; Dalvit, Gabriel Carlos; Thompson, Jeremy G.; Cetica, Pablo Daniel; Glycolytic pathway activity: effect on in vitro maturation and oxidative metabolism of bovine oocyte; Csiro Publishing; Reproduction Fertility and Development; 25; 7; 8-2013; 1026-1035
1031-3613
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/paper/RD12193.htm
info:eu-repo/semantics/altIdentifier/doi/10.1071/RD12193
info:eu-repo/semantics/altIdentifier/issn/1031-3613
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083525650939904
score 13.22299

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