Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production

Autores
Fingermann, Matías; Fernández, Julieta; Sisti, Federico; Rodríguez, María Eugenia; Gatti, Blanca; Bottero, Daniela; Graieb, Augusto; Gaillard, María Emilia; González Ayala, Silvia Elena; Mooi, Frits R.; Lopardo, Horacio Ángel; Hozbor, Daniela Flavia
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, as in other countries, the number of pertussis cases has been increasing, even in highly vaccinated zones. Many reports suggest that the decline of vaccine efficacy due to antigenic shifts in the circulating Bordetella pertussis might be among the factors that contribute to pertussis re-emergence in different parts of the world. To evaluate the incidence of this factor in Argentina, we decided to characterize the circulating bacteria of an important demographic area of this country in comparison with the strain used for vaccine production. From 1997 to 2003 we collected nasopharyngeal samples from pediatric patients with signs of Bordetella infection hospitalized in the metropolitan area of Buenos Aires and La Plata, Argentina. From these samples we identified 28 B. pertussis, which were characterized by biochemical techniques, PCR, DNA fingerprint, prn and ptx genes sequencing, and lipopolysaccharides (LPS) pattern. BOX-PCR from B. pertussis isolates yielded one cluster containing 13 isolates and some smaller ones, being all fingerprints different from the vaccine strain. Differences between Argentinean circulating bacteria and the vaccine strain were also observed for the Prn and Ptx variants as well as for the LPS pattern. Moreover, this last pattern seemed to change over the years. In addition, we identified two B. bronchiseptica. The presence of this Bordetella species together with the observed differences between circulating B. pertussis and the strain used in vaccine production should be considered for the development of an improved vaccine.
Instituto de Biotecnología y Biología Molecular
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Bioquímica
Bordetella pertussis
Strain variation
Pertactin
Pertussis toxin
Vaccine strain
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/128729

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spelling Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine productionFingermann, MatíasFernández, JulietaSisti, FedericoRodríguez, María EugeniaGatti, BlancaBottero, DanielaGraieb, AugustoGaillard, María EmiliaGonzález Ayala, Silvia ElenaMooi, Frits R.Lopardo, Horacio ÁngelHozbor, Daniela FlaviaBioquímicaBordetella pertussisStrain variationPertactinPertussis toxinVaccine strainIn Argentina, as in other countries, the number of pertussis cases has been increasing, even in highly vaccinated zones. Many reports suggest that the decline of vaccine efficacy due to antigenic shifts in the circulating Bordetella pertussis might be among the factors that contribute to pertussis re-emergence in different parts of the world. To evaluate the incidence of this factor in Argentina, we decided to characterize the circulating bacteria of an important demographic area of this country in comparison with the strain used for vaccine production. From 1997 to 2003 we collected nasopharyngeal samples from pediatric patients with signs of Bordetella infection hospitalized in the metropolitan area of Buenos Aires and La Plata, Argentina. From these samples we identified 28 B. pertussis, which were characterized by biochemical techniques, PCR, DNA fingerprint, prn and ptx genes sequencing, and lipopolysaccharides (LPS) pattern. BOX-PCR from B. pertussis isolates yielded one cluster containing 13 isolates and some smaller ones, being all fingerprints different from the vaccine strain. Differences between Argentinean circulating bacteria and the vaccine strain were also observed for the Prn and Ptx variants as well as for the LPS pattern. Moreover, this last pattern seemed to change over the years. In addition, we identified two B. bronchiseptica. The presence of this Bordetella species together with the observed differences between circulating B. pertussis and the strain used in vaccine production should be considered for the development of an improved vaccine.Instituto de Biotecnología y Biología MolecularCentro de Investigación y Desarrollo en Fermentaciones Industriales2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf3513-3521http://sedici.unlp.edu.ar/handle/10915/128729enginfo:eu-repo/semantics/altIdentifier/issn/0264-410Xinfo:eu-repo/semantics/altIdentifier/issn/1358-8745info:eu-repo/semantics/altIdentifier/pmid/16545509info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2006.02.026info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:58Zoai:sedici.unlp.edu.ar:10915/128729Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:58.843SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
title Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
spellingShingle Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
Fingermann, Matías
Bioquímica
Bordetella pertussis
Strain variation
Pertactin
Pertussis toxin
Vaccine strain
title_short Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
title_full Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
title_fullStr Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
title_full_unstemmed Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
title_sort Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
dc.creator.none.fl_str_mv Fingermann, Matías
Fernández, Julieta
Sisti, Federico
Rodríguez, María Eugenia
Gatti, Blanca
Bottero, Daniela
Graieb, Augusto
Gaillard, María Emilia
González Ayala, Silvia Elena
Mooi, Frits R.
Lopardo, Horacio Ángel
Hozbor, Daniela Flavia
author Fingermann, Matías
author_facet Fingermann, Matías
Fernández, Julieta
Sisti, Federico
Rodríguez, María Eugenia
Gatti, Blanca
Bottero, Daniela
Graieb, Augusto
Gaillard, María Emilia
González Ayala, Silvia Elena
Mooi, Frits R.
Lopardo, Horacio Ángel
Hozbor, Daniela Flavia
author_role author
author2 Fernández, Julieta
Sisti, Federico
Rodríguez, María Eugenia
Gatti, Blanca
Bottero, Daniela
Graieb, Augusto
Gaillard, María Emilia
González Ayala, Silvia Elena
Mooi, Frits R.
Lopardo, Horacio Ángel
Hozbor, Daniela Flavia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Bordetella pertussis
Strain variation
Pertactin
Pertussis toxin
Vaccine strain
topic Bioquímica
Bordetella pertussis
Strain variation
Pertactin
Pertussis toxin
Vaccine strain
dc.description.none.fl_txt_mv In Argentina, as in other countries, the number of pertussis cases has been increasing, even in highly vaccinated zones. Many reports suggest that the decline of vaccine efficacy due to antigenic shifts in the circulating Bordetella pertussis might be among the factors that contribute to pertussis re-emergence in different parts of the world. To evaluate the incidence of this factor in Argentina, we decided to characterize the circulating bacteria of an important demographic area of this country in comparison with the strain used for vaccine production. From 1997 to 2003 we collected nasopharyngeal samples from pediatric patients with signs of Bordetella infection hospitalized in the metropolitan area of Buenos Aires and La Plata, Argentina. From these samples we identified 28 B. pertussis, which were characterized by biochemical techniques, PCR, DNA fingerprint, prn and ptx genes sequencing, and lipopolysaccharides (LPS) pattern. BOX-PCR from B. pertussis isolates yielded one cluster containing 13 isolates and some smaller ones, being all fingerprints different from the vaccine strain. Differences between Argentinean circulating bacteria and the vaccine strain were also observed for the Prn and Ptx variants as well as for the LPS pattern. Moreover, this last pattern seemed to change over the years. In addition, we identified two B. bronchiseptica. The presence of this Bordetella species together with the observed differences between circulating B. pertussis and the strain used in vaccine production should be considered for the development of an improved vaccine.
Instituto de Biotecnología y Biología Molecular
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description In Argentina, as in other countries, the number of pertussis cases has been increasing, even in highly vaccinated zones. Many reports suggest that the decline of vaccine efficacy due to antigenic shifts in the circulating Bordetella pertussis might be among the factors that contribute to pertussis re-emergence in different parts of the world. To evaluate the incidence of this factor in Argentina, we decided to characterize the circulating bacteria of an important demographic area of this country in comparison with the strain used for vaccine production. From 1997 to 2003 we collected nasopharyngeal samples from pediatric patients with signs of Bordetella infection hospitalized in the metropolitan area of Buenos Aires and La Plata, Argentina. From these samples we identified 28 B. pertussis, which were characterized by biochemical techniques, PCR, DNA fingerprint, prn and ptx genes sequencing, and lipopolysaccharides (LPS) pattern. BOX-PCR from B. pertussis isolates yielded one cluster containing 13 isolates and some smaller ones, being all fingerprints different from the vaccine strain. Differences between Argentinean circulating bacteria and the vaccine strain were also observed for the Prn and Ptx variants as well as for the LPS pattern. Moreover, this last pattern seemed to change over the years. In addition, we identified two B. bronchiseptica. The presence of this Bordetella species together with the observed differences between circulating B. pertussis and the strain used in vaccine production should be considered for the development of an improved vaccine.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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language eng
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info:eu-repo/semantics/altIdentifier/issn/1358-8745
info:eu-repo/semantics/altIdentifier/pmid/16545509
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2006.02.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
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