Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors

Autores
Peinetti, Ana Sol; Lake, Ryan J.; Cong, Wen; Cooper, Laura; Wu, Yuting; Ma, Yuan; Pawel, Gregory T.; Toimil-Molares, María Eugenia; Trautmann, Christina; Rong, Lijun; Mariñas, Benito J.; Azzaroni, Omar; Lu, Yi
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 10⁴ copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140266
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140266
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensorsPeinetti, Ana SolLake, Ryan J.Cong, WenCooper, LauraWu, YutingMa, YuanPawel, Gregory T.Toimil-Molares, María EugeniaTrautmann, ChristinaRong, LijunMariñas, Benito J.Azzaroni, OmarLu, YiMedicinaCiencias Exactasdirect detectionhuman adenovirusSARS-CoV-2Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 10⁴ copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/140266enginfo:eu-repo/semantics/altIdentifier/issn/2375-2548info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.abh2848info:eu-repo/semantics/altIdentifier/pmid/34550739info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:14Zoai:sedici.unlp.edu.ar:10915/140266Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:14.343SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
spellingShingle Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
Peinetti, Ana Sol
Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
title_short Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_fullStr Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full_unstemmed Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_sort Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
dc.creator.none.fl_str_mv Peinetti, Ana Sol
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
author Peinetti, Ana Sol
author_facet Peinetti, Ana Sol
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
author_role author
author2 Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil-Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito J.
Azzaroni, Omar
Lu, Yi
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
topic Medicina
Ciencias Exactas
direct detection
human adenovirus
SARS-CoV-2
dc.description.none.fl_txt_mv Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 10⁴ copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 10⁴ copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140266
url http://sedici.unlp.edu.ar/handle/10915/140266
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2375-2548
info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.abh2848
info:eu-repo/semantics/altIdentifier/pmid/34550739
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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