Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors

Autores
Peinetti, Ana S.; Lake, Ryan J.; Cong, Wen; Cooper, Laura; Wu, Yuting; Ma, Yuan; Pawel, Gregory T.; Toimil Molares, María Eugenia; Trautmann, Christina; Rong, Lijun; Mariñas, Benito; Azzaroni, Omar; Lu, Yi
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
Fil: Peinetti, Ana S.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Lake, Ryan J.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Cong, Wen. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Cooper, Laura. University of Illinois at Urbana; Estados Unidos
Fil: Wu, Yuting. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Ma, Yuan. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Pawel, Gregory T.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Toimil Molares, María Eugenia. No especifíca;
Fil: Trautmann, Christina. Universitat Technische Darmstadt; Alemania
Fil: Rong, Lijun. University of Illinois at Urbana; Estados Unidos
Fil: Mariñas, Benito. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Lu, Yi. University of Illinois. Urbana - Champaign; Estados Unidos
Materia
Nanopore sensors
Aptamers
SARS-CoV-2
Virus detection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211856
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensorsPeinetti, Ana S.Lake, Ryan J.Cong, WenCooper, LauraWu, YutingMa, YuanPawel, Gregory T.Toimil Molares, María EugeniaTrautmann, ChristinaRong, LijunMariñas, BenitoAzzaroni, OmarLu, YiNanopore sensorsAptamersSARS-CoV-2Virus detectionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.Fil: Peinetti, Ana S.. University of Illinois. Urbana - Champaign; Estados UnidosFil: Lake, Ryan J.. University of Illinois. Urbana - Champaign; Estados UnidosFil: Cong, Wen. University of Illinois. Urbana - Champaign; Estados UnidosFil: Cooper, Laura. University of Illinois at Urbana; Estados UnidosFil: Wu, Yuting. University of Illinois. Urbana - Champaign; Estados UnidosFil: Ma, Yuan. University of Illinois. Urbana - Champaign; Estados UnidosFil: Pawel, Gregory T.. University of Illinois. Urbana - Champaign; Estados UnidosFil: Toimil Molares, María Eugenia. No especifíca;Fil: Trautmann, Christina. Universitat Technische Darmstadt; AlemaniaFil: Rong, Lijun. University of Illinois at Urbana; Estados UnidosFil: Mariñas, Benito. University of Illinois. Urbana - Champaign; Estados UnidosFil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Lu, Yi. University of Illinois. Urbana - Champaign; Estados UnidosScience Advances is the American Association for the Advancement of Science2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211856Peinetti, Ana S.; Lake, Ryan J.; Cong, Wen; Cooper, Laura; Wu, Yuting; et al.; Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors; Science Advances is the American Association for the Advancement of Science; Science Advances; 7; 39; 9-2021; 1-132375-2548CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.abh2848info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:27Zoai:ri.conicet.gov.ar:11336/211856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:27.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
spellingShingle Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
Peinetti, Ana S.
Nanopore sensors
Aptamers
SARS-CoV-2
Virus detection
title_short Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_fullStr Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_full_unstemmed Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
title_sort Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
dc.creator.none.fl_str_mv Peinetti, Ana S.
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito
Azzaroni, Omar
Lu, Yi
author Peinetti, Ana S.
author_facet Peinetti, Ana S.
Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito
Azzaroni, Omar
Lu, Yi
author_role author
author2 Lake, Ryan J.
Cong, Wen
Cooper, Laura
Wu, Yuting
Ma, Yuan
Pawel, Gregory T.
Toimil Molares, María Eugenia
Trautmann, Christina
Rong, Lijun
Mariñas, Benito
Azzaroni, Omar
Lu, Yi
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanopore sensors
Aptamers
SARS-CoV-2
Virus detection
topic Nanopore sensors
Aptamers
SARS-CoV-2
Virus detection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
Fil: Peinetti, Ana S.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Lake, Ryan J.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Cong, Wen. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Cooper, Laura. University of Illinois at Urbana; Estados Unidos
Fil: Wu, Yuting. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Ma, Yuan. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Pawel, Gregory T.. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Toimil Molares, María Eugenia. No especifíca;
Fil: Trautmann, Christina. Universitat Technische Darmstadt; Alemania
Fil: Rong, Lijun. University of Illinois at Urbana; Estados Unidos
Fil: Mariñas, Benito. University of Illinois. Urbana - Champaign; Estados Unidos
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Lu, Yi. University of Illinois. Urbana - Champaign; Estados Unidos
description Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211856
Peinetti, Ana S.; Lake, Ryan J.; Cong, Wen; Cooper, Laura; Wu, Yuting; et al.; Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors; Science Advances is the American Association for the Advancement of Science; Science Advances; 7; 39; 9-2021; 1-13
2375-2548
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211856
identifier_str_mv Peinetti, Ana S.; Lake, Ryan J.; Cong, Wen; Cooper, Laura; Wu, Yuting; et al.; Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors; Science Advances is the American Association for the Advancement of Science; Science Advances; 7; 39; 9-2021; 1-13
2375-2548
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.abh2848
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Advances is the American Association for the Advancement of Science
publisher.none.fl_str_mv Science Advances is the American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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