Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats

Autores
Bonilla Bonilla, Adriana Milena; Bayley Agnoli, Matías; Ragone, María Inés; Consolini, Alicia Elvira
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Gomphrena perennis L. (Amaranthaceae) is a South American plant. In Argentine it is widely used as emollient, diuretic, hypotensive and for cardiac diseases. We previously demonstrated its antispasmodic effect on rat duodenum. This work evaluated the effects of Gomphrena tincture (GphT) on the contractile and energetic recovery of isolated rat hearts exposed to a model of stunning due to ischemia and reperfusion (I/R) as well as on the blood pressure of normotensive rats. GphT was prepared by aceration of leaves at 20% in ethanol 70°. Rats were anesthetized with 1.5g/kg urethane via i.p. and placed in a supine position with tracheal cannulation. Blood pressure (BP) was directly measured through an heparinized cannula in the internal carotid artery connected to a pressure transducer. To evaluate whether the GphT have a direct effect on hearts by an ischemic insult, isolated rat hearts were perfused at 37°C with control Krebs (C) in the chamber of a flow-calorimeter. After a 30-min equilibration period, the following protocols were done on each heart: perfusion with 0.1, 0.3 and 0.5% v/v GphT, followed by exposition to no-flow ischemia (I) during 20 min and to R with Krebs during 45 min. The contractility was evaluated from the left intraventricular pressure (P) which was measured simultaneously with the total heat rate (Ht) released by the heart. Basal BP of normotensive rats was 70.2 ± 15.2 mm Hg (n=4). GphT induced a dose-dependent hypotension (ΔBP: -13.6 ± 6.0 and -28.2 ± 11.6 mmHg respectively for 0.5% and 2% GphT, n=4). ΔBP was significantly reversed by the pre-treatment with L-NAME. Heart rate (HR) was not significantly modified by GphT at any dose, as neither after L-NAME. Perfusion of GphT in hearts produced a concentration-dependent positive inotropism. Nevertheless, 0.1% GphT significantly reduced cardiac contractility (P) and total muscle economy (Eco = P/Ht), as well as induced a diastolic contracture during R. The results suggest that: 1- The i.p. administration of GphT produced a dose dependent transitory hypotension and this effect was mediated by release of NO from the NO-synthase; 2- GphT induced a positive inotropism but increased the stunning of hearts exposed to I/R.
Facultad de Ciencias Exactas
Materia
Farmacia
Ciencias Exactas
Gomphrena perennis L
Blood pressure
Ischemic reperfusion
Calorimetry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/180430

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network_name_str SEDICI (UNLP)
spelling Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in ratsBonilla Bonilla, Adriana MilenaBayley Agnoli, MatíasRagone, María InésConsolini, Alicia ElviraFarmaciaCiencias ExactasGomphrena perennis LBlood pressureIschemic reperfusionCalorimetryGomphrena perennis L. (Amaranthaceae) is a South American plant. In Argentine it is widely used as emollient, diuretic, hypotensive and for cardiac diseases. We previously demonstrated its antispasmodic effect on rat duodenum. This work evaluated the effects of Gomphrena tincture (GphT) on the contractile and energetic recovery of isolated rat hearts exposed to a model of stunning due to ischemia and reperfusion (I/R) as well as on the blood pressure of normotensive rats. GphT was prepared by aceration of leaves at 20% in ethanol 70°. Rats were anesthetized with 1.5g/kg urethane via i.p. and placed in a supine position with tracheal cannulation. Blood pressure (BP) was directly measured through an heparinized cannula in the internal carotid artery connected to a pressure transducer. To evaluate whether the GphT have a direct effect on hearts by an ischemic insult, isolated rat hearts were perfused at 37°C with control Krebs (C) in the chamber of a flow-calorimeter. After a 30-min equilibration period, the following protocols were done on each heart: perfusion with 0.1, 0.3 and 0.5% v/v GphT, followed by exposition to no-flow ischemia (I) during 20 min and to R with Krebs during 45 min. The contractility was evaluated from the left intraventricular pressure (P) which was measured simultaneously with the total heat rate (Ht) released by the heart. Basal BP of normotensive rats was 70.2 ± 15.2 mm Hg (n=4). GphT induced a dose-dependent hypotension (ΔBP: -13.6 ± 6.0 and -28.2 ± 11.6 mmHg respectively for 0.5% and 2% GphT, n=4). ΔBP was significantly reversed by the pre-treatment with L-NAME. Heart rate (HR) was not significantly modified by GphT at any dose, as neither after L-NAME. Perfusion of GphT in hearts produced a concentration-dependent positive inotropism. Nevertheless, 0.1% GphT significantly reduced cardiac contractility (P) and total muscle economy (Eco = P/Ht), as well as induced a diastolic contracture during R. The results suggest that: 1- The i.p. administration of GphT produced a dose dependent transitory hypotension and this effect was mediated by release of NO from the NO-synthase; 2- GphT induced a positive inotropism but increased the stunning of hearts exposed to I/R.Facultad de Ciencias Exactas2017-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionResumenhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdf256-256http://sedici.unlp.edu.ar/handle/10915/180430enginfo:eu-repo/semantics/altIdentifier/isbn/978-958-59491-3-3info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:49:13Zoai:sedici.unlp.edu.ar:10915/180430Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:49:14.156SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
title Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
spellingShingle Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
Bonilla Bonilla, Adriana Milena
Farmacia
Ciencias Exactas
Gomphrena perennis L
Blood pressure
Ischemic reperfusion
Calorimetry
title_short Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
title_full Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
title_fullStr Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
title_full_unstemmed Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
title_sort Cardiovascular and cardioprotective effects of Gomphrena perennis L. (Amaranthaceae) dye tincture in rats
dc.creator.none.fl_str_mv Bonilla Bonilla, Adriana Milena
Bayley Agnoli, Matías
Ragone, María Inés
Consolini, Alicia Elvira
author Bonilla Bonilla, Adriana Milena
author_facet Bonilla Bonilla, Adriana Milena
Bayley Agnoli, Matías
Ragone, María Inés
Consolini, Alicia Elvira
author_role author
author2 Bayley Agnoli, Matías
Ragone, María Inés
Consolini, Alicia Elvira
author2_role author
author
author
dc.subject.none.fl_str_mv Farmacia
Ciencias Exactas
Gomphrena perennis L
Blood pressure
Ischemic reperfusion
Calorimetry
topic Farmacia
Ciencias Exactas
Gomphrena perennis L
Blood pressure
Ischemic reperfusion
Calorimetry
dc.description.none.fl_txt_mv Gomphrena perennis L. (Amaranthaceae) is a South American plant. In Argentine it is widely used as emollient, diuretic, hypotensive and for cardiac diseases. We previously demonstrated its antispasmodic effect on rat duodenum. This work evaluated the effects of Gomphrena tincture (GphT) on the contractile and energetic recovery of isolated rat hearts exposed to a model of stunning due to ischemia and reperfusion (I/R) as well as on the blood pressure of normotensive rats. GphT was prepared by aceration of leaves at 20% in ethanol 70°. Rats were anesthetized with 1.5g/kg urethane via i.p. and placed in a supine position with tracheal cannulation. Blood pressure (BP) was directly measured through an heparinized cannula in the internal carotid artery connected to a pressure transducer. To evaluate whether the GphT have a direct effect on hearts by an ischemic insult, isolated rat hearts were perfused at 37°C with control Krebs (C) in the chamber of a flow-calorimeter. After a 30-min equilibration period, the following protocols were done on each heart: perfusion with 0.1, 0.3 and 0.5% v/v GphT, followed by exposition to no-flow ischemia (I) during 20 min and to R with Krebs during 45 min. The contractility was evaluated from the left intraventricular pressure (P) which was measured simultaneously with the total heat rate (Ht) released by the heart. Basal BP of normotensive rats was 70.2 ± 15.2 mm Hg (n=4). GphT induced a dose-dependent hypotension (ΔBP: -13.6 ± 6.0 and -28.2 ± 11.6 mmHg respectively for 0.5% and 2% GphT, n=4). ΔBP was significantly reversed by the pre-treatment with L-NAME. Heart rate (HR) was not significantly modified by GphT at any dose, as neither after L-NAME. Perfusion of GphT in hearts produced a concentration-dependent positive inotropism. Nevertheless, 0.1% GphT significantly reduced cardiac contractility (P) and total muscle economy (Eco = P/Ht), as well as induced a diastolic contracture during R. The results suggest that: 1- The i.p. administration of GphT produced a dose dependent transitory hypotension and this effect was mediated by release of NO from the NO-synthase; 2- GphT induced a positive inotropism but increased the stunning of hearts exposed to I/R.
Facultad de Ciencias Exactas
description Gomphrena perennis L. (Amaranthaceae) is a South American plant. In Argentine it is widely used as emollient, diuretic, hypotensive and for cardiac diseases. We previously demonstrated its antispasmodic effect on rat duodenum. This work evaluated the effects of Gomphrena tincture (GphT) on the contractile and energetic recovery of isolated rat hearts exposed to a model of stunning due to ischemia and reperfusion (I/R) as well as on the blood pressure of normotensive rats. GphT was prepared by aceration of leaves at 20% in ethanol 70°. Rats were anesthetized with 1.5g/kg urethane via i.p. and placed in a supine position with tracheal cannulation. Blood pressure (BP) was directly measured through an heparinized cannula in the internal carotid artery connected to a pressure transducer. To evaluate whether the GphT have a direct effect on hearts by an ischemic insult, isolated rat hearts were perfused at 37°C with control Krebs (C) in the chamber of a flow-calorimeter. After a 30-min equilibration period, the following protocols were done on each heart: perfusion with 0.1, 0.3 and 0.5% v/v GphT, followed by exposition to no-flow ischemia (I) during 20 min and to R with Krebs during 45 min. The contractility was evaluated from the left intraventricular pressure (P) which was measured simultaneously with the total heat rate (Ht) released by the heart. Basal BP of normotensive rats was 70.2 ± 15.2 mm Hg (n=4). GphT induced a dose-dependent hypotension (ΔBP: -13.6 ± 6.0 and -28.2 ± 11.6 mmHg respectively for 0.5% and 2% GphT, n=4). ΔBP was significantly reversed by the pre-treatment with L-NAME. Heart rate (HR) was not significantly modified by GphT at any dose, as neither after L-NAME. Perfusion of GphT in hearts produced a concentration-dependent positive inotropism. Nevertheless, 0.1% GphT significantly reduced cardiac contractility (P) and total muscle economy (Eco = P/Ht), as well as induced a diastolic contracture during R. The results suggest that: 1- The i.p. administration of GphT produced a dose dependent transitory hypotension and this effect was mediated by release of NO from the NO-synthase; 2- GphT induced a positive inotropism but increased the stunning of hearts exposed to I/R.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
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