Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution

Autores
Percuoco, Cecilia Beatriz; Talavera Stéfani, Liliana Noelia; Rodríguez, Manuela Edith; González, Naiké Lucía; Crivello, Juan Fernando; Crisci, Jorge Víctor; Argüelles, Carina Francisca
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of Calophyllum brasiliense Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of C. brasiliense, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a C. brasiliense population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers petG-trnP, psbJ-petA and the trnL-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the trnL intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on trnL intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and Hd = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a C. brasiliense’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.
Facultad de Ciencias Naturales y Museo
Laboratorio de Sistemática y Biología Evolutiva
Materia
Ciencias Naturales
Calophyllum brasiliense
cpDNA
petG-trnP
psbJ-petA
trnL Intron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/101162

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network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distributionPercuoco, Cecilia BeatrizTalavera Stéfani, Liliana NoeliaRodríguez, Manuela EdithGonzález, Naiké LucíaCrivello, Juan FernandoCrisci, Jorge VíctorArgüelles, Carina FranciscaCiencias NaturalesCalophyllum brasiliensecpDNApetG-trnPpsbJ-petAtrnL IntronIn recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of <i>Calophyllum brasiliense</i> Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of <i>C. brasiliense</i>, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a <i>C. brasiliense</i> population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers <i>petG-trnP</i>, <i>psbJ-petA</i> and the <i>trnL</i>-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the <i>trnL</i> intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on <i>trnL</i> intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and <i>Hd</i> = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a <i>C. brasiliense</i>’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.Facultad de Ciencias Naturales y MuseoLaboratorio de Sistemática y Biología Evolutiva2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf310-319http://sedici.unlp.edu.ar/handle/10915/101162enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/38354info:eu-repo/semantics/altIdentifier/url/http://article.sciencepublishinggroup.com/html/10.11648.j.jps.20150306.14.htmlinfo:eu-repo/semantics/altIdentifier/issn/2331-0731info:eu-repo/semantics/altIdentifier/hdl/11336/38354info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:53:10Zoai:sedici.unlp.edu.ar:10915/101162Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:53:10.599SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
title Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
spellingShingle Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
Percuoco, Cecilia Beatriz
Ciencias Naturales
Calophyllum brasiliense
cpDNA
petG-trnP
psbJ-petA
trnL Intron
title_short Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
title_full Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
title_fullStr Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
title_full_unstemmed Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
title_sort Looking inside non-coding chloroplast regions of <i>Calophyllum brasiliense</i> Calophyllaceae) to understand its southernmost population distribution
dc.creator.none.fl_str_mv Percuoco, Cecilia Beatriz
Talavera Stéfani, Liliana Noelia
Rodríguez, Manuela Edith
González, Naiké Lucía
Crivello, Juan Fernando
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author Percuoco, Cecilia Beatriz
author_facet Percuoco, Cecilia Beatriz
Talavera Stéfani, Liliana Noelia
Rodríguez, Manuela Edith
González, Naiké Lucía
Crivello, Juan Fernando
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author_role author
author2 Talavera Stéfani, Liliana Noelia
Rodríguez, Manuela Edith
González, Naiké Lucía
Crivello, Juan Fernando
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Calophyllum brasiliense
cpDNA
petG-trnP
psbJ-petA
trnL Intron
topic Ciencias Naturales
Calophyllum brasiliense
cpDNA
petG-trnP
psbJ-petA
trnL Intron
dc.description.none.fl_txt_mv In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of <i>Calophyllum brasiliense</i> Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of <i>C. brasiliense</i>, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a <i>C. brasiliense</i> population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers <i>petG-trnP</i>, <i>psbJ-petA</i> and the <i>trnL</i>-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the <i>trnL</i> intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on <i>trnL</i> intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and <i>Hd</i> = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a <i>C. brasiliense</i>’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.
Facultad de Ciencias Naturales y Museo
Laboratorio de Sistemática y Biología Evolutiva
description In recent years the growing interest in the conservation of Paraná River’s riparian forest led to the discovery of botanical novelties for Argentina. Populations of <i>Calophyllum brasiliense</i> Camb. (Calophyllaceae), a typically flooded lowlands species, were identified in the remaining hygrophile forest of northeast Argentina and southeast Paraguay. Deforestation and flooding, due to the construction of dams, have caused these populations to suffer intensive fragmentation. The aim of this work was to infer phylogeographic relationships among five populations of <i>C. brasiliense</i>, three from Argentina and two from Paraguay, which represent the southernmost points of species’ distribution. We also compared them with samples of a <i>C. brasiliense</i> population from Mexico, the northernmost edge of the species distribution. The chloroplast intergenic spacers <i>petG-trnP</i>, <i>psbJ-petA</i> and the <i>trnL</i>-UAA chloroplast intron were amplified from leaves’ DNA. A total of 2234 bp were characterized once the three regions were analyzed. The three chloroplast regions showed nucleotide differences, represented by InDels, inversions and a few SNPs; however, only the <i>trnL</i> intron was selected for further phylogeographic analysis due to the amount of the information obtained for all populations. Based on <i>trnL</i> intron, it was possible to estimate nucleotide and haplotype diversity (π = 0.00237 and <i>Hd</i> = 0.29600, respectively). Three haplotypes were identified, which allowed Argentinean, Paraguayan and Mexican populations to be differentiated. Based on the three haplotypes found, we discuss and propose a model for a <i>C. brasiliense</i>’ geographic dispersion and historical colonization routes, including an alternative new one to the well-known of the Paraná River.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
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info:eu-repo/semantics/publishedVersion
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language eng
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info:eu-repo/semantics/altIdentifier/issn/2331-0731
info:eu-repo/semantics/altIdentifier/hdl/11336/38354
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
310-319
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