Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses

Autores
Percuoco, Cecilia Beatriz; Giménez, Lucas G.; Talavera Stefani, Liliana Noelia; Crisci, Jorge Víctor; Argüelles, Carina Francisca
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rpl32-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.
Laboratorio de Sistemática y Biología Evolutiva
Materia
Ingeniería Forestal
cpDNA
cpSSRs
Noncoding regions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/143751
Acceder
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network_name_str SEDICI (UNLP)
spelling Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analysesPercuoco, Cecilia BeatrizGiménez, Lucas G.Talavera Stefani, Liliana NoeliaCrisci, Jorge VíctorArgüelles, Carina FranciscaIngeniería ForestalcpDNAcpSSRsNoncoding regionsThe uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rpl32-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.Laboratorio de Sistemática y Biología Evolutiva2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf983-990http://sedici.unlp.edu.ar/handle/10915/143751enginfo:eu-repo/semantics/altIdentifier/issn/1007-662Xinfo:eu-repo/semantics/altIdentifier/issn/1993-0607info:eu-repo/semantics/altIdentifier/issn/1002-5618info:eu-repo/semantics/altIdentifier/doi/10.1007/s11676-017-0489-1info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:19Zoai:sedici.unlp.edu.ar:10915/143751Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:19.553SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
title Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
spellingShingle Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
Percuoco, Cecilia Beatriz
Ingeniería Forestal
cpDNA
cpSSRs
Noncoding regions
title_short Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
title_full Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
title_fullStr Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
title_full_unstemmed Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
title_sort Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses
dc.creator.none.fl_str_mv Percuoco, Cecilia Beatriz
Giménez, Lucas G.
Talavera Stefani, Liliana Noelia
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author Percuoco, Cecilia Beatriz
author_facet Percuoco, Cecilia Beatriz
Giménez, Lucas G.
Talavera Stefani, Liliana Noelia
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author_role author
author2 Giménez, Lucas G.
Talavera Stefani, Liliana Noelia
Crisci, Jorge Víctor
Argüelles, Carina Francisca
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ingeniería Forestal
cpDNA
cpSSRs
Noncoding regions
topic Ingeniería Forestal
cpDNA
cpSSRs
Noncoding regions
dc.description.none.fl_txt_mv The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rpl32-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.
Laboratorio de Sistemática y Biología Evolutiva
description The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rpl32-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/143751
url http://sedici.unlp.edu.ar/handle/10915/143751
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1007-662X
info:eu-repo/semantics/altIdentifier/issn/1993-0607
info:eu-repo/semantics/altIdentifier/issn/1002-5618
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11676-017-0489-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
983-990
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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