Bordetella pertussis modulates human macrophage defense gene expression

Autores
Valdez, Hugo Alberto; Oviedo, Juan Marcos; Gorgojo, Juan Pablo; Lamberti, Yanina Andrea; Rodríguez, María Eugenia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella pertussis, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that B. pertussis can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. In this work, we examined the interaction of B. pertussis with a human monocyte cell line (THP-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selected number of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phases of infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, while the number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of B. pertussis, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cell response. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections by those bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome of the interaction between B. pertussis and macrophages.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Adenylate cyclase
Bordetella pertussis
Host cell defense response
Intracellular survival
Pertussis toxin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85803
Acceder
id SEDICI_bfc505bee39daba3d314d214452dcaf0
oai_identifier_str oai:sedici.unlp.edu.ar:10915/85803
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Bordetella pertussis modulates human macrophage defense gene expressionValdez, Hugo AlbertoOviedo, Juan MarcosGorgojo, Juan PabloLamberti, Yanina AndreaRodríguez, María EugeniaCiencias ExactasAdenylate cyclaseBordetella pertussisHost cell defense responseIntracellular survivalPertussis toxin<i>Bordetella pertussis</i>, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that <i>B. pertussis</i> can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. In this work, we examined the interaction of <i>B. pertussis</i> with a human monocyte cell line (THP-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selected number of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phases of infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, while the number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of <i>B. pertussis</i>, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cell response. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections by those bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome of the interaction between <i>B. pertussis</i> and macrophages.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85803enginfo:eu-repo/semantics/altIdentifier/issn/2049-632Xinfo:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/ftw073info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:45Zoai:sedici.unlp.edu.ar:10915/85803Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:45.362SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Bordetella pertussis modulates human macrophage defense gene expression
title Bordetella pertussis modulates human macrophage defense gene expression
spellingShingle Bordetella pertussis modulates human macrophage defense gene expression
Valdez, Hugo Alberto
Ciencias Exactas
Adenylate cyclase
Bordetella pertussis
Host cell defense response
Intracellular survival
Pertussis toxin
title_short Bordetella pertussis modulates human macrophage defense gene expression
title_full Bordetella pertussis modulates human macrophage defense gene expression
title_fullStr Bordetella pertussis modulates human macrophage defense gene expression
title_full_unstemmed Bordetella pertussis modulates human macrophage defense gene expression
title_sort Bordetella pertussis modulates human macrophage defense gene expression
dc.creator.none.fl_str_mv Valdez, Hugo Alberto
Oviedo, Juan Marcos
Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Rodríguez, María Eugenia
author Valdez, Hugo Alberto
author_facet Valdez, Hugo Alberto
Oviedo, Juan Marcos
Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Rodríguez, María Eugenia
author_role author
author2 Oviedo, Juan Marcos
Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Rodríguez, María Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Adenylate cyclase
Bordetella pertussis
Host cell defense response
Intracellular survival
Pertussis toxin
topic Ciencias Exactas
Adenylate cyclase
Bordetella pertussis
Host cell defense response
Intracellular survival
Pertussis toxin
dc.description.none.fl_txt_mv <i>Bordetella pertussis</i>, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that <i>B. pertussis</i> can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. In this work, we examined the interaction of <i>B. pertussis</i> with a human monocyte cell line (THP-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selected number of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phases of infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, while the number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of <i>B. pertussis</i>, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cell response. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections by those bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome of the interaction between <i>B. pertussis</i> and macrophages.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description <i>Bordetella pertussis</i>, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that <i>B. pertussis</i> can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. In this work, we examined the interaction of <i>B. pertussis</i> with a human monocyte cell line (THP-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selected number of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phases of infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, while the number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of <i>B. pertussis</i>, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cell response. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections by those bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome of the interaction between <i>B. pertussis</i> and macrophages.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85803
url http://sedici.unlp.edu.ar/handle/10915/85803
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2049-632X
info:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/ftw073
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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