Transcriptional profiling of human macrophages during infection with Bordetella pertussis

Autores
Petráčková, Denisa; Farman, Mariam R.; Amman, Fabian; Linhartová, Irena; Dienstbier, Ana; Kumar, Dilip; Držmíšek, Jakub; Hofacker, Ivo; Rodriguez, Maria Eugenia; Večerek, Branislav
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
Fil: Petráčková, Denisa. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Farman, Mariam R.. University of Vienna. Institute for Theoretical Chemistry; Austria
Fil: Amman, Fabian. University of Vienna. Institute for Theoretical Chemistry; Austria. Medical University of Vienna. Division of Cell and Developmental Biology; Austria
Fil: Linhartová, Irena. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Molecular Biology of Bacterial Pathogens; República Checa
Fil: Dienstbier, Ana. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Kumar, Dilip. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Držmíšek, Jakub. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Hofacker, Ivo. University of Vienna. Institute for Theoretical Chemistry; Austria. University of Vienna. Faculty of Computer Science, Research Group Bioinformatics and Computational Biology; Austria
Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Večerek, Branislav. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Materia
BORDETELLA PERTUSSIS
HOST-PATHOGEN INTERACTION
INFECTION
INTRACELLULAR SURVIVAL
MACROPHAGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143520
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/143520
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptional profiling of human macrophages during infection with Bordetella pertussisPetráčková, DenisaFarman, Mariam R.Amman, FabianLinhartová, IrenaDienstbier, AnaKumar, DilipDržmíšek, JakubHofacker, IvoRodriguez, Maria EugeniaVečerek, BranislavBORDETELLA PERTUSSISHOST-PATHOGEN INTERACTIONINFECTIONINTRACELLULAR SURVIVALMACROPHAGEhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.Fil: Petráčková, Denisa. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República ChecaFil: Farman, Mariam R.. University of Vienna. Institute for Theoretical Chemistry; AustriaFil: Amman, Fabian. University of Vienna. Institute for Theoretical Chemistry; Austria. Medical University of Vienna. Division of Cell and Developmental Biology; AustriaFil: Linhartová, Irena. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Molecular Biology of Bacterial Pathogens; República ChecaFil: Dienstbier, Ana. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República ChecaFil: Kumar, Dilip. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República ChecaFil: Držmíšek, Jakub. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República ChecaFil: Hofacker, Ivo. University of Vienna. Institute for Theoretical Chemistry; Austria. University of Vienna. Faculty of Computer Science, Research Group Bioinformatics and Computational Biology; AustriaFil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Večerek, Branislav. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República ChecaLandes Bioscience2020-02-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143520Petráčková, Denisa; Farman, Mariam R.; Amman, Fabian; Linhartová, Irena; Dienstbier, Ana; et al.; Transcriptional profiling of human macrophages during infection with Bordetella pertussis; Landes Bioscience; Rna Biology; 17; 5; 19-2-2020; 731-7421547-62861555-8584CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/15476286.2020.1727694info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:33Zoai:ri.conicet.gov.ar:11336/143520instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:33.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional profiling of human macrophages during infection with Bordetella pertussis
title Transcriptional profiling of human macrophages during infection with Bordetella pertussis
spellingShingle Transcriptional profiling of human macrophages during infection with Bordetella pertussis
Petráčková, Denisa
BORDETELLA PERTUSSIS
HOST-PATHOGEN INTERACTION
INFECTION
INTRACELLULAR SURVIVAL
MACROPHAGE
title_short Transcriptional profiling of human macrophages during infection with Bordetella pertussis
title_full Transcriptional profiling of human macrophages during infection with Bordetella pertussis
title_fullStr Transcriptional profiling of human macrophages during infection with Bordetella pertussis
title_full_unstemmed Transcriptional profiling of human macrophages during infection with Bordetella pertussis
title_sort Transcriptional profiling of human macrophages during infection with Bordetella pertussis
dc.creator.none.fl_str_mv Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodriguez, Maria Eugenia
Večerek, Branislav
author Petráčková, Denisa
author_facet Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodriguez, Maria Eugenia
Večerek, Branislav
author_role author
author2 Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodriguez, Maria Eugenia
Večerek, Branislav
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BORDETELLA PERTUSSIS
HOST-PATHOGEN INTERACTION
INFECTION
INTRACELLULAR SURVIVAL
MACROPHAGE
topic BORDETELLA PERTUSSIS
HOST-PATHOGEN INTERACTION
INFECTION
INTRACELLULAR SURVIVAL
MACROPHAGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
Fil: Petráčková, Denisa. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Farman, Mariam R.. University of Vienna. Institute for Theoretical Chemistry; Austria
Fil: Amman, Fabian. University of Vienna. Institute for Theoretical Chemistry; Austria. Medical University of Vienna. Division of Cell and Developmental Biology; Austria
Fil: Linhartová, Irena. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Molecular Biology of Bacterial Pathogens; República Checa
Fil: Dienstbier, Ana. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Kumar, Dilip. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Držmíšek, Jakub. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
Fil: Hofacker, Ivo. University of Vienna. Institute for Theoretical Chemistry; Austria. University of Vienna. Faculty of Computer Science, Research Group Bioinformatics and Computational Biology; Austria
Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Večerek, Branislav. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
description Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143520
Petráčková, Denisa; Farman, Mariam R.; Amman, Fabian; Linhartová, Irena; Dienstbier, Ana; et al.; Transcriptional profiling of human macrophages during infection with Bordetella pertussis; Landes Bioscience; Rna Biology; 17; 5; 19-2-2020; 731-742
1547-6286
1555-8584
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143520
identifier_str_mv Petráčková, Denisa; Farman, Mariam R.; Amman, Fabian; Linhartová, Irena; Dienstbier, Ana; et al.; Transcriptional profiling of human macrophages during infection with Bordetella pertussis; Landes Bioscience; Rna Biology; 17; 5; 19-2-2020; 731-742
1547-6286
1555-8584
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/15476286.2020.1727694
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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