Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells

Autores
Albani, Clara Maria; Denegri, Guillermo María; Elissondo, María Celina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 μg/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 10(6) (100%) after 7 days of incubation. At day 7, thymol 5 μg/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 μg/ml, M. pulegium 10 μg/ml, and R. officinalis 10 μg/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth.
Facultad de Ciencias Veterinarias
Consejo Nacional de Investigaciones Científicas y Técnicas
Materia
Ciencias Veterinarias
Echinococcus granulosus
Larval cells
Essential oils
Mentha piperita
Mentha pulegium
Rosmarinus officinalis
Thymol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/98361
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/98361
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network_name_str SEDICI (UNLP)
spelling Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval CellsAlbani, Clara MariaDenegri, Guillermo MaríaElissondo, María CelinaCiencias VeterinariasEchinococcus granulosusLarval cellsEssential oilsMentha piperitaMentha pulegiumRosmarinus officinalisThymolHuman cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 μg/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 10(6) (100%) after 7 days of incubation. At day 7, thymol 5 μg/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 μg/ml, M. pulegium 10 μg/ml, and R. officinalis 10 μg/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth.Facultad de Ciencias VeterinariasConsejo Nacional de Investigaciones Científicas y Técnicas2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/98361enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/34859info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ipid/2014/746931/info:eu-repo/semantics/altIdentifier/issn/1687-708Xinfo:eu-repo/semantics/altIdentifier/doi/10.1155/2014/746931info:eu-repo/semantics/altIdentifier/hdl/11336/34859info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:12:59Zoai:sedici.unlp.edu.ar:10915/98361Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:12:59.902SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
title Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
spellingShingle Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
Albani, Clara Maria
Ciencias Veterinarias
Echinococcus granulosus
Larval cells
Essential oils
Mentha piperita
Mentha pulegium
Rosmarinus officinalis
Thymol
title_short Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
title_full Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
title_fullStr Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
title_full_unstemmed Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
title_sort Effect of Different Terpene-Containing Essential Oils on the Proliferation of <i>Echinococcus granulosus</i> Larval Cells
dc.creator.none.fl_str_mv Albani, Clara Maria
Denegri, Guillermo María
Elissondo, María Celina
author Albani, Clara Maria
author_facet Albani, Clara Maria
Denegri, Guillermo María
Elissondo, María Celina
author_role author
author2 Denegri, Guillermo María
Elissondo, María Celina
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Echinococcus granulosus
Larval cells
Essential oils
Mentha piperita
Mentha pulegium
Rosmarinus officinalis
Thymol
topic Ciencias Veterinarias
Echinococcus granulosus
Larval cells
Essential oils
Mentha piperita
Mentha pulegium
Rosmarinus officinalis
Thymol
dc.description.none.fl_txt_mv Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 μg/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 10(6) (100%) after 7 days of incubation. At day 7, thymol 5 μg/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 μg/ml, M. pulegium 10 μg/ml, and R. officinalis 10 μg/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth.
Facultad de Ciencias Veterinarias
Consejo Nacional de Investigaciones Científicas y Técnicas
description Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 μg/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 10(6) (100%) after 7 days of incubation. At day 7, thymol 5 μg/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 μg/ml, M. pulegium 10 μg/ml, and R. officinalis 10 μg/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/98361
url http://sedici.unlp.edu.ar/handle/10915/98361
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/34859
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ipid/2014/746931/
info:eu-repo/semantics/altIdentifier/issn/1687-708X
info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/746931
info:eu-repo/semantics/altIdentifier/hdl/11336/34859
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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