Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates

Autores
Iglesias, Néstor Gabriel; Gago Zachert, Selma Pérsida; Robledo Dobladez, Germán Ariel; Costa, Norma B.; Plata, Maria Inés; Vera, Osmar D.; Grau, Oscar; Semorile, Liliana Carmen
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the genetic variability of three genomic regions (p23, p25 and p27 genes) from 11 field Citrus tristeza virus isolates from the two main citrus growing areas of Argentina, a country where the most efficient vector of the virus, Toxoptera citricida, is present for decades. The pathogenicity of the isolates was determinated by biological indexing, single-strand conformation polymorphism analysis showed that most isolates contained high intra-isolate variability. Divergent sequence variants were detected in some isolates, suggesting re-infections of the field plants. Phylogenetic analysis of the predominant sequence variants of each isolate revealed similar grouping of isolates for genes p25 and p27. The analysis of p23 showed two groups contained the severe isolates. Our results showed a high intra-isolate sequence variability suggesting that re-infections could contribute to the observed variability and that the host can play an important role in the selection of the sequence variants present in these isolates.
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Naturales
Biología
Citrus tristeza virus
Toxoptera citricida
Single-strand conformation polymorphism
Nucleotide diversity
Mixed infections
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136099
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/136099
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolatesIglesias, Néstor GabrielGago Zachert, Selma PérsidaRobledo Dobladez, Germán ArielCosta, Norma B.Plata, Maria InésVera, Osmar D.Grau, OscarSemorile, Liliana CarmenCiencias NaturalesBiologíaCitrus tristeza virusToxoptera citricidaSingle-strand conformation polymorphismNucleotide diversityMixed infectionsWe studied the genetic variability of three genomic regions (p23, p25 and p27 genes) from 11 field Citrus tristeza virus isolates from the two main citrus growing areas of Argentina, a country where the most efficient vector of the virus, <i>Toxoptera citricida</i>, is present for decades. The pathogenicity of the isolates was determinated by biological indexing, single-strand conformation polymorphism analysis showed that most isolates contained high intra-isolate variability. Divergent sequence variants were detected in some isolates, suggesting re-infections of the field plants. Phylogenetic analysis of the predominant sequence variants of each isolate revealed similar grouping of isolates for genes p25 and p27. The analysis of p23 showed two groups contained the severe isolates. Our results showed a high intra-isolate sequence variability suggesting that re-infections could contribute to the observed variability and that the host can play an important role in the selection of the sequence variants present in these isolates.Instituto de Biotecnología y Biología Molecular2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf199-207http://sedici.unlp.edu.ar/handle/10915/136099enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-007-0169-xinfo:eu-repo/semantics/altIdentifier/pmid/17999168info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:57Zoai:sedici.unlp.edu.ar:10915/136099Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.271SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
title Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
spellingShingle Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
Iglesias, Néstor Gabriel
Ciencias Naturales
Biología
Citrus tristeza virus
Toxoptera citricida
Single-strand conformation polymorphism
Nucleotide diversity
Mixed infections
title_short Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
title_full Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
title_fullStr Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
title_full_unstemmed Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
title_sort Population structure of <i>Citrus tristeza virus</i> from field Argentinean isolates
dc.creator.none.fl_str_mv Iglesias, Néstor Gabriel
Gago Zachert, Selma Pérsida
Robledo Dobladez, Germán Ariel
Costa, Norma B.
Plata, Maria Inés
Vera, Osmar D.
Grau, Oscar
Semorile, Liliana Carmen
author Iglesias, Néstor Gabriel
author_facet Iglesias, Néstor Gabriel
Gago Zachert, Selma Pérsida
Robledo Dobladez, Germán Ariel
Costa, Norma B.
Plata, Maria Inés
Vera, Osmar D.
Grau, Oscar
Semorile, Liliana Carmen
author_role author
author2 Gago Zachert, Selma Pérsida
Robledo Dobladez, Germán Ariel
Costa, Norma B.
Plata, Maria Inés
Vera, Osmar D.
Grau, Oscar
Semorile, Liliana Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Biología
Citrus tristeza virus
Toxoptera citricida
Single-strand conformation polymorphism
Nucleotide diversity
Mixed infections
topic Ciencias Naturales
Biología
Citrus tristeza virus
Toxoptera citricida
Single-strand conformation polymorphism
Nucleotide diversity
Mixed infections
dc.description.none.fl_txt_mv We studied the genetic variability of three genomic regions (p23, p25 and p27 genes) from 11 field Citrus tristeza virus isolates from the two main citrus growing areas of Argentina, a country where the most efficient vector of the virus, <i>Toxoptera citricida</i>, is present for decades. The pathogenicity of the isolates was determinated by biological indexing, single-strand conformation polymorphism analysis showed that most isolates contained high intra-isolate variability. Divergent sequence variants were detected in some isolates, suggesting re-infections of the field plants. Phylogenetic analysis of the predominant sequence variants of each isolate revealed similar grouping of isolates for genes p25 and p27. The analysis of p23 showed two groups contained the severe isolates. Our results showed a high intra-isolate sequence variability suggesting that re-infections could contribute to the observed variability and that the host can play an important role in the selection of the sequence variants present in these isolates.
Instituto de Biotecnología y Biología Molecular
description We studied the genetic variability of three genomic regions (p23, p25 and p27 genes) from 11 field Citrus tristeza virus isolates from the two main citrus growing areas of Argentina, a country where the most efficient vector of the virus, <i>Toxoptera citricida</i>, is present for decades. The pathogenicity of the isolates was determinated by biological indexing, single-strand conformation polymorphism analysis showed that most isolates contained high intra-isolate variability. Divergent sequence variants were detected in some isolates, suggesting re-infections of the field plants. Phylogenetic analysis of the predominant sequence variants of each isolate revealed similar grouping of isolates for genes p25 and p27. The analysis of p23 showed two groups contained the severe isolates. Our results showed a high intra-isolate sequence variability suggesting that re-infections could contribute to the observed variability and that the host can play an important role in the selection of the sequence variants present in these isolates.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136099
url http://sedici.unlp.edu.ar/handle/10915/136099
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/issn/1572-994X
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-007-0169-x
info:eu-repo/semantics/altIdentifier/pmid/17999168
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
199-207
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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