Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
- Autores
- Tarocco, Federico; Lecuona, Roberto E.; Couto, Alicia S.; Arcas, Jorge Alfredo
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (aw), pH, and fermentation time for Beauveria bassiana conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and aw=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and aw 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.
Centro de Investigación y Desarrollo en Fermentaciones Industriales - Materia
-
Ciencias Exactas
Química
Polyol
Erythritol
Arabitol
Conidium Production
Solid Substrate Fermentation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/144410
Ver los metadatos del registro completo
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Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodologyTarocco, FedericoLecuona, Roberto E.Couto, Alicia S.Arcas, Jorge AlfredoCiencias ExactasQuímicaPolyolErythritolArabitolConidium ProductionSolid Substrate FermentationEntomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.Centro de Investigación y Desarrollo en Fermentaciones Industriales2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf481-488http://sedici.unlp.edu.ar/handle/10915/144410enginfo:eu-repo/semantics/altIdentifier/issn/0175-7598info:eu-repo/semantics/altIdentifier/issn/1432-0614info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-005-1901-xinfo:eu-repo/semantics/altIdentifier/pmid/15731900info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/144410Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.196SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
title |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
spellingShingle |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology Tarocco, Federico Ciencias Exactas Química Polyol Erythritol Arabitol Conidium Production Solid Substrate Fermentation |
title_short |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
title_full |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
title_fullStr |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
title_full_unstemmed |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
title_sort |
Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology |
dc.creator.none.fl_str_mv |
Tarocco, Federico Lecuona, Roberto E. Couto, Alicia S. Arcas, Jorge Alfredo |
author |
Tarocco, Federico |
author_facet |
Tarocco, Federico Lecuona, Roberto E. Couto, Alicia S. Arcas, Jorge Alfredo |
author_role |
author |
author2 |
Lecuona, Roberto E. Couto, Alicia S. Arcas, Jorge Alfredo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Química Polyol Erythritol Arabitol Conidium Production Solid Substrate Fermentation |
topic |
Ciencias Exactas Química Polyol Erythritol Arabitol Conidium Production Solid Substrate Fermentation |
dc.description.none.fl_txt_mv |
Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed. Centro de Investigación y Desarrollo en Fermentaciones Industriales |
description |
Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/144410 |
url |
http://sedici.unlp.edu.ar/handle/10915/144410 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0175-7598 info:eu-repo/semantics/altIdentifier/issn/1432-0614 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-005-1901-x info:eu-repo/semantics/altIdentifier/pmid/15731900 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 481-488 |
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