Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology

Autores
Tarocco, Federico; Lecuona, Roberto E.; Couto, Alicia S.; Arcas, Jorge Alfredo
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (aw), pH, and fermentation time for Beauveria bassiana conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and aw=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and aw 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Química
Polyol
Erythritol
Arabitol
Conidium Production
Solid Substrate Fermentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/144410

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodologyTarocco, FedericoLecuona, Roberto E.Couto, Alicia S.Arcas, Jorge AlfredoCiencias ExactasQuímicaPolyolErythritolArabitolConidium ProductionSolid Substrate FermentationEntomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.Centro de Investigación y Desarrollo en Fermentaciones Industriales2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf481-488http://sedici.unlp.edu.ar/handle/10915/144410enginfo:eu-repo/semantics/altIdentifier/issn/0175-7598info:eu-repo/semantics/altIdentifier/issn/1432-0614info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-005-1901-xinfo:eu-repo/semantics/altIdentifier/pmid/15731900info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/144410Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.196SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
title Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
spellingShingle Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
Tarocco, Federico
Ciencias Exactas
Química
Polyol
Erythritol
Arabitol
Conidium Production
Solid Substrate Fermentation
title_short Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
title_full Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
title_fullStr Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
title_full_unstemmed Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
title_sort Optimization of erythritol and glycerol accumulation in conidia of <i>Beauveria bassiana</i> by solid-state fermentation, using response surface methodology
dc.creator.none.fl_str_mv Tarocco, Federico
Lecuona, Roberto E.
Couto, Alicia S.
Arcas, Jorge Alfredo
author Tarocco, Federico
author_facet Tarocco, Federico
Lecuona, Roberto E.
Couto, Alicia S.
Arcas, Jorge Alfredo
author_role author
author2 Lecuona, Roberto E.
Couto, Alicia S.
Arcas, Jorge Alfredo
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Polyol
Erythritol
Arabitol
Conidium Production
Solid Substrate Fermentation
topic Ciencias Exactas
Química
Polyol
Erythritol
Arabitol
Conidium Production
Solid Substrate Fermentation
dc.description.none.fl_txt_mv Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a<sub>w</sub>), pH, and fermentation time for <i>Beauveria bassiana</i> conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5–6 and a<sub>w</sub>=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a<sub>w</sub> 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/144410
url http://sedici.unlp.edu.ar/handle/10915/144410
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0175-7598
info:eu-repo/semantics/altIdentifier/issn/1432-0614
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-005-1901-x
info:eu-repo/semantics/altIdentifier/pmid/15731900
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
481-488
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instname:Universidad Nacional de La Plata
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instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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