Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi

Autores
Pedrini, Nicolás; Mijailovsky, Sergio Javier; Girotti, Juan Roberto; Stariolo, Raúl; Cardozo, Rubén M.; Gentile, Alberto; Juárez, Marta Patricia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern Argentina, Bolivia, and Paraguay - are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. Methodology and Findings: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 μm and 17.8±5.4 μm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. Conclusions: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs.
Facultad de Ciencias Médicas
Materia
Ciencias Médicas
insecticide
Chagas disease
insect
microbiology
parasitology
physiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/40293

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spelling Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungiPedrini, NicolásMijailovsky, Sergio JavierGirotti, Juan RobertoStariolo, RaúlCardozo, Rubén M.Gentile, AlbertoJuárez, Marta PatriciaCiencias MédicasinsecticideChagas diseaseinsectmicrobiologyparasitologyphysiologyBackground: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern Argentina, Bolivia, and Paraguay - are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. Methodology and Findings: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 μm and 17.8±5.4 μm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. Conclusions: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs.Facultad de Ciencias Médicas2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/40293enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosntds.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pntd.0000434&representation=PDFinfo:eu-repo/semantics/altIdentifier/issn/1935-2727info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0000434info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T10:57:09Zoai:sedici.unlp.edu.ar:10915/40293Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 10:57:09.335SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
title Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
spellingShingle Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
Pedrini, Nicolás
Ciencias Médicas
insecticide
Chagas disease
insect
microbiology
parasitology
physiology
title_short Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
title_full Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
title_fullStr Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
title_full_unstemmed Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
title_sort Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
dc.creator.none.fl_str_mv Pedrini, Nicolás
Mijailovsky, Sergio Javier
Girotti, Juan Roberto
Stariolo, Raúl
Cardozo, Rubén M.
Gentile, Alberto
Juárez, Marta Patricia
author Pedrini, Nicolás
author_facet Pedrini, Nicolás
Mijailovsky, Sergio Javier
Girotti, Juan Roberto
Stariolo, Raúl
Cardozo, Rubén M.
Gentile, Alberto
Juárez, Marta Patricia
author_role author
author2 Mijailovsky, Sergio Javier
Girotti, Juan Roberto
Stariolo, Raúl
Cardozo, Rubén M.
Gentile, Alberto
Juárez, Marta Patricia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
insecticide
Chagas disease
insect
microbiology
parasitology
physiology
topic Ciencias Médicas
insecticide
Chagas disease
insect
microbiology
parasitology
physiology
dc.description.none.fl_txt_mv Background: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern Argentina, Bolivia, and Paraguay - are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. Methodology and Findings: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 μm and 17.8±5.4 μm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. Conclusions: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs.
Facultad de Ciencias Médicas
description Background: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern Argentina, Bolivia, and Paraguay - are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. Methodology and Findings: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 μm and 17.8±5.4 μm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. Conclusions: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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