Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation P...

Autores
Guanuco, Andrea Paola; Davies, Carolina; Poma, Hugo Ramiro; Gentile, Alberto Gerónimo; Cardozo, Rubén Marino
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Populations of Triatomas infestans with different susceptibility to pyrethroid insecticides were reported to have distinct evolutionary and epidemiological characteristics. We aimed at evaluating metacyclogenesis and parasite load as measures of vector competence and feeding/defecation patterns as vectorial capacity estimates of a group of resistant (“R”) and susceptible (“S”) T. infestans. Third instar nymphs of each group were fed on mice infected with Trypanosoma cruzi (Tulahuén strain, DTU VI). Parasite concentration in blood was quantified by real-time PCR (qPCR) for each mouse. The time at which feeding started, the span of feeding, the volume of blood consumed, and the time taken to defecate were measured. At 30 days post-feeding, feces were analyzed in parallel by optical microscopy (percentage of metacyclic trypomastigotes, % MT), and qPCR (total T. cruzi DNA). The ratio of parasites consumed/defecated by nymphs of each group was used to estimate the parasites’ survival and multiplication inside the triatomines’ gut. It was estimated that for each blood trypomastigote consumed, 6.6 parasites were obtained in the feces of “R” nymphs, and 7.9 in “S”. “R” nymphs consumed a higher volume of blood, had lower % MT in their feces (lower vectorial competence), and took longer to defecate (lower vectorial capacity) than “S”.
Fil: Guanuco, Andrea Paola. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Davies, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Cardozo, Rubén Marino. Provincia de Salta. Ministerio de Salud Pública; Argentina
Materia
TRIATOMINAE
CHAGAS DISEASE
INSECTICIDE RESISTANCE
VECTOR CAPACITY
VECTOR COMPETENCE
QPCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211046
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/211046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite LoadGuanuco, Andrea PaolaDavies, CarolinaPoma, Hugo RamiroGentile, Alberto GerónimoCardozo, Rubén MarinoTRIATOMINAECHAGAS DISEASEINSECTICIDE RESISTANCEVECTOR CAPACITYVECTOR COMPETENCEQPCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Populations of Triatomas infestans with different susceptibility to pyrethroid insecticides were reported to have distinct evolutionary and epidemiological characteristics. We aimed at evaluating metacyclogenesis and parasite load as measures of vector competence and feeding/defecation patterns as vectorial capacity estimates of a group of resistant (“R”) and susceptible (“S”) T. infestans. Third instar nymphs of each group were fed on mice infected with Trypanosoma cruzi (Tulahuén strain, DTU VI). Parasite concentration in blood was quantified by real-time PCR (qPCR) for each mouse. The time at which feeding started, the span of feeding, the volume of blood consumed, and the time taken to defecate were measured. At 30 days post-feeding, feces were analyzed in parallel by optical microscopy (percentage of metacyclic trypomastigotes, % MT), and qPCR (total T. cruzi DNA). The ratio of parasites consumed/defecated by nymphs of each group was used to estimate the parasites’ survival and multiplication inside the triatomines’ gut. It was estimated that for each blood trypomastigote consumed, 6.6 parasites were obtained in the feces of “R” nymphs, and 7.9 in “S”. “R” nymphs consumed a higher volume of blood, had lower % MT in their feces (lower vectorial competence), and took longer to defecate (lower vectorial capacity) than “S”.Fil: Guanuco, Andrea Paola. Provincia de Salta. Ministerio de Salud Pública; ArgentinaFil: Davies, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; ArgentinaFil: Cardozo, Rubén Marino. Provincia de Salta. Ministerio de Salud Pública; ArgentinaMultidisciplinary Digital Publishing Institute2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211046Guanuco, Andrea Paola; Davies, Carolina; Poma, Hugo Ramiro; Gentile, Alberto Gerónimo; Cardozo, Rubén Marino; Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load; Multidisciplinary Digital Publishing Institute; Parasitología; 2; 10-2022; 255-2652673-6772CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/parasitologia2040022info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6772/2/4/22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:20Zoai:ri.conicet.gov.ar:11336/211046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:21.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
title Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
spellingShingle Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
Guanuco, Andrea Paola
TRIATOMINAE
CHAGAS DISEASE
INSECTICIDE RESISTANCE
VECTOR CAPACITY
VECTOR COMPETENCE
QPCR
title_short Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
title_full Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
title_fullStr Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
title_full_unstemmed Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
title_sort Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load
dc.creator.none.fl_str_mv Guanuco, Andrea Paola
Davies, Carolina
Poma, Hugo Ramiro
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author Guanuco, Andrea Paola
author_facet Guanuco, Andrea Paola
Davies, Carolina
Poma, Hugo Ramiro
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author_role author
author2 Davies, Carolina
Poma, Hugo Ramiro
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author2_role author
author
author
author
dc.subject.none.fl_str_mv TRIATOMINAE
CHAGAS DISEASE
INSECTICIDE RESISTANCE
VECTOR CAPACITY
VECTOR COMPETENCE
QPCR
topic TRIATOMINAE
CHAGAS DISEASE
INSECTICIDE RESISTANCE
VECTOR CAPACITY
VECTOR COMPETENCE
QPCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Populations of Triatomas infestans with different susceptibility to pyrethroid insecticides were reported to have distinct evolutionary and epidemiological characteristics. We aimed at evaluating metacyclogenesis and parasite load as measures of vector competence and feeding/defecation patterns as vectorial capacity estimates of a group of resistant (“R”) and susceptible (“S”) T. infestans. Third instar nymphs of each group were fed on mice infected with Trypanosoma cruzi (Tulahuén strain, DTU VI). Parasite concentration in blood was quantified by real-time PCR (qPCR) for each mouse. The time at which feeding started, the span of feeding, the volume of blood consumed, and the time taken to defecate were measured. At 30 days post-feeding, feces were analyzed in parallel by optical microscopy (percentage of metacyclic trypomastigotes, % MT), and qPCR (total T. cruzi DNA). The ratio of parasites consumed/defecated by nymphs of each group was used to estimate the parasites’ survival and multiplication inside the triatomines’ gut. It was estimated that for each blood trypomastigote consumed, 6.6 parasites were obtained in the feces of “R” nymphs, and 7.9 in “S”. “R” nymphs consumed a higher volume of blood, had lower % MT in their feces (lower vectorial competence), and took longer to defecate (lower vectorial capacity) than “S”.
Fil: Guanuco, Andrea Paola. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Davies, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Cardozo, Rubén Marino. Provincia de Salta. Ministerio de Salud Pública; Argentina
description Populations of Triatomas infestans with different susceptibility to pyrethroid insecticides were reported to have distinct evolutionary and epidemiological characteristics. We aimed at evaluating metacyclogenesis and parasite load as measures of vector competence and feeding/defecation patterns as vectorial capacity estimates of a group of resistant (“R”) and susceptible (“S”) T. infestans. Third instar nymphs of each group were fed on mice infected with Trypanosoma cruzi (Tulahuén strain, DTU VI). Parasite concentration in blood was quantified by real-time PCR (qPCR) for each mouse. The time at which feeding started, the span of feeding, the volume of blood consumed, and the time taken to defecate were measured. At 30 days post-feeding, feces were analyzed in parallel by optical microscopy (percentage of metacyclic trypomastigotes, % MT), and qPCR (total T. cruzi DNA). The ratio of parasites consumed/defecated by nymphs of each group was used to estimate the parasites’ survival and multiplication inside the triatomines’ gut. It was estimated that for each blood trypomastigote consumed, 6.6 parasites were obtained in the feces of “R” nymphs, and 7.9 in “S”. “R” nymphs consumed a higher volume of blood, had lower % MT in their feces (lower vectorial competence), and took longer to defecate (lower vectorial capacity) than “S”.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211046
Guanuco, Andrea Paola; Davies, Carolina; Poma, Hugo Ramiro; Gentile, Alberto Gerónimo; Cardozo, Rubén Marino; Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load; Multidisciplinary Digital Publishing Institute; Parasitología; 2; 10-2022; 255-265
2673-6772
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211046
identifier_str_mv Guanuco, Andrea Paola; Davies, Carolina; Poma, Hugo Ramiro; Gentile, Alberto Gerónimo; Cardozo, Rubén Marino; Pyrethroid-Resistant and Susceptible Triatoma infestans (Klug, 1834) (Hemiptera, Triatominae): Analysis of Their Vectorial Characteristics by Metacyclogenesis, Feeding/Defecation Patterns, and Parasite Load; Multidisciplinary Digital Publishing Institute; Parasitología; 2; 10-2022; 255-265
2673-6772
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/parasitologia2040022
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6772/2/4/22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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