Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds

Autores
Sosa, Ayelen Morena; Cottet, Celeste; Berin, Belén; Martínez, Luis; Peltzer, Mercedes; Prieto, María Jimena; Martinez, Carolina Soledad
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound.
Instituto Multidisciplinario de Biología Celular
Materia
Biología
Química
gelatin films
silver nanoparticles
o/w emulsions
characterization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/181748
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/181748
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive CompoundsSosa, Ayelen MorenaCottet, CelesteBerin, BelénMartínez, LuisPeltzer, MercedesPrieto, María JimenaMartinez, Carolina SoledadBiologíaQuímicagelatin filmssilver nanoparticleso/w emulsionscharacterizationBackground: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound.Instituto Multidisciplinario de Biología Celular2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/181748enginfo:eu-repo/semantics/altIdentifier/issn/1999-4923info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics17030357info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:21:24Zoai:sedici.unlp.edu.ar:10915/181748Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:21:25.369SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
title Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
spellingShingle Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
Sosa, Ayelen Morena
Biología
Química
gelatin films
silver nanoparticles
o/w emulsions
characterization
title_short Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
title_full Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
title_fullStr Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
title_full_unstemmed Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
title_sort Development of Films for Wound Healing Based on Gelatin and Oil/Water Emulsions as Carriers of Bioactive Compounds
dc.creator.none.fl_str_mv Sosa, Ayelen Morena
Cottet, Celeste
Berin, Belén
Martínez, Luis
Peltzer, Mercedes
Prieto, María Jimena
Martinez, Carolina Soledad
author Sosa, Ayelen Morena
author_facet Sosa, Ayelen Morena
Cottet, Celeste
Berin, Belén
Martínez, Luis
Peltzer, Mercedes
Prieto, María Jimena
Martinez, Carolina Soledad
author_role author
author2 Cottet, Celeste
Berin, Belén
Martínez, Luis
Peltzer, Mercedes
Prieto, María Jimena
Martinez, Carolina Soledad
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Química
gelatin films
silver nanoparticles
o/w emulsions
characterization
topic Biología
Química
gelatin films
silver nanoparticles
o/w emulsions
characterization
dc.description.none.fl_txt_mv Background: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound.
Instituto Multidisciplinario de Biología Celular
description Background: Natural biopolymeric matrices for developing dressings have been extensively studied, as they may exhibit beneficial properties for wound healing. Gelatin possesses promising structural and physicochemical properties for incorporating active compounds (ACs). O/W emulsions are an alternative delivery system for AC with different properties and solubilities, promoting wound healing. Objective: This study aimed to develop gelatin films by adding silver nanoparticles and healing agents encapsulated in an O/W emulsion to treat skin wounds. Methods: A film-forming dispersion was prepared using gelatin and glycerol as a plasticizer, and films were obtained using the casting technique. Emulsions with ACs (EAs) and without ACs (ECs) were incorporated into the films. The formulations were analyzed by FESEM and characterized for their mechanical, thermal, and swelling properties, as well as their water vapor permeability. Results: The results showed that films with a higher amount of emulsion exhibited greater structural rigidity and lower permeability, while films with lower amounts of emulsion demonstrated more elasticity and higher permeability. General and organ-specific toxicity were evaluated in zebrafish larvae. The films showed no lethal or sub-lethal effects on the morphology or activity of the brain, heart, and liver. Conclusions: The active films developed could provide stable support and a safe delivery system for active compounds to treat skin lesions, minimizing the risk of infection and the need to heal a wound.
publishDate 2025
dc.date.none.fl_str_mv 2025-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/181748
url http://sedici.unlp.edu.ar/handle/10915/181748
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1999-4923
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics17030357
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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