Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli

Autores
Cáceres, María Emilia; Lavayen, Silvina; Zotta, Claudio Marcelo; Montero, David; Vidal, Roberto; Etcheverría, Analía Inés; Padola, Nora Lía
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is the most prevalent serotype associated with severe diseases worldwide. Biofilms by STEC O157:H7 constitute a high risk to public health and the food industry since they allow cross-contamination of surfaces and the consequent transmission to humans. This study aimed to detect the presence of adhesins genotypically and determine the ability to form biofilm and the curli expression in a collection of 30 O157:H7 strains from healthy cattle and human cases. The efa1, iha, fimCD, ehaA, lpfA1-3, and lpfA2-2 genes were detected in all strains; cah was frequently detected in strains isolated from humans (16/20), and agn43 was the least prevalent gene (3/30). All strains could form a biofilm, although those isolated from cattle were the most biofilm-formers. The curli-negative phenotype was the most prevalent phenotype observed at 37 °C and room temperature. The association between curli production and biofilm formation could not be determined, but the highest proportion of curli-positive strains at room temperature were strong biofilm-formers. These results highlight the possibility of the persistence of STEC O157:H7 in environmental conditions and food processing facilities, increasing the risk of contamination or infection.
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Lavayen, Silvina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Zotta, Claudio Marcelo. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Montero, David. Universidad de Chile; Chile
Fil: Vidal, Roberto. Universidad de Chile; Chile
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
BIOFILM FORMATION
CURLI FIMBRIAE
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN-PRODUCING ESCHERICHIA COLI
SOURCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215513
Acceder
id CONICETDig_05ed1dcfa43106393608da9bb8708f99
oai_identifier_str oai:ri.conicet.gov.ar:11336/215513
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coliCáceres, María EmiliaLavayen, SilvinaZotta, Claudio MarceloMontero, DavidVidal, RobertoEtcheverría, Analía InésPadola, Nora LíaBIOFILM FORMATIONCURLI FIMBRIAEHEMOLYTIC UREMIC SYNDROMESHIGA TOXIN-PRODUCING ESCHERICHIA COLISOURCEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Shiga toxin-producing Escherichia coli (STEC) O157:H7 is the most prevalent serotype associated with severe diseases worldwide. Biofilms by STEC O157:H7 constitute a high risk to public health and the food industry since they allow cross-contamination of surfaces and the consequent transmission to humans. This study aimed to detect the presence of adhesins genotypically and determine the ability to form biofilm and the curli expression in a collection of 30 O157:H7 strains from healthy cattle and human cases. The efa1, iha, fimCD, ehaA, lpfA1-3, and lpfA2-2 genes were detected in all strains; cah was frequently detected in strains isolated from humans (16/20), and agn43 was the least prevalent gene (3/30). All strains could form a biofilm, although those isolated from cattle were the most biofilm-formers. The curli-negative phenotype was the most prevalent phenotype observed at 37 °C and room temperature. The association between curli production and biofilm formation could not be determined, but the highest proportion of curli-positive strains at room temperature were strong biofilm-formers. These results highlight the possibility of the persistence of STEC O157:H7 in environmental conditions and food processing facilities, increasing the risk of contamination or infection.Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; ArgentinaFil: Lavayen, Silvina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Zotta, Claudio Marcelo. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Montero, David. Universidad de Chile; ChileFil: Vidal, Roberto. Universidad de Chile; ChileFil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaDovepress2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215513Cáceres, María Emilia; Lavayen, Silvina; Zotta, Claudio Marcelo; Montero, David; Vidal, Roberto; et al.; Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli; Dovepress; Veterinary Medicine Reports; 1; 603; 2-2022; 1-92230-2034CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.55085/vmr.2022.603info:eu-repo/semantics/altIdentifier/url/https://mediterraneanjournals.com/index.php/vmr/article/view/603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:39Zoai:ri.conicet.gov.ar:11336/215513instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:40.186CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
title Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
spellingShingle Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
Cáceres, María Emilia
BIOFILM FORMATION
CURLI FIMBRIAE
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN-PRODUCING ESCHERICHIA COLI
SOURCES
title_short Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
title_full Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
title_fullStr Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
title_full_unstemmed Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
title_sort Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli
dc.creator.none.fl_str_mv Cáceres, María Emilia
Lavayen, Silvina
Zotta, Claudio Marcelo
Montero, David
Vidal, Roberto
Etcheverría, Analía Inés
Padola, Nora Lía
author Cáceres, María Emilia
author_facet Cáceres, María Emilia
Lavayen, Silvina
Zotta, Claudio Marcelo
Montero, David
Vidal, Roberto
Etcheverría, Analía Inés
Padola, Nora Lía
author_role author
author2 Lavayen, Silvina
Zotta, Claudio Marcelo
Montero, David
Vidal, Roberto
Etcheverría, Analía Inés
Padola, Nora Lía
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOFILM FORMATION
CURLI FIMBRIAE
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN-PRODUCING ESCHERICHIA COLI
SOURCES
topic BIOFILM FORMATION
CURLI FIMBRIAE
HEMOLYTIC UREMIC SYNDROME
SHIGA TOXIN-PRODUCING ESCHERICHIA COLI
SOURCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Shiga toxin-producing Escherichia coli (STEC) O157:H7 is the most prevalent serotype associated with severe diseases worldwide. Biofilms by STEC O157:H7 constitute a high risk to public health and the food industry since they allow cross-contamination of surfaces and the consequent transmission to humans. This study aimed to detect the presence of adhesins genotypically and determine the ability to form biofilm and the curli expression in a collection of 30 O157:H7 strains from healthy cattle and human cases. The efa1, iha, fimCD, ehaA, lpfA1-3, and lpfA2-2 genes were detected in all strains; cah was frequently detected in strains isolated from humans (16/20), and agn43 was the least prevalent gene (3/30). All strains could form a biofilm, although those isolated from cattle were the most biofilm-formers. The curli-negative phenotype was the most prevalent phenotype observed at 37 °C and room temperature. The association between curli production and biofilm formation could not be determined, but the highest proportion of curli-positive strains at room temperature were strong biofilm-formers. These results highlight the possibility of the persistence of STEC O157:H7 in environmental conditions and food processing facilities, increasing the risk of contamination or infection.
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Lavayen, Silvina. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Zotta, Claudio Marcelo. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Montero, David. Universidad de Chile; Chile
Fil: Vidal, Roberto. Universidad de Chile; Chile
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description Shiga toxin-producing Escherichia coli (STEC) O157:H7 is the most prevalent serotype associated with severe diseases worldwide. Biofilms by STEC O157:H7 constitute a high risk to public health and the food industry since they allow cross-contamination of surfaces and the consequent transmission to humans. This study aimed to detect the presence of adhesins genotypically and determine the ability to form biofilm and the curli expression in a collection of 30 O157:H7 strains from healthy cattle and human cases. The efa1, iha, fimCD, ehaA, lpfA1-3, and lpfA2-2 genes were detected in all strains; cah was frequently detected in strains isolated from humans (16/20), and agn43 was the least prevalent gene (3/30). All strains could form a biofilm, although those isolated from cattle were the most biofilm-formers. The curli-negative phenotype was the most prevalent phenotype observed at 37 °C and room temperature. The association between curli production and biofilm formation could not be determined, but the highest proportion of curli-positive strains at room temperature were strong biofilm-formers. These results highlight the possibility of the persistence of STEC O157:H7 in environmental conditions and food processing facilities, increasing the risk of contamination or infection.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215513
Cáceres, María Emilia; Lavayen, Silvina; Zotta, Claudio Marcelo; Montero, David; Vidal, Roberto; et al.; Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli; Dovepress; Veterinary Medicine Reports; 1; 603; 2-2022; 1-9
2230-2034
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215513
identifier_str_mv Cáceres, María Emilia; Lavayen, Silvina; Zotta, Claudio Marcelo; Montero, David; Vidal, Roberto; et al.; Phenotypic and Genotypic Determination of Biofilm Formation in Shiga Toxin-Producing Escherichia coli; Dovepress; Veterinary Medicine Reports; 1; 603; 2-2022; 1-9
2230-2034
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.55085/vmr.2022.603
info:eu-repo/semantics/altIdentifier/url/https://mediterraneanjournals.com/index.php/vmr/article/view/603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Dovepress
publisher.none.fl_str_mv Dovepress
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083304682422272
score 13.22299

Publicaciones similares