Role of type IV collagen in prolactin release from anterior pituitaries of male rats

Autores
Diaz, Emilce S.; Rettori, Valeria; Suescun, María Olga; Lustig, Livia; McCann, Samuel M.; Denduchis, Berta
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We previously demonstrated that laminin, a component of basement membranes, modulates pituitary hormone secretion. In the present study, we evaluated the effect of type IV collagen, another component of this membrane, on the release of prolactin (PRL) by anterior pituitary gland from adult male rats. Hemipituitaries were incubated for 3 h with type IV collagen or antibodies against it and PRL release was studied. Rabbit IgG to type IV collagen at concentrations of 10⁻⁷ - 10⁻⁵ M had a significant stimulatory effect on PRL release, in comparison to normal rabbit serum IgG or medium alone used as controls. Type IV collagen induced a significant inhibitory effect on basal release of PRL at a concentration of 30 µg/mL. A slight decrease in PRL release was detected in thyrotropin-releasing hormone-stimulated hemipituitaries incubated with type IV collagen at all concentrations used. These results suggest that type IV collagen, similar to laminin-1, modulates PRL released from hemipituitaries, in vitro.
Instituto Multidisciplinario de Biología Celular
Materia
Ciencias Médicas
Biología
type IV collagen
prolactin
anterior pituitary
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/146458
Acceder
id SEDICI_a762cbfbee293b64b04af540bcaec97a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/146458
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Role of type IV collagen in prolactin release from anterior pituitaries of male ratsDiaz, Emilce S.Rettori, ValeriaSuescun, María OlgaLustig, LiviaMcCann, Samuel M.Denduchis, BertaCiencias MédicasBiologíatype IV collagenprolactinanterior pituitaryWe previously demonstrated that laminin, a component of basement membranes, modulates pituitary hormone secretion. In the present study, we evaluated the effect of type IV collagen, another component of this membrane, on the release of prolactin (PRL) by anterior pituitary gland from adult male rats. Hemipituitaries were incubated for 3 h with type IV collagen or antibodies against it and PRL release was studied. Rabbit IgG to type IV collagen at concentrations of 10⁻⁷ - 10⁻⁵ M had a significant stimulatory effect on PRL release, in comparison to normal rabbit serum IgG or medium alone used as controls. Type IV collagen induced a significant inhibitory effect on basal release of PRL at a concentration of 30 µg/mL. A slight decrease in PRL release was detected in thyrotropin-releasing hormone-stimulated hemipituitaries incubated with type IV collagen at all concentrations used. These results suggest that type IV collagen, similar to laminin-1, modulates PRL released from hemipituitaries, in vitro.Instituto Multidisciplinario de Biología Celular2002-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf185-189http://sedici.unlp.edu.ar/handle/10915/146458enginfo:eu-repo/semantics/altIdentifier/issn/1355-008Xinfo:eu-repo/semantics/altIdentifier/issn/0969-711Xinfo:eu-repo/semantics/altIdentifier/doi/10.1385/endo:18:2:185info:eu-repo/semantics/altIdentifier/pmid/12374467info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/146458Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.167SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Role of type IV collagen in prolactin release from anterior pituitaries of male rats
title Role of type IV collagen in prolactin release from anterior pituitaries of male rats
spellingShingle Role of type IV collagen in prolactin release from anterior pituitaries of male rats
Diaz, Emilce S.
Ciencias Médicas
Biología
type IV collagen
prolactin
anterior pituitary
title_short Role of type IV collagen in prolactin release from anterior pituitaries of male rats
title_full Role of type IV collagen in prolactin release from anterior pituitaries of male rats
title_fullStr Role of type IV collagen in prolactin release from anterior pituitaries of male rats
title_full_unstemmed Role of type IV collagen in prolactin release from anterior pituitaries of male rats
title_sort Role of type IV collagen in prolactin release from anterior pituitaries of male rats
dc.creator.none.fl_str_mv Diaz, Emilce S.
Rettori, Valeria
Suescun, María Olga
Lustig, Livia
McCann, Samuel M.
Denduchis, Berta
author Diaz, Emilce S.
author_facet Diaz, Emilce S.
Rettori, Valeria
Suescun, María Olga
Lustig, Livia
McCann, Samuel M.
Denduchis, Berta
author_role author
author2 Rettori, Valeria
Suescun, María Olga
Lustig, Livia
McCann, Samuel M.
Denduchis, Berta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Biología
type IV collagen
prolactin
anterior pituitary
topic Ciencias Médicas
Biología
type IV collagen
prolactin
anterior pituitary
dc.description.none.fl_txt_mv We previously demonstrated that laminin, a component of basement membranes, modulates pituitary hormone secretion. In the present study, we evaluated the effect of type IV collagen, another component of this membrane, on the release of prolactin (PRL) by anterior pituitary gland from adult male rats. Hemipituitaries were incubated for 3 h with type IV collagen or antibodies against it and PRL release was studied. Rabbit IgG to type IV collagen at concentrations of 10⁻⁷ - 10⁻⁵ M had a significant stimulatory effect on PRL release, in comparison to normal rabbit serum IgG or medium alone used as controls. Type IV collagen induced a significant inhibitory effect on basal release of PRL at a concentration of 30 µg/mL. A slight decrease in PRL release was detected in thyrotropin-releasing hormone-stimulated hemipituitaries incubated with type IV collagen at all concentrations used. These results suggest that type IV collagen, similar to laminin-1, modulates PRL released from hemipituitaries, in vitro.
Instituto Multidisciplinario de Biología Celular
description We previously demonstrated that laminin, a component of basement membranes, modulates pituitary hormone secretion. In the present study, we evaluated the effect of type IV collagen, another component of this membrane, on the release of prolactin (PRL) by anterior pituitary gland from adult male rats. Hemipituitaries were incubated for 3 h with type IV collagen or antibodies against it and PRL release was studied. Rabbit IgG to type IV collagen at concentrations of 10⁻⁷ - 10⁻⁵ M had a significant stimulatory effect on PRL release, in comparison to normal rabbit serum IgG or medium alone used as controls. Type IV collagen induced a significant inhibitory effect on basal release of PRL at a concentration of 30 µg/mL. A slight decrease in PRL release was detected in thyrotropin-releasing hormone-stimulated hemipituitaries incubated with type IV collagen at all concentrations used. These results suggest that type IV collagen, similar to laminin-1, modulates PRL released from hemipituitaries, in vitro.
publishDate 2002
dc.date.none.fl_str_mv 2002-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/146458
url http://sedici.unlp.edu.ar/handle/10915/146458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1355-008X
info:eu-repo/semantics/altIdentifier/issn/0969-711X
info:eu-repo/semantics/altIdentifier/doi/10.1385/endo:18:2:185
info:eu-repo/semantics/altIdentifier/pmid/12374467
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
185-189
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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