In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows

Autores
Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Verónica; Bandoni, Arnaldo Luis; Marchetti, María Laura; Mestorino, Olga Nora
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/153603

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network_name_str SEDICI (UNLP)
spelling In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cowsBuldain, Daniel CornelioGortari Castillo, LihuelBuchamer, Andrea VerónicaBandoni, Arnaldo LuisMarchetti, María LauraMestorino, Olga NoraCiencias VeterinariasMelaleuca armillarisessential oilerythromycinsynergismStaphylococcus aureusmastitisStaphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.Facultad de Ciencias Veterinarias2022-11-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/153603enginfo:eu-repo/semantics/altIdentifier/issn/2297-1769info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2022.1005616info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:11:44Zoai:sedici.unlp.edu.ar:10915/153603Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:11:44.991SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
spellingShingle In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
Buldain, Daniel Cornelio
Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
title_short In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_full In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_fullStr In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_full_unstemmed In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_sort In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
dc.creator.none.fl_str_mv Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
author Buldain, Daniel Cornelio
author_facet Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
author_role author
author2 Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
topic Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
dc.description.none.fl_txt_mv Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
Facultad de Ciencias Veterinarias
description Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/153603
url http://sedici.unlp.edu.ar/handle/10915/153603
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2297-1769
info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2022.1005616
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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