In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
- Autores
- Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Verónica; Bandoni, Arnaldo Luis; Marchetti, María Laura; Mestorino, Olga Nora
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/153603
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In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cowsBuldain, Daniel CornelioGortari Castillo, LihuelBuchamer, Andrea VerónicaBandoni, Arnaldo LuisMarchetti, María LauraMestorino, Olga NoraCiencias VeterinariasMelaleuca armillarisessential oilerythromycinsynergismStaphylococcus aureusmastitisStaphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.Facultad de Ciencias Veterinarias2022-11-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/153603enginfo:eu-repo/semantics/altIdentifier/issn/2297-1769info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2022.1005616info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:11:44Zoai:sedici.unlp.edu.ar:10915/153603Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:11:44.991SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
title |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
spellingShingle |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows Buldain, Daniel Cornelio Ciencias Veterinarias Melaleuca armillaris essential oil erythromycin synergism Staphylococcus aureus mastitis |
title_short |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
title_full |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
title_fullStr |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
title_full_unstemmed |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
title_sort |
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows |
dc.creator.none.fl_str_mv |
Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Verónica Bandoni, Arnaldo Luis Marchetti, María Laura Mestorino, Olga Nora |
author |
Buldain, Daniel Cornelio |
author_facet |
Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Verónica Bandoni, Arnaldo Luis Marchetti, María Laura Mestorino, Olga Nora |
author_role |
author |
author2 |
Gortari Castillo, Lihuel Buchamer, Andrea Verónica Bandoni, Arnaldo Luis Marchetti, María Laura Mestorino, Olga Nora |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Melaleuca armillaris essential oil erythromycin synergism Staphylococcus aureus mastitis |
topic |
Ciencias Veterinarias Melaleuca armillaris essential oil erythromycin synergism Staphylococcus aureus mastitis |
dc.description.none.fl_txt_mv |
Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus. Facultad de Ciencias Veterinarias |
description |
Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-15 |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/153603 |
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eng |
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eng |
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