Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data
- Autores
- Debat, Humberto; García, María Laura; Bejerman, Nicolás
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
Plant viruses
Ophiovirus
Virus taxonomy
Metatranscriptomics
Virus discovery - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/153023
Ver los metadatos del registro completo
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Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics dataDebat, HumbertoGarcía, María LauraBejerman, NicolásBiologíaPlant virusesOphiovirusVirus taxonomyMetatranscriptomicsVirus discoveryOphioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.Instituto de Biotecnología y Biología Molecular2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/153023enginfo:eu-repo/semantics/altIdentifier/issn/1999-4915info:eu-repo/semantics/altIdentifier/doi/10.3390/v15040840info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:39:34Zoai:sedici.unlp.edu.ar:10915/153023Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:39:34.405SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
title |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
spellingShingle |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data Debat, Humberto Biología Plant viruses Ophiovirus Virus taxonomy Metatranscriptomics Virus discovery |
title_short |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
title_full |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
title_fullStr |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
title_full_unstemmed |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
title_sort |
Expanding the repertoire of the plant-infecting ophioviruses through metatranscriptomics data |
dc.creator.none.fl_str_mv |
Debat, Humberto García, María Laura Bejerman, Nicolás |
author |
Debat, Humberto |
author_facet |
Debat, Humberto García, María Laura Bejerman, Nicolás |
author_role |
author |
author2 |
García, María Laura Bejerman, Nicolás |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Biología Plant viruses Ophiovirus Virus taxonomy Metatranscriptomics Virus discovery |
topic |
Biología Plant viruses Ophiovirus Virus taxonomy Metatranscriptomics Virus discovery |
dc.description.none.fl_txt_mv |
Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. Instituto de Biotecnología y Biología Molecular |
description |
Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with nonenveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
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http://sedici.unlp.edu.ar/handle/10915/153023 |
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eng |
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