Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome

Autores
Debat, Humberto Julio; Paolinelli, Marcos; Escoriaza, Maria Georgina; Garcia Lampasona, Sandra Claudia; Gomez Talquenca, Gonzalo; Bejerman, Nicolas Esteban
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Given the agronomic and economic importance of viticulture, grapevine has been shown to host the largest number of viruses among plants to date. Nevertheless, studies assessing the grapevine-associated holobiont remain scarce. In this context, the viral component of this ecological niche is understudied. In this work, through metatranscriptomics of wood samples from individual grapevines that were either healthy or exhibited symptoms of grapevine trunk disease from Argentina, we provide a glimpse into the wood linked virome. Virus discovery from high-throughput sequencing data resulted in the identification and reconstruction of 123 novel virus sequences. Genetic and phylogenetic insights suggest that these sequences correspond to 78 novel virus species. Structural and functional annotation of the viruses showed a great diversity of genomic organizations, with the presence of dsRNA, ssRNA( ) and ssRNA(+) viruses belonging to more than 15 virus families. Some highly divergent viruses resembling narnaviruses, ophioviruses, deltaflexiviruses and bunyaviruses could be accommodated within new genera or even new virus families. The differential detection and variable RNA levels across samples suggest complex dynamics and prevalence patterns of those novel viruses. The viral profile described here provides a first insight into the multifaceted South American grapevine wood holobiont mycovirome.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza (IBAM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnica. Instituto de Biología Agrícola de Mendoza (IBAM); Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Virology 610 : 110604 (September 2025)
Materia
Vid
Grapevines
Transcriptomics
Transcriptómica
Mycovirome
Holobiont
Metatranscriptomics
Virus Diversity
Viral Taxonomy
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22834
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oai_identifier_str oai:localhost:20.500.12123/22834
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network_name_str INTA Digital (INTA)
spelling Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycoviromeDebat, Humberto JulioPaolinelli, MarcosEscoriaza, Maria GeorginaGarcia Lampasona, Sandra ClaudiaGomez Talquenca, GonzaloBejerman, Nicolas EstebanVidGrapevinesTranscriptomicsTranscriptómicaMycoviromeHolobiontMetatranscriptomicsVirus DiversityViral TaxonomyGiven the agronomic and economic importance of viticulture, grapevine has been shown to host the largest number of viruses among plants to date. Nevertheless, studies assessing the grapevine-associated holobiont remain scarce. In this context, the viral component of this ecological niche is understudied. In this work, through metatranscriptomics of wood samples from individual grapevines that were either healthy or exhibited symptoms of grapevine trunk disease from Argentina, we provide a glimpse into the wood linked virome. Virus discovery from high-throughput sequencing data resulted in the identification and reconstruction of 123 novel virus sequences. Genetic and phylogenetic insights suggest that these sequences correspond to 78 novel virus species. Structural and functional annotation of the viruses showed a great diversity of genomic organizations, with the presence of dsRNA, ssRNA( ) and ssRNA(+) viruses belonging to more than 15 virus families. Some highly divergent viruses resembling narnaviruses, ophioviruses, deltaflexiviruses and bunyaviruses could be accommodated within new genera or even new virus families. The differential detection and variable RNA levels across samples suggest complex dynamics and prevalence patterns of those novel viruses. The viral profile described here provides a first insight into the multifaceted South American grapevine wood holobiont mycovirome.Instituto de Patología VegetalFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza (IBAM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnica. Instituto de Biología Agrícola de Mendoza (IBAM); ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaElsevier2025-06-30T16:50:05Z2025-06-30T16:50:05Z2025-06-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22834https://www.sciencedirect.com/science/article/pii/S004268222500217X0042-6822https://doi.org/10.1016/j.virol.2025.110604Virology 610 : 110604 (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambientalinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:22Zoai:localhost:20.500.12123/22834instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:23.575INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
title Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
spellingShingle Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
Debat, Humberto Julio
Vid
Grapevines
Transcriptomics
Transcriptómica
Mycovirome
Holobiont
Metatranscriptomics
Virus Diversity
Viral Taxonomy
title_short Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
title_full Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
title_fullStr Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
title_full_unstemmed Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
title_sort Grapevine holobiome metatranscriptomics provides a glimpse into the wood mycovirome
dc.creator.none.fl_str_mv Debat, Humberto Julio
Paolinelli, Marcos
Escoriaza, Maria Georgina
Garcia Lampasona, Sandra Claudia
Gomez Talquenca, Gonzalo
Bejerman, Nicolas Esteban
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Paolinelli, Marcos
Escoriaza, Maria Georgina
Garcia Lampasona, Sandra Claudia
Gomez Talquenca, Gonzalo
Bejerman, Nicolas Esteban
author_role author
author2 Paolinelli, Marcos
Escoriaza, Maria Georgina
Garcia Lampasona, Sandra Claudia
Gomez Talquenca, Gonzalo
Bejerman, Nicolas Esteban
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Vid
Grapevines
Transcriptomics
Transcriptómica
Mycovirome
Holobiont
Metatranscriptomics
Virus Diversity
Viral Taxonomy
topic Vid
Grapevines
Transcriptomics
Transcriptómica
Mycovirome
Holobiont
Metatranscriptomics
Virus Diversity
Viral Taxonomy
dc.description.none.fl_txt_mv Given the agronomic and economic importance of viticulture, grapevine has been shown to host the largest number of viruses among plants to date. Nevertheless, studies assessing the grapevine-associated holobiont remain scarce. In this context, the viral component of this ecological niche is understudied. In this work, through metatranscriptomics of wood samples from individual grapevines that were either healthy or exhibited symptoms of grapevine trunk disease from Argentina, we provide a glimpse into the wood linked virome. Virus discovery from high-throughput sequencing data resulted in the identification and reconstruction of 123 novel virus sequences. Genetic and phylogenetic insights suggest that these sequences correspond to 78 novel virus species. Structural and functional annotation of the viruses showed a great diversity of genomic organizations, with the presence of dsRNA, ssRNA( ) and ssRNA(+) viruses belonging to more than 15 virus families. Some highly divergent viruses resembling narnaviruses, ophioviruses, deltaflexiviruses and bunyaviruses could be accommodated within new genera or even new virus families. The differential detection and variable RNA levels across samples suggest complex dynamics and prevalence patterns of those novel viruses. The viral profile described here provides a first insight into the multifaceted South American grapevine wood holobiont mycovirome.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza (IBAM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnica. Instituto de Biología Agrícola de Mendoza (IBAM); Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Given the agronomic and economic importance of viticulture, grapevine has been shown to host the largest number of viruses among plants to date. Nevertheless, studies assessing the grapevine-associated holobiont remain scarce. In this context, the viral component of this ecological niche is understudied. In this work, through metatranscriptomics of wood samples from individual grapevines that were either healthy or exhibited symptoms of grapevine trunk disease from Argentina, we provide a glimpse into the wood linked virome. Virus discovery from high-throughput sequencing data resulted in the identification and reconstruction of 123 novel virus sequences. Genetic and phylogenetic insights suggest that these sequences correspond to 78 novel virus species. Structural and functional annotation of the viruses showed a great diversity of genomic organizations, with the presence of dsRNA, ssRNA( ) and ssRNA(+) viruses belonging to more than 15 virus families. Some highly divergent viruses resembling narnaviruses, ophioviruses, deltaflexiviruses and bunyaviruses could be accommodated within new genera or even new virus families. The differential detection and variable RNA levels across samples suggest complex dynamics and prevalence patterns of those novel viruses. The viral profile described here provides a first insight into the multifaceted South American grapevine wood holobiont mycovirome.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-30T16:50:05Z
2025-06-30T16:50:05Z
2025-06-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22834
https://www.sciencedirect.com/science/article/pii/S004268222500217X
0042-6822
https://doi.org/10.1016/j.virol.2025.110604
url http://hdl.handle.net/20.500.12123/22834
https://www.sciencedirect.com/science/article/pii/S004268222500217X
https://doi.org/10.1016/j.virol.2025.110604
identifier_str_mv 0042-6822
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambiental
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Virology 610 : 110604 (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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