Comprehensive evaluation and implementation of improvement actions in butcher shops

Autores
Leotta, Gerardo Aníbal; Brusa, Victoria; Galli, Lucía; Adriani, Cristian; Linares, Luciano Héctor; Etcheverría, Analía; Sanz, Marcelo E.; Sucari, Adriana; Peral García, Pilar; Signorini, Marcelo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or low-risk (71-100). A total of 172 raw ground beef and 672 environmental samples were collected from 86 butcher shops during the evaluation (2010-2011) and verification (2013) stages of the study. Ground beef samples were analyzed for mesophilic aerobic organisms, Escherichia coli and coagulase-positive Staphylococcus aureus enumeration. Salmonella spp., E. coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), and Listeria monocytogenes were detected and isolated from all samples. Risk quantification resulted in 43 (50.0%) high-risk, 34 (39.5%) moderate-risk, and nine (10.5%) low-risk butcher shops. Training sessions for 498 handlers and 4,506 consumers were held. Re-evaluation by risk quantification and microbiological analyses resulted in 19 (22.1%) high-risk, 42 (48.8%) moderate-risk and 25 (29.1%) low-risk butcher shops. The count of indicator microorganisms decreased with respect to the 2010-2011 period. After the implementation of improvement actions, the presence of L. monocytogenes, E. coli O157:H7 and stx genes in ground beef decreased. Salmonella spp. was isolated from 10 (11.6%) ground beef samples, without detecting statistically significant differences between both study periods (evaluation and verification). The percentage of pathogens in environmental samples was reduced in the verification period (Salmonella spp., 1.5%; L. monocytogenes, 10.7%; E. coli O157:H7, 0.6%; non-O157 STEC, 6.8%). Risk quantification was useful to identify those relevant facts in butcher shops. The reduction of contamination in ground beef and the environment was possible after training handlers based on the problems identified in their own butcher shops. Our results confirm the feasibility of implementing a comprehensive risk management program in butcher shops, and the importance of information campaigns targeting consumers. Further collaborative efforts would be necessary to improve foodstuffs safety at retail level and at home.
Facultad de Ciencias Veterinarias
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
Butcher Shops
Foodborne pathogens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86513

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network_name_str SEDICI (UNLP)
spelling Comprehensive evaluation and implementation of improvement actions in butcher shopsLeotta, Gerardo AníbalBrusa, VictoriaGalli, LucíaAdriani, CristianLinares, Luciano HéctorEtcheverría, AnalíaSanz, Marcelo E.Sucari, AdrianaPeral García, PilarSignorini, MarceloCiencias VeterinariasButcher ShopsFoodborne pathogensFoodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or low-risk (71-100). A total of 172 raw ground beef and 672 environmental samples were collected from 86 butcher shops during the evaluation (2010-2011) and verification (2013) stages of the study. Ground beef samples were analyzed for mesophilic aerobic organisms, <i>Escherichia coli</i> and coagulase-positive <i>Staphylococcus aureus</i> enumeration. <i>Salmonella</i> spp., <i>E. coli</i> O157:H7, non-O157 Shiga toxin-producing <i>E. coli</i> (STEC), and <i>Listeria monocytogenes</i> were detected and isolated from all samples. Risk quantification resulted in 43 (50.0%) high-risk, 34 (39.5%) moderate-risk, and nine (10.5%) low-risk butcher shops. Training sessions for 498 handlers and 4,506 consumers were held. Re-evaluation by risk quantification and microbiological analyses resulted in 19 (22.1%) high-risk, 42 (48.8%) moderate-risk and 25 (29.1%) low-risk butcher shops. The count of indicator microorganisms decreased with respect to the 2010-2011 period. After the implementation of improvement actions, the presence of <i>L. monocytogenes</i>, <i>E. coli</i> O157:H7 and stx genes in ground beef decreased. <i>Salmonella</i> spp. was isolated from 10 (11.6%) ground beef samples, without detecting statistically significant differences between both study periods (evaluation and verification). The percentage of pathogens in environmental samples was reduced in the verification period (<i>Salmonella</i> spp., 1.5%; <i>L. monocytogenes</i>, 10.7%; <i>E. coli</i> O157:H7, 0.6%; non-O157 STEC, 6.8%). Risk quantification was useful to identify those relevant facts in butcher shops. The reduction of contamination in ground beef and the environment was possible after training handlers based on the problems identified in their own butcher shops. Our results confirm the feasibility of implementing a comprehensive risk management program in butcher shops, and the importance of information campaigns targeting consumers. Further collaborative efforts would be necessary to improve foodstuffs safety at retail level and at home.Facultad de Ciencias VeterinariasInstituto de Genética Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86513enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0162635info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:44Zoai:sedici.unlp.edu.ar:10915/86513Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:44.685SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Comprehensive evaluation and implementation of improvement actions in butcher shops
title Comprehensive evaluation and implementation of improvement actions in butcher shops
spellingShingle Comprehensive evaluation and implementation of improvement actions in butcher shops
Leotta, Gerardo Aníbal
Ciencias Veterinarias
Butcher Shops
Foodborne pathogens
title_short Comprehensive evaluation and implementation of improvement actions in butcher shops
title_full Comprehensive evaluation and implementation of improvement actions in butcher shops
title_fullStr Comprehensive evaluation and implementation of improvement actions in butcher shops
title_full_unstemmed Comprehensive evaluation and implementation of improvement actions in butcher shops
title_sort Comprehensive evaluation and implementation of improvement actions in butcher shops
dc.creator.none.fl_str_mv Leotta, Gerardo Aníbal
Brusa, Victoria
Galli, Lucía
Adriani, Cristian
Linares, Luciano Héctor
Etcheverría, Analía
Sanz, Marcelo E.
Sucari, Adriana
Peral García, Pilar
Signorini, Marcelo
author Leotta, Gerardo Aníbal
author_facet Leotta, Gerardo Aníbal
Brusa, Victoria
Galli, Lucía
Adriani, Cristian
Linares, Luciano Héctor
Etcheverría, Analía
Sanz, Marcelo E.
Sucari, Adriana
Peral García, Pilar
Signorini, Marcelo
author_role author
author2 Brusa, Victoria
Galli, Lucía
Adriani, Cristian
Linares, Luciano Héctor
Etcheverría, Analía
Sanz, Marcelo E.
Sucari, Adriana
Peral García, Pilar
Signorini, Marcelo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Butcher Shops
Foodborne pathogens
topic Ciencias Veterinarias
Butcher Shops
Foodborne pathogens
dc.description.none.fl_txt_mv Foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or low-risk (71-100). A total of 172 raw ground beef and 672 environmental samples were collected from 86 butcher shops during the evaluation (2010-2011) and verification (2013) stages of the study. Ground beef samples were analyzed for mesophilic aerobic organisms, <i>Escherichia coli</i> and coagulase-positive <i>Staphylococcus aureus</i> enumeration. <i>Salmonella</i> spp., <i>E. coli</i> O157:H7, non-O157 Shiga toxin-producing <i>E. coli</i> (STEC), and <i>Listeria monocytogenes</i> were detected and isolated from all samples. Risk quantification resulted in 43 (50.0%) high-risk, 34 (39.5%) moderate-risk, and nine (10.5%) low-risk butcher shops. Training sessions for 498 handlers and 4,506 consumers were held. Re-evaluation by risk quantification and microbiological analyses resulted in 19 (22.1%) high-risk, 42 (48.8%) moderate-risk and 25 (29.1%) low-risk butcher shops. The count of indicator microorganisms decreased with respect to the 2010-2011 period. After the implementation of improvement actions, the presence of <i>L. monocytogenes</i>, <i>E. coli</i> O157:H7 and stx genes in ground beef decreased. <i>Salmonella</i> spp. was isolated from 10 (11.6%) ground beef samples, without detecting statistically significant differences between both study periods (evaluation and verification). The percentage of pathogens in environmental samples was reduced in the verification period (<i>Salmonella</i> spp., 1.5%; <i>L. monocytogenes</i>, 10.7%; <i>E. coli</i> O157:H7, 0.6%; non-O157 STEC, 6.8%). Risk quantification was useful to identify those relevant facts in butcher shops. The reduction of contamination in ground beef and the environment was possible after training handlers based on the problems identified in their own butcher shops. Our results confirm the feasibility of implementing a comprehensive risk management program in butcher shops, and the importance of information campaigns targeting consumers. Further collaborative efforts would be necessary to improve foodstuffs safety at retail level and at home.
Facultad de Ciencias Veterinarias
Instituto de Genética Veterinaria
description Foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or low-risk (71-100). A total of 172 raw ground beef and 672 environmental samples were collected from 86 butcher shops during the evaluation (2010-2011) and verification (2013) stages of the study. Ground beef samples were analyzed for mesophilic aerobic organisms, <i>Escherichia coli</i> and coagulase-positive <i>Staphylococcus aureus</i> enumeration. <i>Salmonella</i> spp., <i>E. coli</i> O157:H7, non-O157 Shiga toxin-producing <i>E. coli</i> (STEC), and <i>Listeria monocytogenes</i> were detected and isolated from all samples. Risk quantification resulted in 43 (50.0%) high-risk, 34 (39.5%) moderate-risk, and nine (10.5%) low-risk butcher shops. Training sessions for 498 handlers and 4,506 consumers were held. Re-evaluation by risk quantification and microbiological analyses resulted in 19 (22.1%) high-risk, 42 (48.8%) moderate-risk and 25 (29.1%) low-risk butcher shops. The count of indicator microorganisms decreased with respect to the 2010-2011 period. After the implementation of improvement actions, the presence of <i>L. monocytogenes</i>, <i>E. coli</i> O157:H7 and stx genes in ground beef decreased. <i>Salmonella</i> spp. was isolated from 10 (11.6%) ground beef samples, without detecting statistically significant differences between both study periods (evaluation and verification). The percentage of pathogens in environmental samples was reduced in the verification period (<i>Salmonella</i> spp., 1.5%; <i>L. monocytogenes</i>, 10.7%; <i>E. coli</i> O157:H7, 0.6%; non-O157 STEC, 6.8%). Risk quantification was useful to identify those relevant facts in butcher shops. The reduction of contamination in ground beef and the environment was possible after training handlers based on the problems identified in their own butcher shops. Our results confirm the feasibility of implementing a comprehensive risk management program in butcher shops, and the importance of information campaigns targeting consumers. Further collaborative efforts would be necessary to improve foodstuffs safety at retail level and at home.
publishDate 2016
dc.date.none.fl_str_mv 2016
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0162635
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