Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

Autores
Medina, Rocío; Lucentini, César Gustavo; Franco, Mario Emilio Ernesto; Petroselli, Gabriela; Rosso, Janina Alejandra; Erra Balsells, Rosa; Balatti, Pedro Alberto; Saparrat, Mario Carlos Nazareno
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
Centro de Investigaciones de Fitopatología
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Instituto de Fisiología Vegetal
Instituto de Botánica "Dr. Carlos Spegazzini"
Materia
Biología
Biochemistry
Genetics
Microbiology
Molecular biology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/97948
Acceder
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network_name_str SEDICI (UNLP)
spelling Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)Medina, RocíoLucentini, César GustavoFranco, Mario Emilio ErnestoPetroselli, GabrielaRosso, Janina AlejandraErra Balsells, RosaBalatti, Pedro AlbertoSaparrat, Mario Carlos NazarenoBiologíaBiochemistryGeneticsMicrobiologyMolecular biologyFulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.Centro de Investigaciones de FitopatologíaInstituto de Investigaciones Fisicoquímicas Teóricas y AplicadasInstituto de Fisiología VegetalInstituto de Botánica "Dr. Carlos Spegazzini"2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-21http://sedici.unlp.edu.ar/handle/10915/97948enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/88783info:eu-repo/semantics/altIdentifier/issn/2405-8440info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2018.e01036info:eu-repo/semantics/altIdentifier/hdl/11336/88783info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:52:46Zoai:sedici.unlp.edu.ar:10915/97948Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:52:47.246SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
spellingShingle Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
Medina, Rocío
Biología
Biochemistry
Genetics
Microbiology
Molecular biology
title_short Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_fullStr Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_full_unstemmed Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
title_sort Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)
dc.creator.none.fl_str_mv Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author Medina, Rocío
author_facet Medina, Rocío
Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author_role author
author2 Lucentini, César Gustavo
Franco, Mario Emilio Ernesto
Petroselli, Gabriela
Rosso, Janina Alejandra
Erra Balsells, Rosa
Balatti, Pedro Alberto
Saparrat, Mario Carlos Nazareno
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Biochemistry
Genetics
Microbiology
Molecular biology
topic Biología
Biochemistry
Genetics
Microbiology
Molecular biology
dc.description.none.fl_txt_mv Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
Centro de Investigaciones de Fitopatología
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Instituto de Fisiología Vegetal
Instituto de Botánica "Dr. Carlos Spegazzini"
description Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/97948
url http://sedici.unlp.edu.ar/handle/10915/97948
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/88783
info:eu-repo/semantics/altIdentifier/issn/2405-8440
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2018.e01036
info:eu-repo/semantics/altIdentifier/hdl/11336/88783
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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